MS-Fit Search Results

Parameters

Database searched: SwissProt.01.22.2003
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): 40-24
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 121920
Molecular weight search (1000 - 100000 Da) selects 115277 entries.
Full pI range: 121920 entries.
Species search ( HUMAN MOUSE ) selects 14893 entries.
Combined molecular weight, pI and species searches select 13163 entries.
Pre searches select 13163 entries.

Data Set 1 Results

MS-Fit search selects 364 entries (results displayed for top 5 matches).

Results Summary

	MOWSE Score	#/60(%) Masses Matched	% Cov	% TIC	Mean Err ppm	Data Tol ppm	Protein MW (Da)/pI	Species	Protein Name
1	1.615e+007	19 (31)	46.0	31.7	-2.43	28.2	58519/9.3	HUMAN	Polyadenylate-binding protein 2 (Poly(A) binding protein 2) (PABP 2)
2	8.967e+006	18 (30)	35.0	30.0	-2.14	28.8	70643/9.5	MOUSE	Polyadenylate-binding protein 1 (Poly(A)-binding protein 1) (PABP 1) (PABP1)
3	8.964e+006	19 (31)	37.0	31.7	-2.43	28.2	70671/9.5	HUMAN	Polyadenylate-binding protein 1 (Poly(A)-binding protein 1) (PABP 1) (PABP1)
4	1.383e+004	9 (15)	11.0	15.0	-20.8	42.6	95203/8.9	MOUSE	Glutamate receptor, ionotropic kainate 1 precursor (Glutamate receptor 5) (GluR-5) (GluR5)
5	7967	8 (13)	18.0	13.3	-15.4	50.7	72912/7.0	HUMAN	Alkyldihydroxyacetonephosphate synthase, peroxisomal precursor (Alkyl-DHAP synthase) (Alkylglycerone-phosphate synthase)

56101	.	x	x	x	x	.	x	x	.	.	.	x	x	.	x	.	x	x	x	.	.	x	.	x	x	.	.	x	.	.	.	.	.	.	.	x	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.
102304	.	x	x	x	x	.	x	x	.	.	.	x	x	.	x	.	x	x	x	.	.	.	.	x	x	.	.	x	.	.	.	.	.	.	.	x	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.
102345	.	x	x	x	x	.	x	x	.	.	.	x	x	.	x	.	x	x	x	.	.	x	.	x	x	.	.	x	.	.	.	.	.	.	.	x	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.
5069	.	x	.	.	x	.	.	.	.	.	.	.	.	.	.	x	.	.	.	x	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	x	.	.	.	.	x	.	.	.	.	x	.	x	.	.	.	.	.	.	.	.	.
42776	.	.	.	.	.	.	.	x	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	x	.	.	.	x	.	.	x	.	.	.	.	.	.	.	x	.	x

Detailed Results

1. 19/60 matches (31%).
Acc. #: Species: HUMAN Name: Polyadenylate-binding protein 2 (Poly(A) binding protein 2) (PABP 2)
Index: MW: 58519 Da pI: 9.3

