MS-Fit Search Results

Parameters

Database searched: SwissProt.01.22.2003
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): 40-27
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 121920
Molecular weight search (1000 - 100000 Da) selects 115277 entries.
Full pI range: 121920 entries.
Species search ( HUMAN MOUSE ) selects 14893 entries.
Combined molecular weight, pI and species searches select 13163 entries.
Pre searches select 13163 entries.

Data Set 1 Results

MS-Fit search selects 608 entries (results displayed for top 5 matches).

Results Summary

	MOWSE Score	#/62(%) Masses Matched	% Cov	% TIC	Mean Err ppm	Data Tol ppm	Protein MW (Da)/pI	Species	Protein Name
1	1.823e+010	25 (40)	37.0	40.3	35.3	31.5	74140/6.6	HUMAN	Lamin A/C (70 kDa lamin)
2	6.950e+008	23 (37)	36.0	37.1	33.7	32.4	74210/6.5	MOUSE	Lamin A
3	2.365e+008	22 (35)	39.0	35.5	33.5	33.1	65447/6.4	MOUSE	Lamins C and C2
4	1.109e+006	11 (17)	21.0	17.7	16.2	55.1	73141/6.7	MOUSE	Putative ATP-dependent RNA helicase PL10
5	1.783e+005	10 (16)	18.0	16.1	13.1	59.6	73102/6.7	MOUSE	DEAD-box protein 3 (DEAD-box RNA helicase DEAD3) (mDEAD3) (Embryonic RNA helicase) (D1PAS1 related sequence 2)

81589	.	.	.	x	x	x	x	.	x	x	.	x	.	x	x	.	.	.	.	x	.	x	.	.	.	x	.	x	x	.	x	x	.	.	.	x	.	x	x	x	x	.	.	x	.	x	.	.	.	.	x	.	.	.	.	.	.	.	.	x	.	.
15157	.	.	.	x	x	x	x	.	x	x	.	x	.	x	x	.	.	.	.	.	.	.	.	.	.	x	.	x	.	.	x	x	.	.	.	x	.	x	x	x	x	.	.	x	x	x	.	.	.	.	x	.	.	.	.	.	.	.	.	x	.	.
118002	.	.	.	x	x	x	x	.	x	x	.	x	.	x	x	.	.	.	.	.	.	.	.	.	.	x	.	x	.	.	x	x	.	.	.	x	.	.	x	x	x	.	.	x	x	x	.	.	.	.	x	.	.	.	.	.	.	.	.	x	.	.
105637	.	.	.	.	.	.	.	.	.	x	x	.	.	.	.	x	.	x	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	x	.	x	x	.	.	x	.	.	.	x	.	.	.	.	.
70323	.	.	.	.	.	.	.	.	.	x	x	.	.	.	.	x	.	.	x	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	x	x	.	.	x	.	.	.	x	.	.	.	.	.

Detailed Results

1. 25/62 matches (40%).
Acc. #: Species: HUMAN Name: Lamin A/C (70 kDa lamin)
Index: MW: 74140 Da pI: 6.6

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
972.5336	972.5114	23		389	397	0	(R) (S)
1023.5415	1023.5111	30		209	216	0	(K) (E)
1028.6037	1028.5740	29		241	249	0	(R) (A)
1043.5876	1043.5485	37		124	133	0	(K) (L)
1048.5429	1048.5097	32	1Met-ox	182	189	0	(K)QLQDEMLR(R)
1089.5943	1089.5540	37		51	60	0	(R) (L)
1165.5995	1165.5489	43		79	89	0	(K) (K)
1182.6591	1182.6119	40		157	166	0	(R) (G)
1187.6730	1187.6384	29		320	329	1	(K) (E)
1274.6592	1274.6017	45	pyroGlu	472	482	0	(R)QNGDDPLLTYR(F)
1291.6867	1291.6283	45		472	482	0	(R) (F)
1347.7285	1347.6731	41	1Met-ox	367	377	0	(K)LALDMEIHAYR(K)
1359.7358	1359.6868	36		12	25	0	(R) (I)
1377.6905	1377.6334	41		516	527	0	(K) (T)
1430.8430	1430.7855	40		299	311	0	(R) (Q)
1475.8181	1475.7681	34	1Met-ox	367	378	1	(K)LALDMEIHAYRK(L)
1507.8059	1507.7426	42	1Met-ox	528	541	0	(R)TALINSTGEEVAMR(K)
1566.8108	1566.7512	38		628	644	0	(R) (S)
1605.8776	1605.8124	41		440	453	1	(R) (L)
1619.8961	1619.8427	33		528	542	1	(R) (L)
1629.8727	1629.8084	39		29	41	1	(R) (L)
1700.0472	1699.9707	45		297	311	1	(R) (Q)
1752.9457	1752.8629	47		281	296	0	(R) (I)
1910.0134	1909.9217	48	1Met-ox	352	366	0	(R)MQQQLDEYQELLDIK(L)
2384.1040	2384.1846	-34		241	260	1	(R) (K)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 37% (250/664AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 23/62 matches (37%).
Acc. #: Species: MOUSE Name: Lamin A
Index: MW: 74210 Da pI: 6.5

