MS-Fit Search Results



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Parameters

Database searched: SwissProt.10.25.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 2
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): HSG-083
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 116819
Molecular weight search (1000 - 100000 Da) selects 110413 entries.
Full pI range: 116819 entries.
Species search ( HUMAN MOUSE ) selects 14257 entries.
Combined molecular weight, pI and species searches select 12612 entries.
Pre searches select 12612 entries.

Data Set 1 Results

MS-Fit search selects 118 entries (results displayed for top 5 matches).

Results Summary

MOWSE
Score
#/27(%)
Masses
Matched
%
Cov
%
TIC
Mean
Err
ppm
Data
Tol
ppm
MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
123638 (29)24.029.622.813.0 34196/9.3MOUSEHeterogeneous nuclear ribonucleoprotein A1 (Helix-destabilizing protein) (Single-strand binding protein) (hnRNP core protein A1) (HDP-1) (Topoisomerase-inhibitor suppressed)
220818 (29)20.029.622.813.0 38846/9.3HUMANHeterogeneous nuclear ribonucleoprotein A1 (Helix-destabilizing protein) (Single-strand binding protein) (hnRNP core protein A1)
31964 (14)10.014.8-5.6467.7 53575/9.0MOUSECytochrome P450 39A1 (Oxysterol 7-alpha-hydroxylase) (mCYP39A1)
41945 (18)12.018.5-3.1149.5 63770/8.7MOUSESqualene monooxygenase (Squalene epoxidase) (SE)
51314 (14)16.014.87.3377.0 36971/5.4MOUSEDNA repair protein RAD51 homolog 1
107020..x.xx.x...x......xx.x.....
105172..x.xx.x...x......xx.x.....
63522.......x...x....x......x...
28122.....xx..........xx.x......
53329................xx.x...x...



Detailed Results

1. 8/27 matches (29%).
Acc. #: Species: MOUSE Name: Heterogeneous nuclear ribonucleoprotein A1 (Helix-destabilizing protein) (Single-strand binding protein) (hnRNP core protein A1) (HDP-1) (Topoisomerase-inhibitor suppressed)
Index: MW: 34196 Da pI: 9.3
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1299.67811299.6545184141(K) (K)
1427.78971427.7494284152(K) (L)
1437.76211437.745012931050(R) (K)
1556.82311556.792020AcetN 1142(-)SKSESPKEPEQLR(K)
1628.82561628.7821272853000(R) (N)
1694.75291694.7006313013180(R) (R)
1784.94111784.90711916310(K) (S)
1913.05451913.00202715311(R) (S)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 24% (78/320AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 8/27 matches (29%).
Acc. #: Species: HUMAN Name: Heterogeneous nuclear ribonucleoprotein A1 (Helix-destabilizing protein) (Single-strand binding protein) (hnRNP core protein A1)
Index: MW: 38846 Da pI: 9.3
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1299.67811299.6545184141(K) (K)
1427.78971427.7494284152(K) (L)
1437.76211437.745012931050(R) (K)
1556.82311556.792020AcetN 1142(-)SKSESPKEPEQLR(K)
1628.82561628.7821273373520(R) (N)
1694.75291694.7006313533700(R) (R)
1784.94111784.90711916310(K) (S)
1913.05451913.00202715311(R) (S)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 20% (78/372AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 4/27 matches (14%).
Acc. #: Species: MOUSE Name: Cytochrome P450 39A1 (Oxysterol 7-alpha-hydroxylase) (mCYP39A1)
Index: MW: 53575 Da pI: 9.0
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1556.82311556.8728-321671790(R) (K)
1628.82561628.7823273994120(K) (F)
1684.90431684.9678-381671801(R) (G)
2527.27752527.225820911120(K) (N)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 10% (50/470AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 5/27 matches (18%).
Acc. #: Species: MOUSE Name: Squalene monooxygenase (Squalene epoxidase) (SE)
Index: MW: 63770 Da pI: 8.7
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1437.76211437.75643.92983100(K) (D)
1475.78951475.7568223853980(R) (R)
1685.85961685.8647-3.05555680(K) (Y)
1694.75291694.8277-444354490(K) (K)
1837.97711837.96685.62Met-ox 2272422(R)FIMSLRKAAMAEPNVK(F)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 12% (72/572AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 4/27 matches (14%).
Acc. #: Species: MOUSE Name: DNA repair protein RAD51 homolog 1
Index: MW: 36971 Da pI: 5.4
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1684.90431684.9620-342422542(R) (L)
1685.85961685.7957381571700(K) (L)
1784.94111784.9706-172362502(R) (M)
2527.27752527.1710421942150(R) (Y)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 16% (55/339AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):


MS-Fit in ProteinProspector 4.0.4
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