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
1045.5211	1045.5107	9.9		207	215	0	(K) (H)
1083.5738	1083.5839	-9.3		266	274	0	(R) (N)
1157.6732	1157.6683	4.2		6	16	0	(K) (V)
1161.5157	1161.5024	11		275	284	0	(K) (L)
1212.6624	1212.6628	-0.39		162	171	1	(R) (N)
1282.6191	1282.6353	-13	1Met-ox	43	53	0	(R)ALDTMNFDVIK(G)
1388.6527	1388.6381	11	pyroGlu 1Met-ox	350	360	0	(R)QAHLTNQYMQR(M)
1388.6527	1388.7109	-42		59	69	1	(R) (K)
1405.6631	1405.6647	-1.1	1Met-ox	350	360	0	(R)QAHLTNQYMQR(M)
1430.6684	1430.6876	-13		275	286	1	(K) (K)
1484.6717	1484.6327	26	1Met-ox	172	183	1	(K)NFGEDMDDERLK(D)
1520.7604	1520.7565	2.5	1Met-ox	129	141	1	(R)AIEKMNGMLLNDR(K)
1542.9388	1542.9372	1.1		332	345	0	(R) (K)
1652.9068	1652.9198	-7.9	1Met-ox	452	466	0	(R)LFPLIQAMHPTLAGK(I)
1676.7599	1676.7630	-1.9		309	323	0	(K) (A)
1693.8885	1693.9237	-21		491	506	1	(R) (E)
1740.8025	1740.7982	2.5		114	128	0	(K) (A)
1820.9007	1820.8893	6.3		89	104	0	(K) (V)
1928.9056	1928.9143	-4.5		26	42	0	(R) (A)
2772.3533	2772.3800	-9.6	2Met-ox	467	490	0	(K)ITGMLLEIDNSELLHMLESPESLR(S)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 46% (241/522AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 18/60 matches (30%).
Acc. #: Species: MOUSE Name: Polyadenylate-binding protein 1 (Poly(A)-binding protein 1) (PABP 1) (PABP1)
Index: MW: 70643 Da pI: 9.5

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1045.5211 1045.5107 9.9 232 240 0 (K) (H)
1083.5738 1083.5839 -9.3 291 299 0 (R) (N)
1157.6732 1157.6683 4.2 31 41 0 (K) (V)
1161.5157 1161.5024 11 300 309 0 (K) (L)
1212.6624 1212.6628 -0.39 187 196 1 (R) (N)
1282.6191 1282.6353 -13 1Met-ox 68 78 0 (R)ALDTMNFDVIK(G)
1388.6527 1388.6381 11 pyroGlu 1Met-ox 375 385 0 (R)QAHLTNQYMQR(M)
1388.6527 1388.7109 -42 84 94 1 (R) (K)
1405.6631 1405.6647 -1.1 1Met-ox 375 385 0 (R)QAHLTNQYMQR(M)
1430.6684 1430.6876 -13 300 311 1 (K) (K)
1484.6717 1484.6327 26 1Met-ox 197 208 1 (K)NFGEDMDDERLK(E)
1520.7604 1520.7565 2.5 1Met-ox 154 166 1 (R)AIEKMNGMLLNDR(K)
1542.9388 1542.9372 1.1 357 370 0 (R) (K)
1676.7599 1676.7630 -1.9 334 348 0 (K) (A)
1693.8885 1693.9237 -21 605 620 1 (R) (E)
1740.8025 1740.7982 2.5 139 153 0 (K) (A)
1820.9007 1820.8893 6.3 114 129 0 (K) (V)
1928.9056 1928.9143 -4.5 51 67 0 (R) (A)
2772.3533 2772.3800 -9.6 2Met-ox 581 604 0 (K)ITGMLLEIDNSELLHMLESPESLR(S)

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
1045.5211	1045.5107	9.9		232	240	0	(K) (H)
1083.5738	1083.5839	-9.3		291	299	0	(R) (N)
1157.6732	1157.6683	4.2		31	41	0	(K) (V)
1161.5157	1161.5024	11		300	309	0	(K) (L)
1212.6624	1212.6628	-0.39		187	196	1	(R) (N)
1282.6191	1282.6353	-13	1Met-ox	68	78	0	(R)ALDTMNFDVIK(G)
1388.6527	1388.6381	11	pyroGlu 1Met-ox	375	385	0	(R)QAHLTNQYMQR(M)
1388.6527	1388.7109	-42		84	94	1	(R) (K)
1405.6631	1405.6647	-1.1	1Met-ox	375	385	0	(R)QAHLTNQYMQR(M)
1430.6684	1430.6876	-13		300	311	1	(K) (K)
1484.6717	1484.6327	26	1Met-ox	197	208	1	(K)NFGEDMDDERLK(E)
1520.7604	1520.7565	2.5	1Met-ox	154	166	1	(R)AIEKMNGMLLNDR(K)
1542.9388	1542.9372	1.1		357	370	0	(R) (K)
1676.7599	1676.7630	-1.9		334	348	0	(K) (A)
1693.8885	1693.9237	-21		605	620	1	(R) (E)
1740.8025	1740.7982	2.5		139	153	0	(K) (A)
1820.9007	1820.8893	6.3		114	129	0	(K) (V)
1928.9056	1928.9143	-4.5		51	67	0	(R) (A)
2772.3533	2772.3800	-9.6	2Met-ox	581	604	0	(K)ITGMLLEIDNSELLHMLESPESLR(S)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 35% (226/636AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 19/60 matches (31%).
Acc. #: Species: HUMAN Name: Polyadenylate-binding protein 1 (Poly(A)-binding protein 1) (PABP 1) (PABP1)
Index: MW: 70671 Da pI: 9.5

