MS-Fit Search Results

Parameters

Database searched: SwissProt.01.22.2003
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): 40-02
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 121920
Molecular weight search (10000 - 150000 Da) selects 108462 entries.
Full pI range: 121920 entries.
Species search ( HUMAN MOUSE ) selects 14893 entries.
Combined molecular weight, pI and species searches select 13842 entries.
Pre searches select 13842 entries.

Data Set 1 Results

MS-Fit search selects 1741 entries (results displayed for top 5 matches).

Results Summary

	MOWSE Score	#/77(%) Masses Matched	% Cov	% TIC	Mean Err ppm	Data Tol ppm	Protein MW (Da)/pI	Species	Protein Name
1	5.300e+008	18 (23)	37.0	23.4	-33.6	49.6	66018/8.2	HUMAN	Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
2	3.149e+006	17 (22)	26.0	22.1	-41.6	50.7	59519/5.1	HUMAN	Keratin, type I cytoskeletal 10 (Cytokeratin 10) (K10) (CK 10)
3	1.402e+006	12 (15)	26.0	15.6	-30.3	71.1	61988/5.1	HUMAN	Keratin, type I cytoskeletal 9 (Cytokeratin 9) (K9) (CK 9)
4	1.259e+006	18 (23)	24.0	23.4	-38.3	44.4	95339/6.4	HUMAN	Elongation factor 2 (EF-2)
5	6.921e+005	17 (22)	24.0	22.1	-34.4	41.2	95315/6.4	MOUSE	Elongation factor 2 (EF-2)

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Detailed Results

1. 18/77 matches (23%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
Index: MW: 66018 Da pI: 8.2

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
973.4647	973.5318	-69		396	403	0	(K) (V)
1033.4637	1033.5166	-51		484	492	0	(R) (M)
1141.4691	1141.5199	-45		464	472	0	(R) (L)
1157.5117	1157.5149	-2.7	1Met-ox	464	472	0	(R)DYQELMNTK(L)
1179.5396	1179.6010	-52		377	386	0	(K) (H)
1265.5728	1265.6378	-51		278	288	0	(R) (K)
1277.6599	1277.6337	20		247	257	1	(K) (N)
1277.6599	1277.7105	-40		473	483	0	(K) (T)
1300.5103	1300.5302	-15		258	267	0	(K) (N)
1302.6048	1302.7018	-74		393	403	1	(R) (V)
1316.4888	1316.5251	-28	1Met-ox	258	267	0	(K)NMQDMVEDYR(N)
1340.6050	1340.6698	-48		365	376	1	(K) (Y)
1357.6525	1357.6963	-32		444	455	0	(K) (E)
1383.6189	1383.6909	-52		186	197	0	(K) (V)
1475.7074	1475.7494	-28		212	223	0	(K) (T)
1475.7074	1475.7858	-53		200	211	0	(R) (W)
1716.8108	1716.8517	-24		418	432	0	(K) (G)
1993.9237	1993.9772	-27		224	239	0	(R) (R)
2383.9405	2383.9524	-5.0		519	549	0	(R) (G)
3312.3257	3312.3087	5.1		550	588	0	(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 37% (243/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 17/77 matches (22%).
Acc. #: Species: HUMAN Name: Keratin, type I cytoskeletal 10 (Cytokeratin 10) (K10) (CK 10)
Index: MW: 59519 Da pI: 5.1

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
995.4555 995.5202 -65 178 184 1 (K) (H)
1060.5051 1060.4733 30 1Met-ox 285 292 1 (K)KNHEEEMK(D)
1060.5051 1060.5097 -4.4 1Met-ox 1 9 1 (-)MSVRYSSSK(H)
1090.4523 1090.5315 -73 148 156 0 (K) (L)
1109.4352 1109.4904 -50 335 343 0 (K) (S)
1165.5158 1165.5853 -60 442 450 0 (R) (S)
1201.5681 1201.6177 -41 246 256 0 (R) (R)
1234.6063 1234.6796 -59 236 245 1 (R) (Q)
1262.5540 1262.5977 -35 451 464 0 (R) (G)
1300.5103 1300.5956 -66 286 295 1 (K) (N)
1340.6050 1340.6923 -65 pyroGlu 246 257 1 (R)QSVEADINGLRR(V)
1357.6525 1357.7188 -49 246 257 1 (R) (V)
1365.5779 1365.6399 -45 323 333 0 (R) (K)
1390.6266 1390.6814 -39 387 399 0 (K) (Y)
1434.7026 1434.7705 -47 440 450 1 (K) (S)
1493.6756 1493.7348 -40 323 334 1 (R) (D)
1707.7282 1707.7727 -26 41 59 0 (K) (G)
2367.2271 2367.2632 -15 208 228 0 (K) (L)

