MS-Fit Search Results


.

Parameters

Database searched: SwissProt.01.22.2003
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): 40-6
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 121920
Molecular weight search (10000 - 150000 Da) selects 108462 entries.
Full pI range: 121920 entries.
Species search ( HUMAN MOUSE ) selects 14893 entries.
Combined molecular weight, pI and species searches select 13842 entries.
Pre searches select 13842 entries.

Data Set 1 Results

MS-Fit search selects 1329 entries (results displayed for top 5 matches).

Results Summary

MOWSE
Score		#/75(%)
Masses
Matched 		%
Cov		%
TIC		Mean
Err
ppm		Data
Tol
ppm		MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
12.588e+01644 (58)48.058.727.421.5 101560/6.9HUMANC-1-tetrahydrofolate synthase, cytoplasmic (C1-THF synthase) [Includes: Methylenetetrahydrofolate dehydrogenase ; Methenyltetrahydrofolate cyclohydrolase ; Formyltetrahydrofolate synthetase ]
236979 (12)13.012.029.553.5 105339/8.0MOUSERetinoblastoma-associated protein (PP105) (RB)
3368411 (14)12.014.712.359.3 119521/8.9HUMANBromodomain-containing protein 1 (BR140-like protein)
421038 (10)11.010.717.446.8 78339/8.6HUMANLactotransferrin precursor (Lactoferrin) [Contains: Lactoferroxin A; Lactoferroxin B; Lactoferroxin C]
519119 (12)15.012.021.332.9 59451/8.3MOUSEKeratin, type II cytoskeletal 6 (Cytokeratin 6) (CK 6) (K6 keratin)
103103..x.xx..xx.xxx.xx...xxxxxxxxx.xxxxxx..x..xx.xxx....xx...x...x.xxx.x.xxx..xx
78818.....x..............x.............xx.x....x....x..................x..x.....
70508...........x..x..x..xx.x....x......x...........x..............x...x........
96645........x........................x..x.....xx......x............xx..........
54586...............x....x......x.x.....x...xx..x....x..........................