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1045.5211 1045.5107 9.9 232 240 0 (K) (H)
1083.5738 1083.5839 -9.3 291 299 0 (R) (N)
1157.6732 1157.6683 4.2 31 41 0 (K) (V)
1161.5157 1161.5024 11 300 309 0 (K) (L)
1212.6624 1212.6628 -0.39 187 196 1 (R) (N)
1282.6191 1282.6353 -13 1Met-ox 68 78 0 (R)ALDTMNFDVIK(G)
1388.6527 1388.6381 11 pyroGlu 1Met-ox 375 385 0 (R)QAHLTNQYMQR(M)
1388.6527 1388.7109 -42 84 94 1 (R) (K)
1405.6631 1405.6647 -1.1 1Met-ox 375 385 0 (R)QAHLTNQYMQR(M)
1430.6684 1430.6876 -13 300 311 1 (K) (K)
1484.6717 1484.6327 26 1Met-ox 197 208 1 (K)NFGEDMDDERLK(D)
1520.7604 1520.7565 2.5 1Met-ox 154 166 1 (R)AIEKMNGMLLNDR(K)
1542.9388 1542.9372 1.1 357 370 0 (R) (K)
1652.9068 1652.9198 -7.9 1Met-ox 566 580 0 (R)LFPLIQAMHPTLAGK(I)
1676.7599 1676.7630 -1.9 334 348 0 (K) (A)
1693.8885 1693.9237 -21 605 620 1 (R) (E)
1740.8025 1740.7982 2.5 139 153 0 (K) (A)
1820.9007 1820.8893 6.3 114 129 0 (K) (V)
1928.9056 1928.9143 -4.5 51 67 0 (R) (A)
2772.3533 2772.3800 -9.6 2Met-ox 581 604 0 (K)ITGMLLEIDNSELLHMLESPESLR(S)

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
1045.5211	1045.5107	9.9		232	240	0	(K) (H)
1083.5738	1083.5839	-9.3		291	299	0	(R) (N)
1157.6732	1157.6683	4.2		31	41	0	(K) (V)
1161.5157	1161.5024	11		300	309	0	(K) (L)
1212.6624	1212.6628	-0.39		187	196	1	(R) (N)
1282.6191	1282.6353	-13	1Met-ox	68	78	0	(R)ALDTMNFDVIK(G)
1388.6527	1388.6381	11	pyroGlu 1Met-ox	375	385	0	(R)QAHLTNQYMQR(M)
1388.6527	1388.7109	-42		84	94	1	(R) (K)
1405.6631	1405.6647	-1.1	1Met-ox	375	385	0	(R)QAHLTNQYMQR(M)
1430.6684	1430.6876	-13		300	311	1	(K) (K)
1484.6717	1484.6327	26	1Met-ox	197	208	1	(K)NFGEDMDDERLK(D)
1520.7604	1520.7565	2.5	1Met-ox	154	166	1	(R)AIEKMNGMLLNDR(K)
1542.9388	1542.9372	1.1		357	370	0	(R) (K)
1652.9068	1652.9198	-7.9	1Met-ox	566	580	0	(R)LFPLIQAMHPTLAGK(I)
1676.7599	1676.7630	-1.9		334	348	0	(K) (A)
1693.8885	1693.9237	-21		605	620	1	(R) (E)
1740.8025	1740.7982	2.5		139	153	0	(K) (A)
1820.9007	1820.8893	6.3		114	129	0	(K) (V)
1928.9056	1928.9143	-4.5		51	67	0	(R) (A)
2772.3533	2772.3800	-9.6	2Met-ox	581	604	0	(K)ITGMLLEIDNSELLHMLESPESLR(S)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 37% (241/636AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 9/60 matches (15%).
Acc. #: Species: MOUSE Name: Glutamate receptor, ionotropic kainate 1 precursor (Glutamate receptor 5) (GluR-5) (GluR5)
Index: MW: 95203 Da pI: 8.9