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
995.4555	995.5202	-65		178	184	1	(K) (H)
1060.5051	1060.4733	30	1Met-ox	285	292	1	(K)KNHEEEMK(D)
1060.5051	1060.5097	-4.4	1Met-ox	1	9	1	(-)MSVRYSSSK(H)
1090.4523	1090.5315	-73		148	156	0	(K) (L)
1109.4352	1109.4904	-50		335	343	0	(K) (S)
1165.5158	1165.5853	-60		442	450	0	(R) (S)
1201.5681	1201.6177	-41		246	256	0	(R) (R)
1234.6063	1234.6796	-59		236	245	1	(R) (Q)
1262.5540	1262.5977	-35		451	464	0	(R) (G)
1300.5103	1300.5956	-66		286	295	1	(K) (N)
1340.6050	1340.6923	-65	pyroGlu	246	257	1	(R)QSVEADINGLRR(V)
1357.6525	1357.7188	-49		246	257	1	(R) (V)
1365.5779	1365.6399	-45		323	333	0	(R) (K)
1390.6266	1390.6814	-39		387	399	0	(K) (Y)
1434.7026	1434.7705	-47		440	450	1	(K) (S)
1493.6756	1493.7348	-40		323	334	1	(R) (D)
1707.7282	1707.7727	-26		41	59	0	(K) (G)
2367.2271	2367.2632	-15		208	228	0	(K) (L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 26% (157/593AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 12/77 matches (15%).
Acc. #: Species: HUMAN Name: Keratin, type I cytoskeletal 9 (Cytokeratin 9) (K9) (CK 9)
Index: MW: 61988 Da pI: 5.1

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
897.3633 897.4140 -56 233 239 0 (R) (I)
1060.5051 1060.5639 -55 224 232 0 (K) (M)
1065.4422 1065.4999 -54 154 162 0 (K) (L)
1157.5117 1157.5915 -69 250 260 0 (R) (Q)
1277.6599 1277.5875 57 29 40 1 (R) (G)
1307.6158 1307.6782 -48 240 249 1 (R) (Q)
1323.5925 1323.6731 -61 1Met-ox 240 249 1 (R)IKFEMEQNLR(Q)
1791.6975 1791.7283 -17 490 512 0 (R) (G)
1837.9148 1837.9660 -28 374 389 0 (R) (K)
1851.8800 1851.9275 -26 321 335 1 (K) (N)
2510.1310 2510.1323 -0.51 449 471 0 (K) (I)
2705.1471 2705.1617 -5.4 63 94 0 (R) (G)

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
897.3633	897.4140	-56		233	239	0	(R) (I)
1060.5051	1060.5639	-55		224	232	0	(K) (M)
1065.4422	1065.4999	-54		154	162	0	(K) (L)
1157.5117	1157.5915	-69		250	260	0	(R) (Q)
1277.6599	1277.5875	57		29	40	1	(R) (G)
1307.6158	1307.6782	-48		240	249	1	(R) (Q)
1323.5925	1323.6731	-61	1Met-ox	240	249	1	(R)IKFEMEQNLR(Q)
1791.6975	1791.7283	-17		490	512	0	(R) (G)
1837.9148	1837.9660	-28		374	389	0	(R) (K)
1851.8800	1851.9275	-26		321	335	1	(K) (N)
2510.1310	2510.1323	-0.51		449	471	0	(K) (I)
2705.1471	2705.1617	-5.4		63	94	0	(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 26% (167/622AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 18/77 matches (23%).
Acc. #: Species: HUMAN Name: Elongation factor 2 (EF-2)
Index: MW: 95339 Da pI: 6.4

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
890.4488 890.5100 -69 499 506 0 (K) (V)
896.4203 896.4221 -2.1 1Met-ox 252 258 1 (K)KVEDMMK(K)
896.4203 896.4221 -2.1 1Met-ox 253 259 1 (K)VEDMMKK(L)
969.4814 969.5482 -69 717 726 0 (R) (R)
973.4647 973.5008 -37 408 415 1 (K) (V)
1013.4445 1013.5016 -56 240 249 0 (K) (A)
1100.4628 1100.5450 -75 1Met-ox 300 308 0 (K)VFDAIMNFK(K)
1138.4689 1138.5169 -42 639 647 0 (K) (K)
1274.6438 1274.7109 -53 440 449 0 (K) (T)
1307.6158 1307.6530 -29 21 32 0 (R) (S)
1323.5925 1323.6479 -42 1Met-ox 21 32 0 (R)NMSVIAHVDHGK(S)
1392.6597 1392.7309 -51 728 739 0 (R) (L)
1475.7074 1475.7901 -56 151 162 1 (R) (A)
1631.6884 1631.7600 -44 1Met-ox 482 495 0 (K)TGTITTFEHAHNMR(V)
1799.8572 1799.8968 -22 786 801 0 (K) (S)
1977.9066 1977.9744 -34 1Met-ox 768 785 0 (R)GHVFEESQVAGTPMFVVK(A)
2037.8830 2037.9149 -16 1Met-ox 369 386 0 (R)CELLYEGPPDDEAAMGIK(S)
2143.0358 2143.0784 -20 606 625 1 (K) (Q)
2220.1321 2220.1552 -10 163 180 0 (R) (I)