Detailed Results

1. 44/75 matches (58%).
Acc. #: Species: HUMAN Name: C-1-tetrahydrofolate synthase, cytoplasmic (C1-THF synthase) [Includes: Methylenetetrahydrofolate dehydrogenase ; Methenyltetrahydrofolate cyclohydrolase ; Formyltetrahydrofolate synthetase ]
Index: MW: 101560 Da pI: 6.9
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
878.4882878.4736174894950(R) (R)
899.5144899.4951221581660(K) (H)
900.5593900.55188.33553620(K) (L)
935.5729935.5791-6.667741(K) (T)
940.5755940.5580194794870(R) (R)
969.5845969.5733128208271(K) (I)
973.4885973.4743157767840(R) (C)
983.6194983.60022038460(R) (D)
1030.55901030.53222629370(K) (L)
1034.60151034.5747264894961(R) (L)
1034.60151034.5747264884951(R) (R)
1096.67101096.6591114794881(R) (F)
1097.67131097.6431267948040(K) (A)
1142.60891142.584621pyroGlu 4114210(R)QPSQGPTFGIK(G)
1159.63131159.6111174114210(R) (G)
1169.73661169.7047278798890(K) (D)
1185.70121185.6843145435531(R) (G)
1199.66771199.6272343143240(K) (S)
1231.67171231.6323324644730(R) (A)
1248.70251248.6701265335421(R) (K)
1348.68471348.639434pyroGlu 1Met-ox 8558660(K)QGFGNLPICMAK(T)
1365.71241365.6659341Met-ox 8558660(K)QGFGNLPICMAK(T)
1378.71371378.6702327617720(K) (L)
1390.74051390.7079238678780(K) (G)
1448.80721448.7637307227330(K) (G)
1450.77661450.7178412522641(K) (A)
1486.79781486.7542295215320(R) (V)
1504.75961504.7131315055170(K) (F)
1507.87021507.8347241Met-ox 7477600(R)MFGIPVVVAVNAFK(T)
1548.87341548.8208341Met-ox 7067210(K)MHGGGPTVTAGLPLPK(A)
1553.95741553.9168268798921(K) (A)
1564.78231564.7140441381500(R) (G)
1630.96131630.9056343713860(K) (S)
1651.92031651.8695318058190(R) (L)
1727.00501726.9526301511661(K) (H)
1784.04921783.9893346877020(R) (A)
1808.98331808.9170373373520(R) (A)
1811.98461811.9180378338480(K) (A)
1842.04321841.98743022371(K) (L)
2045.10612045.0403325005171(R) (F)
2117.20582117.1164421Met-ox 5966161(K)KGEPVSAEDLGVSGALTVLMK(D)
2138.15892138.0929317858041(K) (A)
2145.21892145.16682438561(R) (L)
2239.21482239.0957531Met-ox 6586790(K)LVGPEGFVVTEAGFGADIGMEK(F)
2503.38742503.2720468338541(K) (Q)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 48% (451/935AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 9/75 matches (12%).
Acc. #: Species: MOUSE Name: Retinoblastoma-associated protein (PP105) (RB)
Index: MW: 105339 Da pI: 8.0
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
900.5593900.5307322232300(K) (E)
1096.67101096.6115542352450(K) (T)
1448.80721448.7282551Met-ox 4464561(R)LYYRVMESMLK(S)
1450.77661450.8130-252352481(K) (R)
1466.81211466.7742268248370(R) (F)
1507.87021507.80084675880(K) (K)
1566.80001566.7434361311441(K) (L)
1566.80001566.7764154574691(K) (L)
2045.10612045.1072-0.527167331(K) (R)
2138.15892138.03675741570(R) (L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 13% (123/921AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 11/75 matches (14%).
Acc. #: Species: HUMAN Name: Bromodomain-containing protein 1 (BR140-like protein)
Index: MW: 119521 Da pI: 8.9
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
969.5845969.5733127917980(R) (K)
1012.54991012.5904-404604680(R) (K)
1045.57641045.5530225735810(R) (D)
1096.67101096.6591114854941(R) (R)
1096.67101096.7206-455355431(R) (K)
1097.67131097.66832.87917991(R) (R)
1159.63131159.62644.399710051(R) (M)
1248.70251248.6337558198310(R) (S)
1450.77661450.7364281Met-ox 3964070(R)RPLNIYGDVEMK(N)
1566.80001566.7581277207320(R) (S)
1808.98331808.9217343643801(K) (K)
2045.10612045.0304371011210(K) (V)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 12% (131/1058AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 8/75 matches (10%).
Acc. #: Species: HUMAN Name: Lactotransferrin precursor (Lactoferrin) [Contains: Lactoferroxin A; Lactoferroxin B; Lactoferroxin C]
Index: MW: 78339 Da pI: 8.6
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
935.5729935.5427322702780(R) (S)
1390.74051390.73315.41211330(K) (S)
1459.73381459.7368-2.03653760(R) (K)
1507.87021507.7909533033161(K) (D)
1518.79911518.8280-191201331(K) (S)
1628.86761628.80663760730(R) (A)
1811.98461811.96968.33063211(K) (D)
1842.04321841.998624941091(K) (T)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 11% (84/711AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 9/75 matches (12%).
Acc. #: Species: MOUSE Name: Keratin, type II cytoskeletal 6 (Cytokeratin 6) (CK 6) (K6 keratin)
Index: MW: 59451 Da pI: 8.3
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1030.55901030.55335.52132210(R) (G)
1096.67101096.6115543803881(R) (K)
1231.67171231.6323321841930(K) (T)
1260.66151260.6258282302390(R) (N)
1450.77661450.7766-0.02515281(R) (S)
1500.81031500.79579.83683791(K) (L)
1502.78501502.7637142302411(R) (Y)
1518.79911518.7586271Met-ox 2302411(R)NMQELVEELRNK(Y)
1578.86661578.8339215235400(R) (Y)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 15% (84/553AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
© Copyright (1995-2002) The Regents of the University of California.