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1045.5211 1045.5278 -6.4 751 759 1 (K) (S)
1161.5157 1161.5614 -39 687 696 1 (R) (S)
1475.7527 1475.7203 22 420 432 1 (R) (T)
1552.7670 1552.8223 -36 172 185 0 (K) (L)
1748.8068 1748.8377 -18 664 679 1 (R) (I)
1838.8978 1838.9396 -23 786 801 0 (K) (N)
1940.9492 1941.0407 -47 433 448 0 (R) (K)
2209.0643 2209.1361 -32 786 804 1 (K) (K)
2225.1142 2225.1310 -7.6 1Met-ox 786 804 1 (K)CLSFNAIMEELGISLKNQK(K)

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
1045.5211	1045.5278	-6.4		751	759	1	(K) (S)
1161.5157	1161.5614	-39		687	696	1	(R) (S)
1475.7527	1475.7203	22		420	432	1	(R) (T)
1552.7670	1552.8223	-36		172	185	0	(K) (L)
1748.8068	1748.8377	-18		664	679	1	(R) (I)
1838.8978	1838.9396	-23		786	801	0	(K) (N)
1940.9492	1941.0407	-47		433	448	0	(R) (K)
2209.0643	2209.1361	-32		786	804	1	(K) (K)
2225.1142	2225.1310	-7.6	1Met-ox	786	804	1	(K)CLSFNAIMEELGISLKNQK(K)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 11% (97/836AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 8/60 matches (13%).
Acc. #: Species: HUMAN Name: Alkyldihydroxyacetonephosphate synthase, peroxisomal precursor (Alkyl-DHAP synthase) (Alkylglycerone-phosphate synthase)
Index: MW: 72912 Da pI: 7.0

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1282.6191 1282.6353 -13 629 640 0 (K) (S)
1405.6631 1405.6932 -21 1Met-ox 413 424 1 (R)CAPASIRLMDNK(Q)
1820.9007 1820.9257 -14 pyroGlu 625 640 1 (K)QWLKESISDVGFGMLK(S)
1928.9056 1928.9329 -14 390 406 0 (K) (E)
2082.9990 2083.0671 -33 63 85 0 (R) (K)
2211.1076 2211.1621 -25 63 86 1 (R) (R)
2211.1076 2211.1960 -40 324 342 1 (K) (G)
2772.3533 2772.2211 48 293 317 0 (K) (A)
3039.4690 3039.5515 -27 381 406 1 (K) (E)

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
1282.6191	1282.6353	-13		629	640	0	(K) (S)
1405.6631	1405.6932	-21	1Met-ox	413	424	1	(R)CAPASIRLMDNK(Q)
1820.9007	1820.9257	-14	pyroGlu	625	640	1	(K)QWLKESISDVGFGMLK(S)
1928.9056	1928.9329	-14		390	406	0	(K) (E)
2082.9990	2083.0671	-33		63	85	0	(R) (K)
2211.1076	2211.1621	-25		63	86	1	(R) (R)
2211.1076	2211.1960	-40		324	342	1	(K) (G)
2772.3533	2772.2211	48		293	317	0	(K) (A)
3039.4690	3039.5515	-27		381	406	1	(K) (E)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 18% (122/658AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):