MS-Fit Search Results


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Parameters

Database searched: SwissProt.01.22.2003
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): 40-22
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 121920
Molecular weight search (1000 - 100000 Da) selects 115277 entries.
Full pI range: 121920 entries.
Species search ( HUMAN MOUSE ) selects 14893 entries.
Combined molecular weight, pI and species searches select 13163 entries.
Pre searches select 13163 entries.

Data Set 1 Results

MS-Fit search selects 761 entries (results displayed for top 10 matches).

Results Summary

MOWSE
Score		#/90(%)
Masses
Matched 		%
Cov		%
TIC		Mean
Err
ppm		Data
Tol
ppm		MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
17.840e+00713 (14)27.014.4-49.448.6 66018/8.2HUMANKeratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
21.098e+00610 (11)32.011.1-30.652.6 61988/5.1HUMANKeratin, type I cytoskeletal 9 (Cytokeratin 9) (K9) (CK 9)
36.212e+0047 (7)14.07.8-5.1360.1 76765/6.6HUMANProtein kinase C, alpha type (PKC-alpha) (PKC-A)
42.336e+0047 (7)21.07.8-48.830.8 59519/5.1HUMANKeratin, type I cytoskeletal 10 (Cytokeratin 10) (K10) (CK 10)
51.078e+0045 (5)23.05.6-53.171.2 39806/9.0HUMANHigh affinity interleukin-8 receptor A (IL-8R A) (IL-8 receptor type 1) (CXCR-1) (CDw128a)
61.004e+0047 (7)10.07.8-46.268.1 86480/6.4HUMANProtein transport protein Sec23B (SEC23-related protein B)
759887 (7)18.07.8-56.447.3 72912/7.0HUMANAlkyldihydroxyacetonephosphate synthase, peroxisomal precursor (Alkyl-DHAP synthase) (Alkylglycerone-phosphate synthase)
856917 (7)19.07.8-33.242.6 78440/6.9HUMANPR-domain zinc finger protein 17
938676 (6)22.06.7-36.8110 39135/8.9HUMANBlue-sensitive opsin (Blue cone photoreceptor pigment)
1036138 (8)13.08.9-37.387.6 96306/5.6MOUSETuftelin-interacting protein 11 (Tuftelin-interacting protein 39)
48753.............x.......xx......x.x.......x.....x.x.xx..........x.......x........x...........
104932.....................x..x...........................x..x.x................x....x..x....x.x
4539........................x.x.............................x.....x.x...........xx............
116573......x.........................x........x......x......................x....x.........x...
9768.....................x..............x..................x..........x....................x..
99009.....x...........x..x...........................................x....x...xx...............
42776.......x.........................x................x....x..........x...x...............x...
76092........................x.......x.......................x.x.....x.....x..................x
99802.................................x.x..x............................x.......x.....x........
33531.....................x....x...........x..x.x.............................xx.....x.........


Detailed Results

1. 13/90 matches (14%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
Index: MW: 66018 Da pI: 8.2
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1157.48051157.5149-301Met-ox 4644720(R)DYQELMNTK(L)
1277.60871277.6337-202472571(K) (N)
1277.60871277.7105-804734830(K) (T)
1300.44841300.5302-632582670(K) (N)
1357.60731357.6963-664444550(K) (E)
1383.58971383.6909-731861970(K) (V)
1475.64831475.7494-692122230(K) (T)
1657.69711657.7934-5813290(R) (R)
1699.71101699.8251-67pyroGlu 4184320(K)QISNLQQSISDAEQR(G)
1716.75441716.8517-574184320(K) (G)
1751.83951751.82727.06036241(R) (S)
1993.88191993.9772-482242390(R) (R)
2149.99312150.0783-402242401(R) (R)
2383.88072383.9524-305195490(R) (G)

Click link below to search for cysteine linked fragments.

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The matched peptides cover 27% (179/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 10/90 matches (11%).
Acc. #: Species: HUMAN Name: Keratin, type I cytoskeletal 9 (Cytokeratin 9) (K9) (CK 9)
Index: MW: 61988 Da pI: 5.1
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1277.60871277.58751729401(R) (G)
1307.57801307.6782-772402491(R) (Q)
1791.66271791.7283-374905120(R) (G)
1837.86071837.9660-573743890(R) (K)
1851.83811851.9275-483213351(K) (N)
2299.09192299.1202-122712891(K) (N)
2510.07902510.1323-214494710(K) (I)
2705.07972705.1617-3063940(R) (G)
2902.32432902.4111-301992231(K) (T)
3264.48023264.5144-103393670(K) (E)

Click link below to search for cysteine linked fragments.

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The matched peptides cover 32% (204/622AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 7/90 matches (7%).
Acc. #: Species: HUMAN Name: Protein kinase C, alpha type (PKC-alpha) (PKC-A)
Index: MW: 76765 Da pI: 6.6
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1307.57801307.5183461321410(K) (Q)
1323.54651323.5132251Met-ox 1321410(K)CDTCDMNVHK(Q)
1838.83691838.83182.877911(K) (G)
1838.83691838.8504-7.463760(K) (R)
1998.89481998.9087-7.0pyroGlu 1421580(K)QCVINVPSLCGMDHTEK(R)
2015.86222015.9353-361421580(K) (R)
2367.15312367.1985-192172361(R) (D)
2368.98792369.0944-451221(-) (K)

Click link below to search for cysteine linked fragments.

