MS-Fit Search Results



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Parameters

Database searched: SwissProt.10.25.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 2
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): HSG-081
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 116819
Molecular weight search (1000 - 100000 Da) selects 110413 entries.
Full pI range: 116819 entries.
Species search ( HUMAN MOUSE ) selects 14257 entries.
Combined molecular weight, pI and species searches select 12612 entries.
Pre searches select 12612 entries.

Data Set 1 Results

MS-Fit search selects 28 entries (results displayed for top 5 matches).

Results Summary

MOWSE
Score
#/35(%)
Masses
Matched
%
Cov
%
TIC
Mean
Err
ppm
Data
Tol
ppm
MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
156.04 (11)15.011.4-63.816.2 34196/9.3MOUSEHeterogeneous nuclear ribonucleoprotein A1 (Helix-destabilizing protein) (Single-strand binding protein) (hnRNP core protein A1) (HDP-1) (Topoisomerase-inhibitor suppressed)
249.34 (11)13.011.4-63.816.2 38846/9.3HUMANHeterogeneous nuclear ribonucleoprotein A1 (Helix-destabilizing protein) (Single-strand binding protein) (hnRNP core protein A1)
330.05 (14)10.014.3-70.659.0 89742/5.5HUMANPERQ amino acid rich with GYF domain protein 1
426.04 (11)5.011.4-64.031.4 99104/5.5HUMANCone cGMP-specific 3',5'-cyclic phosphodiesterase alpha'-subunit
526.04 (11)9.011.4-43.740.9 60058/5.8MOUSEProto-oncogene tyrosine-protein kinase FYN (P59-FYN)
107020......................x.x....x..x..
105172......................x.x....x..x..
29659....x.........x.......x.x.........x
13125....x.........x.......x...x........
62338...........................xx.x..x.



Detailed Results

1. 4/35 matches (11%).
Acc. #: Species: MOUSE Name: Heterogeneous nuclear ribonucleoprotein A1 (Helix-destabilizing protein) (Single-strand binding protein) (hnRNP core protein A1) (HDP-1) (Topoisomerase-inhibitor suppressed)
Index: MW: 34196 Da pI: 9.3
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1556.67411556.7920-76AcetN 1142(-)SKSESPKEPEQLR(K)
1628.68521628.7821-602853000(R) (N)
1694.59611694.7006-623013180(R) (R)
1997.75391997.8702-583013202(R) (-)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 15% (50/320AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 4/35 matches (11%).
Acc. #: Species: HUMAN Name: Heterogeneous nuclear ribonucleoprotein A1 (Helix-destabilizing protein) (Single-strand binding protein) (hnRNP core protein A1)
Index: MW: 38846 Da pI: 9.3
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1556.67411556.7920-76AcetN 1142(-)SKSESPKEPEQLR(K)
1628.68521628.7821-603373520(R) (N)
1694.59611694.7006-623533700(R) (R)
1997.75391997.8702-583533722(R) (-)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 13% (50/372AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 5/35 matches (14%).
Acc. #: Species: HUMAN Name: PERQ amino acid rich with GYF domain protein 1
Index: MW: 89742 Da pI: 5.5
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
964.3551964.3794-251691770(R) (G)
1021.36461021.4478-811901980(R) (D)
1556.67411556.8185-9313242(R) (E)
1628.68521628.7530-421321462(R) (S)
1628.68521628.8318-9060752(K) (S)
2283.00432283.2151-923383582(R) (D)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 10% (82/817AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 4/35 matches (11%).
Acc. #: Species: HUMAN Name: Cone cGMP-specific 3',5'-cyclic phosphodiesterase alpha'-subunit
Index: MW: 99104 Da pI: 5.5
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
964.3551964.4264-74pyroGlu 2082150(K)QDEEVFSK(Y)
1021.36461021.4074-428408500(K) (K)
1556.67411556.7929-767547660(R) (N)
1650.66411650.7698-642262380(R) (R)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 5% (45/858AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 4/35 matches (11%).
Acc. #: Species: MOUSE Name: Proto-oncogene tyrosine-protein kinase FYN (P59-FYN)
Index: MW: 60058 Da pI: 5.8
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1656.67551656.7692-571831961(K) (G)
1672.65991672.7641-621Met-ox 1831961(K)GAYSLSIRDWDDMK(G)
1837.82001837.8509-171091230(K) (S)
2210.97822211.0642-3910292(K) (Y)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 9% (49/534AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):


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