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
972.5336 972.5114 23 389 397 0 (R) (S)
1023.5415 1023.5111 30 209 216 0 (K) (E)
1028.6037 1028.5740 29 241 249 0 (R) (A)
1043.5876 1043.5485 37 124 133 0 (K) (L)
1048.5429 1048.5097 32 1Met-ox 182 189 0 (K)QLQDEMLR(R)
1089.5943 1089.5540 37 51 60 0 (R) (L)
1165.5995 1165.5489 43 79 89 0 (K) (K)
1182.6591 1182.6119 40 157 166 0 (R) (G)
1187.6730 1187.6384 29 320 329 1 (K) (E)
1347.7285 1347.6731 41 1Met-ox 367 377 0 (K)LALDMEIHAYR(K)
1359.7358 1359.6868 36 12 25 0 (R) (I)
1430.8430 1430.7855 40 299 311 0 (R) (Q)
1475.8181 1475.7681 34 1Met-ox 367 378 1 (K)LALDMEIHAYRK(L)
1507.8059 1507.7426 42 1Met-ox 528 541 0 (R)TALINSTGEEVAMR(K)
1566.8108 1566.7512 38 629 645 0 (R) (S)
1605.8776 1605.8124 41 440 453 1 (R) (L)
1619.8961 1619.8427 33 528 542 1 (R) (L)
1629.8727 1629.8084 39 29 41 1 (R) (L)
1700.0472 1699.9707 45 297 311 1 (R) (Q)
1707.8459 1707.8050 24 421 435 1 (K) (T)
1752.9457 1752.8629 47 281 296 0 (R) (I)
1910.0134 1909.9217 48 1Met-ox 352 366 0 (R)MQQQLDEYQELLDIK(L)
2384.1040 2384.1846 -34 241 260 1 (R) (K)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 36% (242/665AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 22/62 matches (35%).
Acc. #: Species: MOUSE Name: Lamins C and C2
Index: MW: 65447 Da pI: 6.4

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
972.5336 972.5114 23 389 397 0 (R) (S)
1023.5415 1023.5111 30 209 216 0 (K) (E)
1028.6037 1028.5740 29 241 249 0 (R) (A)
1043.5876 1043.5485 37 124 133 0 (K) (L)
1048.5429 1048.5097 32 1Met-ox 182 189 0 (K)QLQDEMLR(R)
1089.5943 1089.5540 37 51 60 0 (R) (L)
1165.5995 1165.5489 43 79 89 0 (K) (K)
1182.6591 1182.6119 40 157 166 0 (R) (G)
1187.6730 1187.6384 29 320 329 1 (K) (E)
1347.7285 1347.6731 41 1Met-ox 367 377 0 (K)LALDMEIHAYR(K)
1359.7358 1359.6868 36 12 25 0 (R) (I)
1430.8430 1430.7855 40 299 311 0 (R) (Q)
1475.8181 1475.7681 34 1Met-ox 367 378 1 (K)LALDMEIHAYRK(L)
1507.8059 1507.7426 42 1Met-ox 528 541 0 (R)TALINSTGEEVAMR(K)
1605.8776 1605.8124 41 440 453 1 (R) (L)
1619.8961 1619.8427 33 528 542 1 (R) (L)
1629.8727 1629.8084 39 29 41 1 (R) (L)
1700.0472 1699.9707 45 297 311 1 (R) (Q)
1707.8459 1707.8050 24 421 435 1 (K) (T)
1752.9457 1752.8629 47 281 296 0 (R) (I)
1910.0134 1909.9217 48 1Met-ox 352 366 0 (R)MQQQLDEYQELLDIK(L)
2384.1040 2384.1846 -34 241 260 1 (R) (K)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 39% (225/574AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 11/62 matches (17%).
Acc. #: Species: MOUSE Name: Putative ATP-dependent RNA helicase PL10
Index: MW: 73141 Da pI: 6.7

