MS-Fit Search Results


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Parameters

Database searched: SwissProt.01.22.2003
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): 40-07
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 121920
Molecular weight search (10000 - 150000 Da) selects 108462 entries.
Full pI range: 121920 entries.
Species search ( HUMAN MOUSE ) selects 14893 entries.
Combined molecular weight, pI and species searches select 13842 entries.
Pre searches select 13842 entries.

Data Set 1 Results

MS-Fit search selects 810 entries (results displayed for top 5 matches).

Results Summary

MOWSE
Score		#/80(%)
Masses
Matched 		%
Cov		%
TIC		Mean
Err
ppm		Data
Tol
ppm		MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
13.728e+00822 (27)34.027.5-16.833.9 90071/6.8HUMANRibonucleoside-diphosphate reductase M1 chain (Ribonucleotide reductase large chain)
21.130e+00515 (18)20.018.8-21.029.6 90220/6.5MOUSERibonucleoside-diphosphate reductase M1 chain (Ribonucleotide reductase large chain)
32.524e+0049 (11)17.011.3-25.331.4 87799/6.7HUMANGlutaminyl-tRNA synthetase (Glutamine--tRNA ligase) (GlnRS)
41.243e+0047 (8)13.08.8-5.3048.2 106058/6.4HUMANGeneral transcription factor II-I repeat domain-containing protein 1 (GTF2I repeat domain containing protein 1) (Muscle TFII-I repeat domain-containing protein 1) (General transcription factor III) (Slow-muscle-fiber enhancer binding protein) (USE B1 binding protein) (MusTRD1/BEN) (Williams-Beuren syndrome chromosome region 11 protein)
572106 (7)14.07.5-1.4727.4 118262/6.6HUMANTyrosine-protein kinase-like 7 precursor (Colon carcinoma kinase-4) (CCK-4)
3503.....................xx...xx.....xxxx..xx.x..x.xx..x.....x.x..x.x.......xx.x....
100630......................x...xx.....xxx...xx....x..x..x.....x.x............xx......
58413..........................x...x.x.............x..x...xx.......x...x.............
92730.......................................x.x.........x.........x....x...x....x....
102394...........................x.....................x............x....x..x......x..


Detailed Results

1. 22/80 matches (27%).
Acc. #: Species: HUMAN Name: Ribonucleoside-diphosphate reductase M1 chain (Ribonucleotide reductase large chain)
Index: MW: 90071 Da pI: 6.8
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1045.50651045.4777281Met-ox 6496560(R)GLWHEEMK(N)
1064.52181064.5740-491311390(R) (D)
1096.59171096.58258.413211(R) (I)
1126.56451126.6009-3271800(K) (I)
1260.63131260.6734-332442560(K) (A)
1285.55531285.6040-381Met-ox 7207290(K)LTSMHFYGWK(Q)
1287.55041287.5686-141401490(R) (T)
1344.71231344.7487-271191301(K) (L)
1383.67461383.6989-181Met-ox 1631730(K)VAERPQHMLMR(V)
1399.65741399.6938-262Met-ox 1631730(K)VAERPQHMLMR(V)
1509.72431509.7589-235175290(R) (Q)
1570.83351570.8706-247417540(R) (E)
1670.72591670.7550-175495630(K) (G)
1693.91571693.9641-296296430(R) (D)
1850.02461850.0652-226286431(R) (D)
2080.12352080.1490-121Met-ox 4985160(R)HRPIGIGVQGLADAFILMR(Y)
2109.04302109.03991.51Met-ox 2572770(R)ATGSYIAGTNGNSNGLVPMLR(V)
2179.09442179.1221-134804971(K) (H)
2206.05582206.0742-8.45305480(K) (E)
2302.11942302.1468-126997190(R) (L)
2333.08642333.1243-161311491(R) (T)
2572.33992572.32595.51741961(R) (W)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 34% (271/792AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 15/80 matches (18%).
Acc. #: Species: MOUSE Name: Ribonucleoside-diphosphate reductase M1 chain (Ribonucleotide reductase large chain)
Index: MW: 90220 Da pI: 6.5
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1064.52181064.5740-491311390(R) (D)
1096.59171096.58258.413211(R) (I)
1126.56451126.6009-3271800(K) (I)
1260.63131260.6734-332442560(K) (A)
1285.55531285.6040-381Met-ox 7207290(K)LTSMHFYGWK(Q)
1287.55041287.5686-141401490(R) (T)
1383.67461383.6989-181Met-ox 1631730(K)VAERPQHMLMR(V)
1399.65741399.6938-262Met-ox 1631730(K)VAERPQHMLMR(V)
1570.83351570.8706-247417540(R) (E)
1693.91571693.9641-296296430(R) (D)
1850.02461850.0652-226286431(R) (D)
2080.12352080.1490-121Met-ox 4985160(R)HRPIGIGVQGLADAFILMR(Y)
2109.04302109.03991.51Met-ox 2572770(R)ATGSYIAGTNGNSNGLVPMLR(V)
2302.11942302.1468-126997190(R) (L)
2333.08642333.1243-161311491(R) (T)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 20% (163/792AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 9/80 matches (11%).
Acc. #: Species: HUMAN Name: Glutaminyl-tRNA synthetase (Glutamine--tRNA ligase) (GlnRS)
Index: MW: 87799 Da pI: 6.7
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1096.59171096.6267-326306390(R) (H)
1144.52481144.5791-472832920(K) (A)
1234.66041234.7159-4551620(K) (L)
1640.77851640.7920-8.3961090(R) (E)
1756.90261756.9539-296026160(K) (T)
1883.99041884.0244-182662821(R) (A)
1980.93431980.9887-271Met-ox 3773921(R)DRPMEESLLLFEAMRK(G)
2179.09442179.09360.357467641(R) (T)
2225.09702225.1422-205395580(R) (D)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 17% (133/775AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 7/80 matches (8%).
Acc. #: Species: HUMAN Name: General transcription factor II-I repeat domain-containing protein 1 (GTF2I repeat domain containing protein 1) (Muscle TFII-I repeat domain-containing protein 1) (General transcription factor III) (Slow-muscle-fiber enhancer binding protein) (USE B1 binding protein) (MusTRD1/BEN) (Williams-Beuren syndrome chromosome region 11 protein)
Index: MW: 106058 Da pI: 6.4
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1383.67461383.6406256856951(K) (L)
1475.74071475.7494-5.91171290(R) (K)
1850.02461850.01763.87367530(K) (K)
2154.02892154.0674-1828470(K) (L)
2225.09702225.1892-413803991(K) (R)
2283.17002283.2211-225946130(K) (R)
2572.33992572.28302248711(K) (M)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 13% (126/959AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 6/80 matches (7%).
Acc. #: Species: HUMAN Name: Tyrosine-protein kinase-like 7 precursor (Colon carcinoma kinase-4) (CCK-4)
Index: MW: 118262 Da pI: 6.6
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1126.56451126.55677.0102610350(R) (L)
1126.56451126.5785-128058140(K) (A)
1756.90261756.9168-8.18468601(R) (L)
2179.09442179.051920105010700(K) (-)
2233.12242233.12080.714815010(R) (L)
2283.17002283.1702-0.109069251(K) (F)
2951.36902951.34906.81Met-ox 5045291(K)FTPPPQPQQCMGFDKEATVPCSATGR(E)
2951.36902951.4434-253734000(K) (R)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 14% (151/1070AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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