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The matched peptides cover 14% (98/672AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 7/90 matches (7%).
Acc. #: Species: HUMAN Name: Keratin, type I cytoskeletal 10 (Cytokeratin 10) (K10) (CK 10)
Index: MW: 59519 Da pI: 5.1
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1060.45191060.4733-201Met-ox 2852921(K)KNHEEEMK(D)
1060.45191060.5097-541Met-ox 191(-)MSVRYSSSK(H)
1390.60791390.6814-533873990(K) (Y)
1493.62941493.7348-713233341(R) (D)
1707.68361707.7727-5241590(K) (G)
2224.01132224.1423-592672851(K) (N)
2367.15312367.2632-472082280(K) (L)
2872.29442872.3933-342963220(R) (S)

Click link below to search for cysteine linked fragments.

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The matched peptides cover 21% (127/593AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 5/90 matches (5%).
Acc. #: Species: HUMAN Name: High affinity interleukin-8 receptor A (IL-8R A) (IL-8 receptor type 1) (CXCR-1) (CDw128a)
Index: MW: 39806 Da pI: 9.0
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1277.60871277.6160-5.72702790(R) (R)
1455.63071455.7459-791001110(K) (V)
1837.86071837.9044-243343501(R) (-)
2082.92862083.0898-771181350(K) (Y)
2902.32432902.5556-802042270(R) (T)

Click link below to search for cysteine linked fragments.

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The matched peptides cover 23% (81/350AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

6. 7/90 matches (7%).
Acc. #: Species: HUMAN Name: Protein transport protein Sec23B (SEC23-related protein B)
Index: MW: 86480 Da pI: 6.4
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1033.41781033.4889-693323390(K) (T)
1201.48501201.5701-715365460(R) (W)
1265.51311265.6067-7419280(R) (L)
2015.86222016.0164-764794951(R) (I)
2149.99312149.98314.75896060(R) (H)
2283.06932283.1266-251721901(R) (G)
2299.09192299.1215-131Met-ox 1721901(R)MVQVHELSCEGISKSYVFR(G)

Click link below to search for cysteine linked fragments.

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The matched peptides cover 10% (83/767AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

7. 7/90 matches (7%).
Acc. #: Species: HUMAN Name: Alkyldihydroxyacetonephosphate synthase, peroxisomal precursor (Alkyl-DHAP synthase) (Alkylglycerone-phosphate synthase)
Index: MW: 72912 Da pI: 7.0
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1065.40341065.4886-8051590(R) (A)
1405.58121405.6932-801Met-ox 4134241(R)CAPASIRLMDNK(Q)
1751.83951751.8717-186416551(K) (N)
1837.86071837.9522-506256401(K) (S)
2082.92862083.0671-6663850(R) (K)
2211.03392211.1621-5863861(R) (R)
2211.03392211.1960-733243421(K) (G)
2872.29442872.3683-262282530(K) (T)

Click link below to search for cysteine linked fragments.

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The matched peptides cover 18% (121/658AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

8. 7/90 matches (7%).
Acc. #: Species: HUMAN Name: PR-domain zinc finger protein 17
Index: MW: 78440 Da pI: 6.9
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1307.57801307.6166-303093190(K) (A)
1390.60791390.6776-501120(-) (K)
1838.83691838.8682-175265400(R) (R)
1940.84861940.9145-341Met-ox 6096241(R)CPTCGMGYTLPQSLRR(H)
2015.86222015.9861-6123410(R) (D)
2211.03392211.01886.84264440(K) (A)
2211.03392211.1068-334895061(R) (Q)
3264.48023264.6363-4870981(K) (E)

Click link below to search for cysteine linked fragments.

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The matched peptides cover 19% (139/720AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

9. 6/90 matches (6%).
Acc. #: Species: HUMAN Name: Blue-sensitive opsin (Blue cone photoreceptor pigment)
Index: MW: 39135 Da pI: 8.9
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1405.58121405.6811-713353480(K) (-)
1450.58481450.6928-743131(R) (N)
1460.62841460.7345-732292420(K) (A)
2094.94662094.8452482Met-ox 3173341(K)MVCGKAMTDESDTCSSQK(T)
2342.91792343.0790-691751940(R) (Y)
2662.29012662.2434181751961(R) (S)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

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The matched peptides cover 22% (79/348AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

10. 8/90 matches (8%).
Acc. #: Species: MOUSE Name: Tuftelin-interacting protein 11 (Tuftelin-interacting protein 39)
Index: MW: 96306 Da pI: 5.6
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1277.60871277.5261651Met-ox 1391480(K)SFMDFGSWER(H)
1277.60871277.7040-751Met-ox 1571671(K)LLQKMGYVPGR(G)
1323.54651323.5817-2759700(R) (R)
1460.62841460.6480-131Met-ox 7427521(R)KDFQYEAMQER(R)
1493.62941493.7438-774814910(R) (N)
1507.63531507.7327-657157290(R) (E)
2283.06932283.2059-607767951(K) (R)
2299.09192299.2008-471Met-ox 7767951(K)DLIETKAEEHNIVFMPVIGK(R)
2565.07952565.1759-3823421(R) (H)

Click link below to search for cysteine linked fragments.

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The matched peptides cover 13% (110/838AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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