MS-Fit Search Results

MS-Fit Search Results

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Parameters

Database searched: SwissProt.10.25.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): 086
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 116819
Molecular weight search (1000 - 100000 Da) selects 110413 entries.
Full pI range: 116819 entries.
Species search ( HUMAN MOUSE ) selects 14257 entries.
Combined molecular weight, pI and species searches select 12612 entries.
Pre searches select 12612 entries.

Data Set 1 Results

MS-Fit search selects 187 entries (results displayed for top 5 matches).

Results Summary

	MOWSE Score	#/64(%) Masses Matched	% Cov	% TIC	Mean Err ppm	Data Tol ppm	MS-Digest Index #	Protein MW (Da)/pI	Accession #	Species	Protein Name
1	5.117e+008	14 (21)	42.0	21.9	-2.91	28.1		35993/8.7		MOUSE	Heterogeneous nuclear ribonucleoproteins A2/B1 (hnRNP A2 / hnRNP B1)
2	4.921e+008	14 (21)	41.0	21.9	-2.91	28.1		37430/9.0		HUMAN	Heterogeneous nuclear ribonucleoproteins A2/B1 (hnRNP A2 / hnRNP B1)
3	1.421e+004	8 (12)	18.0	12.5	-4.75	36.2		66018/8.2		HUMAN	Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
4	1797	5 (7)	5.0	7.8	-6.45	46.4		78000/5.8		MOUSE	ARACHIDONATE 5-LIPOXYGENASE (5-LIPOXYGENASE) (5-LO)
5	873	5 (7)	15.0	7.8	-8.22	41.1		40478/7.9		MOUSE	Proteoglycan link protein precursor (Cartilage link protein) (LP)

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Detailed Results

1. 14/64 matches (21%).
Acc. #: Species: MOUSE Name: Heterogeneous nuclear ribonucleoproteins A2/B1 (hnRNP A2 / hnRNP B1)
Index: MW: 35993 Da pI: 8.7

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
1013.4420	1013.4441	-2.0		192	201	0	(R) (G)
1087.4800	1087.4849	-4.5		27	34	0	(R) (L)
1237.5546	1237.5483	5.1	1Met-ox	179	188	0	(R)QEMQEVQSSR(S)
1313.5734	1313.5987	-19		189	201	1	(R) (G)
1377.6281	1377.6300	-1.4		202	216	0	(R) (G)
1410.6918	1410.6878	2.8		162	173	0	(K) (K)
1538.7432	1538.7828	-26		162	174	1	(K) (A)
1695.7664	1695.7655	0.54		142	156	0	(R) (I)
1798.9265	1798.9227	2.1		11	26	0	(K) (N)
1851.9243	1851.8666	31		141	156	1	(K) (I)
1927.0078	1927.0177	-5.1		10	26	1	(R) (N)
2189.8754	2189.9059	-14		314	338	0	(R) (S)
2205.9181	2205.9008	7.8	1Met-ox	314	338	0	(R)NMGGPYGGGNYGPGGSGGSGGYGGR(S)
2494.9945	2495.0401	-18		227	254	0	(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 42% (145/341AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 14/64 matches (21%).
Acc. #: Species: HUMAN Name: Heterogeneous nuclear ribonucleoproteins A2/B1 (hnRNP A2 / hnRNP B1)
Index: MW: 37430 Da pI: 9.0

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1013.4420 1013.4441 -2.0 204 213 0 (R) (G)
1087.4800 1087.4849 -4.5 39 46 0 (R) (L)
1237.5546 1237.5483 5.1 1Met-ox 191 200 0 (R)QEMQEVQSSR(S)
1313.5734 1313.5987 -19 201 213 1 (R) (G)
1377.6281 1377.6300 -1.4 214 228 0 (R) (G)
1410.6918 1410.6878 2.8 174 185 0 (K) (K)
1538.7432 1538.7828 -26 174 186 1 (K) (A)
1695.7664 1695.7655 0.54 154 168 0 (R) (I)
1798.9265 1798.9227 2.1 23 38 0 (K) (N)
1851.9243 1851.8666 31 153 168 1 (K) (I)
1927.0078 1927.0177 -5.1 22 38 1 (R) (N)
2189.8754 2189.9059 -14 326 350 0 (R) (S)
2205.9181 2205.9008 7.8 1Met-ox 326 350 0 (R)NMGGPYGGGNYGPGGSGGSGGYGGR(S)
2494.9945 2495.0401 -18 239 266 0 (R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 41% (145/353AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 8/64 matches (12%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
Index: MW: 66018 Da pI: 8.2

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1087.4800 1087.5285 -45 4 12 1 (R) (S)
1179.5914 1179.6010 -8.1 377 386 0 (K) (H)
1300.5437 1300.5302 10 258 267 0 (K) (N)
1357.7105 1357.6963 10 444 455 0 (K) (E)
1393.7192 1393.7327 -9.7 278 289 1 (R) (D)
1475.7597 1475.7494 7.0 212 223 0 (K) (T)
1475.7597 1475.7858 -18 200 211 0 (R) (W)
1638.8598 1638.8604 -0.33 186 199 1 (K) (F)
2383.9760 2383.9524 9.9 519 549 0 (R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 18% (122/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 5/64 matches (7%).
Acc. #: Species: MOUSE Name: ARACHIDONATE 5-LIPOXYGENASE (5-LIPOXYGENASE) (5-LO)
Index: MW: 78000 Da pI: 5.8

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1107.5189 1107.5621 -39 185 193 0 (K) (F)
1638.8598 1638.8314 17 1Met-ox 372 385 0 (R)THLVSEVFGIAMYR(Q)
2189.8754 2189.9213 -21 194 210 0 (R) (I)
2205.9181 2205.9163 0.82 1Met-ox 194 210 0 (R)FMHMFQSSWHDFADFEK(I)
2221.9325 2221.9112 9.6 2Met-ox 194 210 0 (R)FMHMFQSSWHDFADFEK(I)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 5% (40/674AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 5/64 matches (7%).
Acc. #: Species: MOUSE Name: Proteoglycan link protein precursor (Cartilage link protein) (LP)
Index: MW: 40478 Da pI: 7.9

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1172.4968 1172.5376 -35 239 247 1 (R) (S)
1320.5804 1320.6007 -15 306 317 0 (R) (Y)
1410.6918 1410.6728 13 93 104 0 (R) (K)
1538.7432 1538.7677 -16 93 105 1 (R) (T)
2211.0675 2211.0417 12 118 138 0 (K) (Y)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 15% (55/356AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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