MS-Fit Search Results

MS-Fit Search Results

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Parameters

Database searched: SwissProt.01.22.2003
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): 40-26
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 121920
Molecular weight search (10000 - 100000 Da) selects 104019 entries.
Full pI range: 121920 entries.
Species search ( HUMAN MOUSE ) selects 14893 entries.
Combined molecular weight, pI and species searches select 12734 entries.
Pre searches select 12734 entries.

Data Set 1 Results

MS-Fit search selects 1456 entries (results displayed for top 5 matches).

Results Summary

	MOWSE Score	#/78(%) Masses Matched	% Cov	% TIC	Mean Err ppm	Data Tol ppm	MS-Digest Index #	Protein MW (Da)/pI	Accession #	Species	Protein Name
1	2.787e+006	11 (14)	23.0	14.1	-60.0	44.3		66194/6.2		HUMAN	WD-repeat protein 1 (Actin interacting protein 1) (AIP1) (NORI-1)
2	4.561e+005	25 (32)	49.0	32.1	-62.4	56.9		62640/6.4		HUMAN	Stress-induced-phosphoprotein 1 (STI1) (Hsp70/Hsp90-organizing protein) (Transformation-sensitive protein IEF SSP 3521)
3	9.036e+004	11 (14)	23.0	14.1	-65.4	40.5		64616/6.3		HUMAN	Bifunctional purine biosynthesis protein PURH [Includes: Phosphoribosylaminoimidazolecarboxamide formyltransferase (AICAR transformylase); IMP cyclohydrolase (Inosinicase) (IMP synthetase) (ATIC)]
4	9251	9 (11)	13.0	11.5	-28.1	90.7		96042/4.8		MOUSE	Neurofilament triplet M protein (160 kDa neurofilament protein) (Neurofilament medium polypeptide) (NF-M)
5	7063	6 (7)	46.0	7.7	-37.6	79.4		20791/5.4		MOUSE	Apoptosis regulator Bcl-W (BCL2-like 2 protein)

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Detailed Results

1. 11/78 matches (14%).
Acc. #: Species: HUMAN Name: WD-repeat protein 1 (Actin interacting protein 1) (AIP1) (NORI-1)
Index: MW: 66194 Da pI: 6.2

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
899.3796	899.4661	-96	1Met-ox	390	396	0	(R)YTSLMLR(D)
1102.4376	1102.5209	-76		96	104	0	(K) (I)
1145.5465	1145.6319	-74		8	17	0	(K) (G)
1273.6146	1273.7268	-88		7	17	1	(K) (G)
1618.6839	1618.7753	-56		66	81	0	(K) (L)
1719.7328	1719.8230	-52		204	219	0	(R) (T)
1887.9130	1887.9605	-25		66	83	1	(K) (I)
2057.8617	2057.9279	-32		162	180	0	(R) (F)
2207.9455	2208.0726	-58		127	147	0	(K) (V)
2293.9410	2294.0763	-59		490	511	0	(K) (V)
2418.1380	2418.2417	-43		44	65	0	(R) (Y)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 23% (145/606AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 25/78 matches (32%).
Acc. #: Species: HUMAN Name: Stress-induced-phosphoprotein 1 (STI1) (Hsp70/Hsp90-organizing protein) (Transformation-sensitive protein IEF SSP 3521)
Index: MW: 62640 Da pI: 6.4

