MS-Fit Search Results



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Parameters

Database searched: SwissProt.01.22.2003
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): 40-01
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 121920
Molecular weight search (1000 - 100000 Da) selects 115277 entries.
Full pI range: 121920 entries.
Species search ( HUMAN MOUSE ) selects 14893 entries.
Combined molecular weight, pI and species searches select 13163 entries.
Pre searches select 13163 entries.

Data Set 1 Results

MS-Fit search selects 689 entries (results displayed for top 5 matches).

Results Summary

MOWSE
Score
#/80(%)
Masses
Matched
%
Cov
%
TIC
Mean
Err
ppm
Data
Tol
ppm
MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
13.866e+00611 (13)25.013.824.658.8 66018/8.2HUMANKeratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
25.740e+0048 (10)20.010.016.866.7 61988/5.1HUMANKeratin, type I cytoskeletal 9 (Cytokeratin 9) (K9) (CK 9)
31.430e+0048 (10)14.010.0-7.0450.9 73004/7.2HUMANMannosyl-oligosaccharide 1,2-alpha-mannosidase IB (Processing alpha-1,2-mannosidase IB) (Alpha-1,2-mannosidase IB) (Mannosidase alpha class 1A member 2)
41.005e+0047 (8)14.08.8-19.961.2 69900/6.3HUMANBaculoviral IAP repeat-containing protein 3 (Inhibitor of apoptosis protein 2) (HIAP2) (HIAP-2) (C-IAP1) (TNFR2-TRAF signaling complex protein 2) (IAP homolog B)
590379 (11)20.011.3-2.9374.1 73244/6.7HUMANDEAD-box protein 3 (Helicase-like protein 2) (HLP2) (DEAD-box, X isoform)
48753...................x..........x.x...x...x.x..x.x.....x...............x..x.......
104932.........................xx...x.......xx.........xx........................x....
98649..x......................x..x..........x..........x..x..x.....................x.
19304................x......x.......x.........................xx.............xx......
3614..............x...............xx.x...x........x....x....x.................x.....



Detailed Results

1. 11/80 matches (13%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
Index: MW: 66018 Da pI: 8.2
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1016.44181016.5013-583563640(K) (S)
1157.53511157.5149171Met-ox 4644720(R)DYQELMNTK(L)
1179.64941179.6010413773860(K) (H)
1277.71161277.71050.834734830(K) (T)
1383.74361383.6909381861970(K) (V)
1475.81001475.7494412122230(K) (T)
1475.81001475.7858162002110(R) (W)
1688.04331687.97074366821(R) (G)
1716.92311716.8517424184320(K) (G)
1994.05751993.9772402242390(R) (R)
2300.27392300.1985333443641(R) (S)
2384.05072383.9524415195490(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 25% (166/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 8/80 matches (10%).
Acc. #: Species: HUMAN Name: Keratin, type I cytoskeletal 9 (Cytokeratin 9) (K9) (CK 9)
Index: MW: 61988 Da pI: 5.1
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1060.55431060.5639-9.02242320(K) (M)
1065.50081065.49990.861541620(K) (L)
1157.53511157.5915-492502600(R) (Q)
1307.72991307.6782402402491(R) (Q)
1350.74181350.6878404724861(K) (G)
1791.81521791.7283494905120(R) (G)
1838.01371837.9660263743890(R) (K)
2705.26282705.16173763940(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 20% (125/622AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 8/80 matches (10%).
Acc. #: Species: HUMAN Name: Mannosyl-oligosaccharide 1,2-alpha-mannosidase IB (Processing alpha-1,2-mannosidase IB) (Alpha-1,2-mannosidase IB) (Mannosidase alpha class 1A member 2)
Index: MW: 73004 Da pI: 7.2
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
864.4720864.4579165675750(R) (D)
1060.55431060.5639-9.01351431(R) (V)
1107.56021107.5839-214404490(R) (N)
1350.74181350.7170184384491(K) (N)
1838.01371837.97002466820(R) (G)
1994.05751994.0711-6.865821(K) (G)
2083.07952083.1414-302983160(K) (S)
2921.40892921.5485-481521781(K) (E)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 14% (94/641AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 7/80 matches (8%).
Acc. #: Species: HUMAN Name: Baculoviral IAP repeat-containing protein 3 (Inhibitor of apoptosis protein 2) (HIAP2) (HIAP-2) (C-IAP1) (TNFR2-TRAF signaling complex protein 2) (IAP homolog B)
Index: MW: 69900 Da pI: 6.3
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
963.4399963.4747-361651730(R) (T)
1038.55611038.54728.64174250(K) (T)
1165.62461165.6693-38pyroGlu 4985071(K)QKTQIPLQAR(E)
2105.01732105.0879-345475641(K) (L)
2193.98152194.0214-182072261(R) (L)
2384.05072384.1808-55pyroGlu 1051260(K)QLYPSCSFIQNLVSASLGSTSK(N)
2401.28662401.2073331051260(K) (N)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 14% (88/618AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 9/80 matches (11%).
Acc. #: Species: HUMAN Name: DEAD-box protein 3 (Helicase-like protein 2) (HLP2) (DEAD-box, X isoform)
Index: MW: 73244 Da pI: 6.7
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
940.3561940.4012-4856640(K) (D)
1157.53511157.5876-453643730(R) (G)
1165.62461165.6594-3038461(R) (E)
1232.62431232.55485665751(K) (S)
1232.62431232.5812355936031(R) (Q)
1300.61371300.6398-204794881(R) (S)
1707.83731707.8964-353123261(R) (L)
1941.01061940.9792163363511(K) (M)
2083.07952083.0249265125280(K) (I)
2662.46792662.4278152312551(K) (E)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 20% (133/662AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):


MS-Fit in ProteinProspector 4.0.4
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