FUNCTION: Activates ubiquitin by first adenylating with ATP its carboxy-terminal glycine residue and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin-E1 thiolester and free AMP. * PATHWAY: Ubiquitin conjugation; first step. * SUBUNIT: Monomer (By similarity). * MISCELLANEOUS: THERE ARE TWO ACTIVE SITES WITHIN THE E1 MOLECULE, ALLOWING IT TO ACCOMODATE TWO UBIQUITIN MOIETIES AT A TIME, WITH A NEW UBIQUITIN FORMING AN ADENYLATE INTERMEDIATE AS THE PREVIOUS ONE IS TRANSFERRED TO THE THIOL SITE. * SIMILARITY: BELONGS TO THE UBIQUITIN-ACTIVATING E1 FAMILY. |
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10-DEC-02
Last Update 13-Mar-03