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
890.4488	890.5100	-69		499	506	0	(K) (V)
896.4203	896.4221	-2.1	1Met-ox	252	258	1	(K)KVEDMMK(K)
896.4203	896.4221	-2.1	1Met-ox	253	259	1	(K)VEDMMKK(L)
969.4814	969.5482	-69		717	726	0	(R) (R)
973.4647	973.5008	-37		408	415	1	(K) (V)
1013.4445	1013.5016	-56		240	249	0	(K) (A)
1100.4628	1100.5450	-75	1Met-ox	300	308	0	(K)VFDAIMNFK(K)
1138.4689	1138.5169	-42		639	647	0	(K) (K)
1274.6438	1274.7109	-53		440	449	0	(K) (T)
1307.6158	1307.6530	-29		21	32	0	(R) (S)
1323.5925	1323.6479	-42	1Met-ox	21	32	0	(R)NMSVIAHVDHGK(S)
1392.6597	1392.7309	-51		728	739	0	(R) (L)
1475.7074	1475.7901	-56		151	162	1	(R) (A)
1631.6884	1631.7600	-44	1Met-ox	482	495	0	(K)TGTITTFEHAHNMR(V)
1799.8572	1799.8968	-22		786	801	0	(K) (S)
1977.9066	1977.9744	-34	1Met-ox	768	785	0	(R)GHVFEESQVAGTPMFVVK(A)
2037.8830	2037.9149	-16	1Met-ox	369	386	0	(R)CELLYEGPPDDEAAMGIK(S)
2143.0358	2143.0784	-20		606	625	1	(K) (Q)
2220.1321	2220.1552	-10		163	180	0	(R) (I)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 24% (212/858AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 17/77 matches (22%).
Acc. #: Species: MOUSE Name: Elongation factor 2 (EF-2)
Index: MW: 95315 Da pI: 6.4

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
890.4488 890.5100 -69 499 506 0 (K) (V)
896.4203 896.4221 -2.1 1Met-ox 252 258 1 (K)KVEDMMK(K)
896.4203 896.4221 -2.1 1Met-ox 253 259 1 (K)VEDMMKK(L)
969.4814 969.5482 -69 717 726 0 (R) (R)
973.4647 973.5008 -37 408 415 1 (K) (V)
1138.4689 1138.5169 -42 639 647 0 (K) (K)
1274.6438 1274.7109 -53 440 449 0 (K) (T)
1307.6158 1307.6530 -29 21 32 0 (R) (S)
1323.5925 1323.6479 -42 1Met-ox 21 32 0 (R)NMSVIAHVDHGK(S)
1392.6597 1392.7309 -51 728 739 0 (R) (L)
1434.7026 1434.7341 -22 236 249 1 (K) (A)
1475.7074 1475.7901 -56 151 162 1 (R) (A)
1631.6884 1631.7600 -44 1Met-ox 482 495 0 (K)TGTITTFEHAHNMR(V)
1799.8572 1799.8968 -22 786 801 0 (K) (S)
1977.9066 1977.9744 -34 1Met-ox 768 785 0 (R)GHVFEESQVAGTPMFVVK(A)
2037.8830 2037.9149 -16 1Met-ox 369 386 0 (R)CELLYEGPPDDEAAMGIK(S)
2143.0358 2143.0784 -20 606 625 1 (K) (Q)
2220.1321 2220.1552 -10 163 180 0 (R) (I)

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
890.4488	890.5100	-69		499	506	0	(K) (V)
896.4203	896.4221	-2.1	1Met-ox	252	258	1	(K)KVEDMMK(K)
896.4203	896.4221	-2.1	1Met-ox	253	259	1	(K)VEDMMKK(L)
969.4814	969.5482	-69		717	726	0	(R) (R)
973.4647	973.5008	-37		408	415	1	(K) (V)
1138.4689	1138.5169	-42		639	647	0	(K) (K)
1274.6438	1274.7109	-53		440	449	0	(K) (T)
1307.6158	1307.6530	-29		21	32	0	(R) (S)
1323.5925	1323.6479	-42	1Met-ox	21	32	0	(R)NMSVIAHVDHGK(S)
1392.6597	1392.7309	-51		728	739	0	(R) (L)
1434.7026	1434.7341	-22		236	249	1	(K) (A)
1475.7074	1475.7901	-56		151	162	1	(R) (A)
1631.6884	1631.7600	-44	1Met-ox	482	495	0	(K)TGTITTFEHAHNMR(V)
1799.8572	1799.8968	-22		786	801	0	(K) (S)
1977.9066	1977.9744	-34	1Met-ox	768	785	0	(R)GHVFEESQVAGTPMFVVK(A)
2037.8830	2037.9149	-16	1Met-ox	369	386	0	(R)CELLYEGPPDDEAAMGIK(S)
2143.0358	2143.0784	-20		606	625	1	(K) (Q)
2220.1321	2220.1552	-10		163	180	0	(R) (I)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 24% (207/858AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):