MS-Fit Search Results


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Parameters

Database searched: SwissProt.01.22.2003
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): 40-04
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 121920
Molecular weight search (1000 - 100000 Da) selects 115277 entries.
Full pI range: 121920 entries.
Species search ( HUMAN MOUSE ) selects 14893 entries.
Combined molecular weight, pI and species searches select 13163 entries.
Pre searches select 13163 entries.

Data Set 1 Results

MS-Fit search selects 1951 entries (results displayed for top 5 matches).

Results Summary

MOWSE
Score		#/103(%)
Masses
Matched 		%
Cov		%
TIC		Mean
Err
ppm		Data
Tol
ppm		MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
11.980e+01641 (39)41.039.87.6428.9 95339/6.4HUMANElongation factor 2 (EF-2)
23.027e+01437 (35)39.035.910.531.1 95315/6.4MOUSEElongation factor 2 (EF-2)
31.101e+00514 (13)15.013.6-13.139.5 98146/7.0MOUSEDynamin 2 (Dynamin UDNM)
43.365e+00413 (12)14.012.6-11.839.8 98019/7.0HUMANDynamin 2
5812810 (9)14.09.712.043.4 78791/9.6HUMANMyosin Id
60102.....x..xx.x...x.x.....x.x..xx......xxxx.x..xx..........xxxx..xx..x.......xx..xxxx.xxxx.x.x.....xx.xx.x
40844.....x..xx.x...x.......x.x...x.......xx..x..xx..........xx.xx.xx..x.......xx..xxxx.xxxx.x.x.....xx.xx.x
28747........x...............x...x...............xx....x..xx......x......x.x.x......................x...x...
119902........x...............x...x...............xx....x..xx......x......x.x.x......................x.......
97956.....x...........x..............................x......xx....x..............xxx....................x...


