MS-Fit Search Results

MS-Fit Search Results

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Parameters

Database searched: SwissProt.10.25.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): 40-17
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 116819
Molecular weight search (1000 - 100000 Da) selects 110413 entries.
Full pI range: 116819 entries.
Species search ( HUMAN MOUSE ) selects 14257 entries.
Combined molecular weight, pI and species searches select 12612 entries.
Pre searches select 12612 entries.

Data Set 1 Results

MS-Fit search selects 21 entries (results displayed for top 5 matches).

Results Summary

	MOWSE Score	#/41(%) Masses Matched	% Cov	% TIC	Mean Err ppm	Data Tol ppm	MS-Digest Index #	Protein MW (Da)/pI	Accession #	Species	Protein Name
1	2.252e+007	17 (41)	31.0	41.5	1.14	10.9		67878/7.6		HUMAN	Transketolase (TK)
2	1.339e+004	9 (21)	14.0	22.0	2.21	7.08		67631/7.2		MOUSE	TRANSKETOLASE (TK) (P68)
3	381	4 (9)	7.0	9.8	7.38	7.64		95432/8.3		HUMAN	Oxysterol binding protein-related protein 7 (OSBP-related protein 7) (ORP-7)
4	324	4 (9)	12.0	9.8	0.768	21.0		43002/8.9		MOUSE	Chitinase-3 like protein 1 precursor (Cartilage glycoprotein-39) (GP-39) (BRP39 protein)
5	149	4 (9)	3.0	9.8	7.02	38.4		74744/6.4		HUMAN	High-affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8B

3393	x	.	x	.	x	x	x	.	.	.	x	.	x	.	.	.	x	.	.	.	.	x	x	.	x	x	.	.	.	x	.	.	x	x	.	.	x	x	.	.	.
87737	x	.	x	.	x	.	x	.	.	.	x	.	.	.	.	.	.	.	.	.	.	x	.	x	.	x	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.
64999	.	.	.	.	.	.	.	.	.	.	.	.	.	x	x	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.
20944	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	x	x	.	.	.	.	.	.	.	.	.	.
49732	.	.	x	.	.	.	.	.	.	.	.	.	x	x	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.

Detailed Results

1. 17/41 matches (41%).
Acc. #: Species: HUMAN Name: Transketolase (TK)
Index: MW: 67878 Da pI: 7.6

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
915.5686	915.5627	6.3		543	550	1	(R) (A)
1200.6361	1200.6265	8.0		344	353	1	(K) (E)
1326.6899	1326.6919	-1.5		65	75	1	(R) (G)
1368.7889	1368.7851	2.8		175	186	0	(K) (L)
1413.8106	1413.8106	0.033		531	542	0	(R) (K)
1541.9122	1541.9055	4.3		531	543	1	(R) (L)
1562.7906	1562.8113	-13		604	617	1	(K) (G)
1578.8055	1578.8062	-0.47	1Met-ox	604	617	1	(K)MFGIDRDAIAQAVR(G)
1665.8162	1665.8099	3.7		382	395	0	(R) (A)
1884.9229	1884.9231	-0.11		439	456	0	(R) (A)
1978.0630	1978.0398	12		247	264	1	(R) (N)
2020.0744	2020.0715	1.5		284	302	0	(K) (M)
2039.0102	2039.0119	-0.85	1Met-ox	265	281	0	(K)NMAEQIIQEIYSQIQSK(K)
2085.0146	2085.0075	3.4	1Met-ox	187	204	0	(R)LGQSDPAPLQHQMDIYQK(R)
2148.1636	2148.1665	-1.3		283	302	1	(K) (M)
2241.0997	2241.1086	-4.0	1Met-ox	187	205	1	(R)LGQSDPAPLQHQMDIYQKR(C)
2508.2092	2508.2119	-1.1		472	493	0	(R) (V)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 31% (195/623AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 9/41 matches (21%).
Acc. #: Species: MOUSE Name: TRANSKETOLASE (TK) (P68)
Index: MW: 67631 Da pI: 7.2

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
915.5686 915.5627 6.3 543 550 1 (R) (A)
1200.6361 1200.6265 8.0 344 353 1 (K) (E)
1326.6899 1326.6919 -1.5 65 75 1 (R) (G)
1413.8106 1413.8106 0.033 531 542 0 (R) (K)
1541.9122 1541.9055 4.3 531 543 1 (R) (L)
1665.8162 1665.8099 3.7 382 395 0 (R) (A)
1976.0581 1976.0605 -1.2 247 264 1 (R) (N)
2020.0744 2020.0715 1.5 284 302 0 (K) (M)
2148.1636 2148.1665 -1.3 283 302 1 (K) (M)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 14% (93/623AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 4/41 matches (9%).
Acc. #: Species: HUMAN Name: Oxysterol binding protein-related protein 7 (OSBP-related protein 7) (ORP-7)
Index: MW: 95432 Da pI: 8.3

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1563.7817 1563.7655 10 767 780 0 (R) (R)
1567.8177 1567.8015 10 792 804 1 (R) (F)
1577.8178 1577.8076 6.4 126 138 0 (K) (A)
2523.1820 2523.1761 2.4 551 571 0 (R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 7% (61/842AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 4/41 matches (9%).
Acc. #: Species: MOUSE Name: Chitinase-3 like protein 1 precursor (Cartilage glycoprotein-39) (GP-39) (BRP39 protein)
Index: MW: 43002 Da pI: 8.9

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1560.7932 1560.7923 0.60 pyroGlu 215 228 0 (R)QITGHHSPLFQGQK(D)
1577.8178 1577.8188 -0.69 215 228 0 (R) (D)
2039.0102 2038.9809 14 232 247 1 (R) (L)
2042.0271 2042.0500 -11 130 146 1 (R) (D)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 12% (47/381AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 4/41 matches (9%).
Acc. #: Species: HUMAN Name: High-affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8B
Index: MW: 74744 Da pI: 6.4

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1200.6361 1200.6085 23 201 211 1 (R) (Y)
1562.7906 1562.7810 6.2 pyroGlu 1Met-ox 492 505 0 (R)QAIIDMVLATEMTK(H)
1563.7817 1563.8126 -20 492 505 0 (R) (H)
1578.8055 1578.7759 19 pyroGlu 2Met-ox 492 505 0 (R)QAIIDMVLATEMTK(H)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 3% (25/659AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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