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1089.5943 1089.6057 -10 199 207 0 (R) (H)
1157.6207 1157.5876 29 363 372 0 (R) (G)
1194.6819 1194.6417 34 315 325 0 (R) (L)
1263.7333 1263.7200 11 440 450 0 (K) (K)
1291.6867 1291.7262 -31 428 439 0 (R) (D)
1707.8459 1707.8964 -30 311 325 1 (R) (L)
1765.8217 1765.7741 27 599 615 1 (R) (A)
1839.0137 1838.9513 34 531 547 1 (R) (N)
1882.9717 1882.9187 28 145 161 0 (R) (Y)
1941.0533 1940.9792 38 335 350 1 (K) (M)
2083.1262 2083.0249 49 511 527 0 (K) (I)

m/z Submitted	MH⁺ Matched	Delta ppm	Start	End	Missed Cleavages	Database Sequence
1089.5943	1089.6057	-10	199	207	0	(R) (H)
1157.6207	1157.5876	29	363	372	0	(R) (G)
1194.6819	1194.6417	34	315	325	0	(R) (L)
1263.7333	1263.7200	11	440	450	0	(K) (K)
1291.6867	1291.7262	-31	428	439	0	(R) (D)
1707.8459	1707.8964	-30	311	325	1	(R) (L)
1765.8217	1765.7741	27	599	615	1	(R) (A)
1839.0137	1838.9513	34	531	547	1	(R) (N)
1882.9717	1882.9187	28	145	161	0	(R) (Y)
1941.0533	1940.9792	38	335	350	1	(K) (M)
2083.1262	2083.0249	49	511	527	0	(K) (I)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 21% (141/660AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 10/62 matches (16%).
Acc. #: Species: MOUSE Name: DEAD-box protein 3 (DEAD-box RNA helicase DEAD3) (mDEAD3) (Embryonic RNA helicase) (D1PAS1 related sequence 2)
Index: MW: 73102 Da pI: 6.7

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1089.5943 1089.6057 -10 200 208 0 (R) (H)
1157.6207 1157.5876 29 364 373 0 (R) (G)
1194.6819 1194.6417 34 316 326 0 (R) (L)
1272.6834 1272.6952 -9.3 419 428 1 (K) (S)
1291.6867 1291.7262 -31 429 440 0 (R) (D)
1707.8459 1707.8964 -30 312 326 1 (R) (L)
1839.0137 1838.9513 34 532 548 1 (R) (N)
1882.9717 1882.9187 28 146 162 0 (R) (Y)
1941.0533 1940.9792 38 336 351 1 (K) (M)
2083.1262 2083.0249 49 512 528 0 (K) (I)

m/z Submitted	MH⁺ Matched	Delta ppm	Start	End	Missed Cleavages	Database Sequence
1089.5943	1089.6057	-10	200	208	0	(R) (H)
1157.6207	1157.5876	29	364	373	0	(R) (G)
1194.6819	1194.6417	34	316	326	0	(R) (L)
1272.6834	1272.6952	-9.3	419	428	1	(K) (S)
1291.6867	1291.7262	-31	429	440	0	(R) (D)
1707.8459	1707.8964	-30	312	326	1	(R) (L)
1839.0137	1838.9513	34	532	548	1	(R) (N)
1882.9717	1882.9187	28	146	162	0	(R) (Y)
1941.0533	1940.9792	38	336	351	1	(K) (M)
2083.1262	2083.0249	49	512	528	0	(K) (I)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 18% (123/662AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):