Detailed Results

1. 41/103 matches (39%).
Acc. #: Species: HUMAN Name: Elongation factor 2 (EF-2)
Index: MW: 95339 Da pI: 6.4
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
890.5204890.5100124995060(K) (V)
911.4331911.42637.42652720(R) (F)
912.4169912.4171-0.172Met-ox 2522581(K)KVEDMMK(K)
912.4169912.4171-0.172Met-ox 2532591(K)VEDMMKK(L)
922.4734922.4634115735800(K) (E)
969.5574969.54829.67177260(R) (R)
1013.51051013.50168.82402490(K) (A)
1063.57031063.5788-8.06686760(K) (D)
1071.52611071.52194.02Met-ox 3924000(K)GPLMMYISK(M)
1084.55521084.55014.73003080(K) (K)
1085.53081085.497830pyroGlu 1Met-ox 2272350(K)QFAEMYVAK(F)
1100.55101100.54505.51Met-ox 3003080(K)VFDAIMNFK(K)
1102.54041102.5243151Met-ox 2272350(K)QFAEMYVAK(F)
1105.58171105.6113-272Met-ox 1511590(R)IKPVLMMNK(M)
1107.63581107.6414-5.04164260(R) (V)
1125.59731125.6493-467177271(R) (C)
1137.58491137.58252.133420(K) (A)
1138.54561138.5169256396470(K) (K)
1138.54561138.5744-252732831(K) (K)
1221.62601221.6302-3.41Met-ox 4294391(R)IMGPNYTPGKK(E)
1222.56001222.5196336896980(K) (G)
1228.64821228.64006.61Met-ox 3003091(K)VFDAIMNFKK(E)
1238.53571238.5145171Met-ox 6896980(K)EGALCEENMR(G)
1272.71211272.70247.643551(K) (F)
1274.72621274.7109124404490(K) (T)
1308.67101308.65889.36776880(K) (E)
1392.75571392.7309187287390(R) (L)
1402.81651402.80587.64394491(K) (T)
1475.78101475.7901-6.11511621(R) (A)
1508.83981508.8187142882990(R) (V)
1543.79711543.7756146066190(K) (G)
1548.85371548.8321147277391(R) (L)
1581.81031581.79479.95605721(K) (S)
1608.79031608.77917.05815940(R) (S)
1615.78731615.7651144824950(K) (V)
1631.78921631.7600181Met-ox 4824950(K)TGTITTFEHAHNMR(V)
1742.86401742.8390146346471(R) (K)
1799.91291799.89689.07868010(K) (S)
1978.00871977.9744171Met-ox 7687850(R)GHVFEESQVAGTPMFVVK(A)
2037.96722037.9149261Met-ox 3693860(R)CELLYEGPPDDEAAMGIK(S)
2134.10772134.0755151Met-ox 7677851(K)RGHVFEESQVAGTPMFVVK(A)
2143.11442143.0784176066251(K) (Q)
2220.18952220.1552151631800(R) (I)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 41% (360/858AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 37/103 matches (35%).
Acc. #: Species: MOUSE Name: Elongation factor 2 (EF-2)
Index: MW: 95315 Da pI: 6.4
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
890.5204890.5100124995060(K) (V)
911.4331911.42637.42652720(R) (F)
912.4169912.4171-0.172Met-ox 2522581(K)KVEDMMK(K)
912.4169912.4171-0.172Met-ox 2532591(K)VEDMMKK(L)
922.4734922.4634115735800(K) (E)
969.5574969.54829.67177260(R) (R)
1063.57031063.5788-8.06686760(K) (D)
1071.52611071.52194.02Met-ox 3924000(K)GPLMMYISK(M)
1085.53081085.497830pyroGlu 1Met-ox 2272350(K)QFAEMYVAK(F)
1102.54041102.5243151Met-ox 2272350(K)QFAEMYVAK(F)
1105.58171105.6113-272Met-ox 1511590(R)IKPVLMMNK(M)
1125.59731125.6493-467177271(R) (C)
1137.58491137.5540272732831(K) (K)
1137.58491137.58252.133420(K) (A)
1138.54561138.5169256396470(K) (K)
1221.62601221.6302-3.41Met-ox 4294391(R)IMGPNYTPGKK(E)
1222.56001222.5196336896980(K) (G)
1238.53571238.5145171Met-ox 6896980(K)EGALCEENMR(G)
1256.70591256.6462481Met-ox 3003091(K)VFDAIMNFRK(E)
1272.71211272.70247.643551(K) (F)
1274.72621274.7109124404490(K) (T)
1308.67101308.65889.36776880(K) (E)
1392.75571392.7309187287390(R) (L)
1402.81651402.80587.64394491(K) (T)
1475.78101475.7901-6.11511621(R) (A)
1508.83981508.8187142882990(R) (V)
1543.79711543.7756146066190(K) (G)
1548.85371548.8321147277391(R) (L)
1581.81031581.79479.95605721(K) (S)
1608.79031608.77917.05815940(R) (S)
1615.78731615.7651144824950(K) (V)
1631.78921631.7600181Met-ox 4824950(K)TGTITTFEHAHNMR(V)
1742.86401742.8390146346471(R) (K)
1799.91291799.89689.07868010(K) (S)
1978.00871977.9744171Met-ox 7687850(R)GHVFEESQVAGTPMFVVK(A)
2037.96722037.9149261Met-ox 3693860(R)CELLYEGPPDDEAAMGIK(S)
2134.10772134.0755151Met-ox 7677851(K)RGHVFEESQVAGTPMFVVK(A)
2143.11442143.0784176066251(K) (Q)
2220.18952220.1552151631800(R) (I)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 39% (339/858AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 14/103 matches (13%).
Acc. #: Species: MOUSE Name: Dynamin 2 (Dynamin UDNM)
Index: MW: 98146 Da pI: 7.0
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
911.4331911.4773-492722790(R) (T)
1065.61771065.6421-231141230(K) (V)
1084.55521084.6016-433623691(R) (F)
1137.58491137.58450.352582660(K) (H)
1138.54561138.5931-421Met-ox 1581660(K)DMILQFISR(E)
1154.53701154.5516-135745831(R) (H)
1170.53811170.5465-7.21Met-ox 5745831(R)DVEKGFMSNK(H)
1186.68911186.6948-4.98388490(R) (R)
1265.67371265.6795-4.62572661(R) (H)
1322.77851322.7796-0.865115220(R) (R)
1337.73121337.72187.06186291(K) (D)
1337.73121337.72901.72802900(K) (E)
1361.72041361.6966175845940(K) (N)
1915.96921915.9808-6.01Met-ox 6736881(R)DLMPKTIMHLMINNTK(A)
2134.10772134.1733-312802971(K) (S)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 15% (136/870AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 13/103 matches (12%).
Acc. #: Species: HUMAN Name: Dynamin 2
Index: MW: 98019 Da pI: 7.0
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
911.4331911.4773-492722790(R) (T)
1065.61771065.6421-231141230(K) (V)
1084.55521084.6016-433623691(R) (F)
1137.58491137.58450.352582660(K) (H)
1138.54561138.5931-421Met-ox 1581660(K)DMILQFISR(E)
1154.53701154.5516-135745831(R) (H)
1170.53811170.5465-7.21Met-ox 5745831(R)DVEKGFMSNK(H)
1186.68911186.6948-4.98388490(R) (R)
1265.67371265.6795-4.62572661(R) (H)
1322.77851322.7796-0.865115220(R) (R)
1337.73121337.72187.06186291(K) (D)
1337.73121337.72901.72802900(K) (E)
1361.72041361.6966175845940(K) (N)
1915.96921915.9808-6.01Met-ox 6736881(R)DLMPKTIMHLMINNTK(A)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 14% (129/870AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 10/103 matches (9%).
Acc. #: Species: HUMAN Name: Myosin Id
Index: MW: 78791 Da pI: 9.6
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
890.5204890.51990.632202270(K) (R)
1013.51051013.4944161932000(K) (R)
1148.59271148.5774131Met-ox 5515591(R)HLYKMDPTK(Q)
1187.61381187.60616.56536620(R) (N)
1221.62601221.6380-9.92983061(R) (Y)
1265.67371265.6564142022120(R) (N)
1439.75201439.6928415225320(K) (K)
1443.82351443.7596445825931(K) (D)
1475.78101475.8222-283513621(R) (A)
2134.10772134.0603226456621(K) (N)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 14% (98/674AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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