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
900.4381 900.5155 -86 154 160 0 (R) (N)
1004.4648 1004.5529 -88 79 87 0 (K) (F)
1007.4281 1007.4951 -66 318 325 0 (K) (S)
1022.3893 1022.4763 -85 278 284 1 (K) (A)
1022.3893 1022.4828 -91 454 462 0 (K) (E)
1063.3998 1063.4842 -79 1Met-ox 110 118 0 (K)EGLQNMEAR(L)
1065.4734 1065.5580 -79 145 153 0 (R) (E)
1116.5536 1116.6451 -82 1Met-ox 534 543 0 (K)LMDVGLIAIR(-)
1119.4448 1119.4385 5.6 2Met-ox 471 478 0 (R)CMMAQYNR(H)
1132.5635 1132.6479 -75 78 87 1 (R) (F)
1137.4772 1137.5904 -100 514 523 0 (K) (N)
1158.5143 1158.6006 -75 AcetN 1 10 1 (-)EQVNELKEK(G)
1158.5143 1158.6159 -88 64 73 0 (K) (G)
1214.5173 1214.6057 -73 306 315 1 (R) (Y)
1298.5652 1298.6533 -68 316 325 1 (K) (S)
1464.6435 1464.7348 -62 33 44 0 (K) (S)
1488.6924 1488.7950 -69 352 364 0 (R) (N)
1608.7629 1608.8386 -47 79 92 1 (K) (R)
1675.7048 1675.7613 -34 2Met-ox 124 136 0 (K)FMNPFNMPNLYQK(L)
1803.7703 1803.8562 -48 2Met-ox 123 136 1 (R)KFMNPFNMPNLYQK(L)
1870.8369 1870.9299 -50 94 109 1 (R) (E)
1887.9130 1888.0292 -62 514 530 1 (K) (I)
1929.8060 1929.9115 -55 1Met-ox 208 223 1 (K)ETKPEPMEEDLPENKK(Q)
1929.8060 1929.9115 -55 1Met-ox 207 222 1 (K)KETKPEPMEEDLPENK(K)
1937.7361 1937.8407 -54 3Met-ox 489 505 0 (R)AMADPEVQQIMSDPAMR(L)
2026.9094 2026.8421 33 2Met-ox 471 486 1 (R)CMMAQYNRHDSPEDVK(R)
2080.8710 2081.0225 -73 14 32 0 (K) (L)
2363.9992 2364.1070 -46 1Met-ox 253 272 0 (K)ELDPTNMTYITNQAAVYFEK(G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 49% (267/543AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 11/78 matches (14%).
Acc. #: Species: HUMAN Name: Bifunctional purine biosynthesis protein PURH [Includes: Phosphoribosylaminoimidazolecarboxamide formyltransferase (AICAR transformylase); IMP cyclohydrolase (Inosinicase) (IMP synthetase) (ATIC)]
Index: MW: 64616 Da pI: 6.3

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
890.4261 890.5100 -94 91 97 0 (R) (V)
900.4381 900.4791 -45 409 416 0 (K) (D)
1008.4210 1008.5056 -84 462 468 0 (K) (H)
1163.5582 1163.6424 -72 295 305 0 (K) (A)
1355.6703 1355.7912 -89 67 79 0 (K) (N)
1384.6250 1384.7297 -76 438 451 0 (K) (I)
1464.6435 1464.7334 -61 554 569 0 (R) (V)
1623.6718 1623.7688 -60 1Met-ox 50 64 0 (R)DVSELTGFPEMLGGR(V)
1715.7574 1715.8645 -62 531 545 0 (K) (D)
2034.8627 2034.9197 -28 178 194 0 (K) (K)
2102.9353 2103.0333 -47 1Met-ox 208 225 0 (R)YGMNPHQTPAQLYTLQPK(L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 23% (141/592AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 9/78 matches (11%).
Acc. #: Species: MOUSE Name: Neurofilament triplet M protein (160 kDa neurofilament protein) (Neurofilament medium polypeptide) (NF-M)
Index: MW: 96042 Da pI: 4.8

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
890.4261 890.4219 4.6 196 203 0 (R) (A)
960.4463 960.4162 31 495 502 0 (K) (S)
1065.4734 1065.4965 -22 186 193 1 (K) (L)
1065.4734 1065.5329 -56 314 321 1 (K) (Q)
1158.5143 1158.5907 -66 118 126 0 (K) (E)
1158.5143 1158.6006 -75 656 665 1 (K) (K)
1236.5270 1236.6364 -88 452 461 0 (K) (V)
1273.6146 1273.6276 -10 731 742 0 (K) (E)
1675.7048 1675.7186 -8.3 489 502 1 (K) (S)
2057.8617 2058.0005 -67 137 154 1 (R) (E)
2418.1380 2418.0243 47 274 292 0 (R) (C)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 13% (116/849AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 6/78 matches (7%).
Acc. #: Species: MOUSE Name: Apoptosis regulator Bcl-W (BCL2-like 2 protein)
Index: MW: 20791 Da pI: 5.4

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1163.5582 1163.5367 18 1Met-ox 1 11 0 (-)MATPASTPDTR(A)
1298.5652 1298.6129 -37 48 58 1 (R) (R)
1937.7361 1937.9146 -92 79 95 0 (R) (L)
2026.9094 2027.0310 -60 60 78 0 (R) (F)
2210.9995 2211.1120 -51 1 21 1 (-) (L)
2225.0132 2225.0232 -4.5 26 47 0 (K) (A)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 46% (90/193AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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