MS-Fit Search Results


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Parameters

Database searched: SwissProt.01.22.2003
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): 40-10
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 121920
Molecular weight search (1000 - 100000 Da) selects 115277 entries.
Full pI range: 121920 entries.
Species search ( HUMAN MOUSE ) selects 14893 entries.
Combined molecular weight, pI and species searches select 13163 entries.
Pre searches select 13163 entries.

Data Set 1 Results

MS-Fit search selects 458 entries (results displayed for top 5 matches).

Results Summary

MOWSE
Score		#/60(%)
Masses
Matched 		%
Cov		%
TIC		Mean
Err
ppm		Data
Tol
ppm		MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
15.136e+01018 (30)35.030.022.429.8 76716/6.4HUMANGMP synthase [glutamine-hydrolyzing] (Glutamine amidotransferase) (GMP synthetase)
23.654e+0059 (15)19.015.021.331.1 66018/8.2HUMANKeratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
36.891e+0049 (15)20.015.010.145.8 80509/5.5HUMANAdseverin (Scinderin)
45.961e+00410 (16)20.016.76.9157.0 73244/6.7HUMANDEAD-box protein 3 (Helicase-like protein 2) (HLP2) (DEAD-box, X isoform)
51.811e+0047 (11)22.011.7-0.82881.4 43998/5.2HUMANInositol polyphosphate 1-phosphatase (IPPase) (IPP)
94391x.xx..x.x..xx.x..x.........x...........x..xx..xx.x..x..x....
48753.x.......x...x.x....x..x....x.x.........x...................
11560...x.........x..........x.x.............x.xx.x.........x....
3614.....x.x.......x.x...........x.....x.x......x..x........x...
66456..x..............x....x...x...x..............x............x.


Detailed Results

1. 18/60 matches (30%).
Acc. #: Species: HUMAN Name: GMP synthase [glutamine-hydrolyzing] (Glutamine amidotransferase) (GMP synthetase)
Index: MW: 76716 Da pI: 6.4
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
864.4911864.48655.41Met-ox 6656711(K)MVTEIKK(I)
992.6053992.60054.84784850(K) (V)
1036.55561036.5679-124684770(K) (K)
1118.66551118.66143.65605690(R) (E)
1161.66861161.6533134364460(R) (V)
1235.64451235.6133253903990(K) (K)
1241.69341241.6741154254350(R) (H)
1296.67101296.633729pyroGlu 5906000(R)QADFEAHNILR(E)
1313.68941313.6602225906000(R) (E)
1590.81881590.7804245365480(K) (L)
1986.98161986.9231291251410(R) (G)
2071.09642071.0395271Met-ox 2592760(R)ALNQEQVIAVHIDNGFMR(K)
2077.06192076.9813392052220(K) (E)
2101.21622101.1691222392580(K) (A)
2145.21122145.1443315705890(K) (Q)
2237.16952237.07404319390(K) (V)
2364.37362364.2750421Met-ox 6086271(K)ISQMPVILTPLHFDRDPLQK(Q)
2421.26252421.1686392963170(K) (T)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 35% (247/693AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 9/60 matches (15%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
Index: MW: 66018 Da pI: 8.2
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
973.5241973.5318-8.03964030(K) (V)
1179.62161179.6010183773860(K) (H)
1277.73661277.7105204734830(K) (T)
1300.59031300.5302462582670(K) (N)
1383.71761383.6909191861970(K) (V)
1475.78751475.7494262122230(K) (T)
1475.78751475.78581.22002110(R) (W)
1688.01701687.97072766821(R) (G)
1716.90051716.8517284184320(K) (G)
1994.04561993.9772342242390(R) (R)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 19% (123/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 9/60 matches (15%).
Acc. #: Species: HUMAN Name: Adseverin (Scinderin)
Index: MW: 80509 Da pI: 5.5
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1036.55561036.5679-125655740(K) (C)
1277.73661277.7469-8.13593691(K) (Q)
1493.77861493.7276341501620(R) (G)
1588.79411588.7317393123240(K) (N)
1994.04561994.0783-161191381(K) (L)
2071.09642071.09232.02042221(K) (V)
2077.06192076.9878365805971(R) (D)
2097.04022096.9889245986150(K) (L)
2421.26252421.2818-8.03253451(K) (D)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 20% (143/715AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 10/60 matches (16%).
Acc. #: Species: HUMAN Name: DEAD-box protein 3 (Helicase-like protein 2) (HLP2) (DEAD-box, X isoform)
Index: MW: 73244 Da pI: 6.7
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1090.54231090.54210.224194270(K) (R)
1157.60311157.5876133643730(R) (G)
1300.59031300.6398-384794881(R) (S)
1313.68941313.68870.473633731(R) (G)
1707.82341707.8964-433123261(R) (L)
1838.99161838.9513225325481(R) (N)
1940.99291940.97927.13363511(K) (M)
2083.09792083.0249355125280(K) (I)
2145.21122145.1351352933111(R) (D)
2662.52472662.4278362312551(K) (E)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 20% (139/662AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 7/60 matches (11%).
Acc. #: Species: HUMAN Name: Inositol polyphosphate 1-phosphatase (IPPase) (IPP)
Index: MW: 43998 Da pI: 5.2
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
992.6053992.5641413703781(K) (K)
1313.68941313.6425363153250(K) (A)
1434.77481434.70514963741(K) (N)
1588.79411588.8586-4124360(R) (K)
1716.90051716.9536-3124371(R) (E)
2097.04022097.1232-403813990(R) (-)
2717.20862717.2638-202172390(K) (R)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 22% (88/399AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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