MS-Fit Search Results

MS-Fit Search Results

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Parameters

Database searched: SwissProt.10.25.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): HSG-099
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 116819
Molecular weight search (1000 - 100000 Da) selects 110413 entries.
Full pI range: 116819 entries.
Species search ( HUMAN MOUSE ) selects 14257 entries.
Combined molecular weight, pI and species searches select 12612 entries.
Pre searches select 12612 entries.

Data Set 1 Results

MS-Fit search selects 462 entries (results displayed for top 5 matches).

Results Summary

	MOWSE Score	#/90(%) Masses Matched	% Cov	% TIC	Mean Err ppm	Data Tol ppm	MS-Digest Index #	Protein MW (Da)/pI	Accession #	Species	Protein Name
1	3804	7 (7)	24.0	7.8	-19.6	18.3		24423/7.0		HUMAN	GTP-binding nuclear protein RAN (TC4) (Ran GTPase) (Androgen receptor-associated protein 24)
2	2783	5 (5)	12.0	5.6	9.84	78.0		77888/8.7		HUMAN	Zinc finger protein 7 (Zinc finger protein KOX4) (Zinc finger protein HF.16)
3	2464	7 (7)	15.0	7.8	18.5	82.1		78092/9.2		HUMAN	RING finger protein 6
4	1936	7 (7)	21.0	7.8	-34.5	75.8		53386/8.6		HUMAN	HYPOTHETICAL PROTEIN DJ845O24.1
5	1226	4 (4)	9.0	4.4	7.28	172		41342/5.9		MOUSE	H-2 CLASS I HISTOCOMPATIBILITY ANTIGEN, D-P ALPHA CHAIN PRECURSOR (H-2D(P))

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65167	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	x	.	.	.	.

Detailed Results

1. 7/90 matches (7%).
Acc. #: Species: HUMAN Name: GTP-binding nuclear protein RAN (TC4) (Ran GTPase) (Androgen receptor-associated protein 24)
Index: MW: 24423 Da pI: 7.0

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
1214.5687	1214.6057	-31		143	152	0	(K) (S)
1244.6189	1244.6275	-6.9	AcetN	1	12	0	(-)AAQGEPQVQFK(L)
1294.5711	1294.6068	-28		61	71	0	(K) (F)
1342.6652	1342.7007	-26		142	152	1	(K) (S)
1689.8355	1689.8600	-15		57	71	1	(R) (F)
1784.8940	1784.9124	-10		153	166	0	(K) (K)
1912.9669	1913.0074	-21		153	167	1	(K) (L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 24% (53/216AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 5/90 matches (5%).
Acc. #: Species: HUMAN Name: Zinc finger protein 7 (Zinc finger protein KOX4) (Zinc finger protein HF.16)
Index: MW: 77888 Da pI: 8.7

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1266.6153 1266.6152 0.068 162 172 1 (K) (E)
2083.0216 2083.0936 -35 207 224 1 (R) (C)
2211.0669 2211.0756 -3.9 143 161 0 (K) (T)
2225.0665 2225.0304 16 606 624 0 (R) (K)
2233.0819 2232.9225 71 240 257 0 (K) (V)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 12% (85/686AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 7/90 matches (7%).
Acc. #: Species: HUMAN Name: RING finger protein 6
Index: MW: 78092 Da pI: 9.2

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1288.6170 1288.6722 -43 287 297 1 (R) (L)
1689.8355 1689.8673 -19 221 236 1 (R) (N)
1747.9486 1747.8224 72 120 135 1 (R) (A)
1784.8940 1784.8581 20 271 286 1 (R) (S)
1806.9107 1806.8299 45 588 602 0 (R) (I)
1912.9669 1912.9625 2.3 631 646 1 (K) (Q)
2211.0669 2210.9518 52 37 53 0 (R) (L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 15% (107/685AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 7/90 matches (7%).
Acc. #: Species: HUMAN Name: HYPOTHETICAL PROTEIN DJ845O24.1
Index: MW: 53386 Da pI: 8.6

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
955.4334 955.4671 -35 1Met-ox 141 148 0 (R)MGEHQPLK(V)
1046.4755 1046.5747 -95 282 289 0 (K) (Y)
1244.6189 1244.6315 -10 236 245 0 (R) (E)
1747.9486 1747.9271 12 202 215 1 (R) (C)
2083.0216 2083.1698 -71 1 19 1 (-) (N)
2211.0669 2211.0790 -5.5 309 326 1 (R) (L)
2501.2366 2501.3292 -37 400 422 1 (R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 21% (100/459AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 4/90 matches (4%).
Acc. #: Species: MOUSE Name: H-2 CLASS I HISTOCOMPATIBILITY ANTIGEN, D-P ALPHA CHAIN PRECURSOR (H-2D(P))
Index: MW: 41342 Da pI: 5.9

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
954.4573 954.5022 -47 208 215 0 (K) (S)
1065.3526 1065.4424 -84 121 129 0 (R) (L)
2283.1776 2282.9664 92 72 89 0 (R) (D)
2299.1175 2298.9614 68 1Met-ox 72 89 0 (R)WMEQEGPEYWEQETQNAK(D)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 9% (35/368AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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