MS-Fit Search Results


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Parameters

Database searched: SwissProt.01.22.2003
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): 40-29
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 121920
Molecular weight search (1000 - 100000 Da) selects 115277 entries.
Full pI range: 121920 entries.
Species search ( HUMAN MOUSE ) selects 14893 entries.
Combined molecular weight, pI and species searches select 13163 entries.
Pre searches select 13163 entries.

Data Set 1 Results

MS-Fit search selects 1673 entries (results displayed for top 10 matches).

Results Summary

MOWSE
Score		#/90(%)
Masses
Matched 		%
Cov		%
TIC		Mean
Err
ppm		Data
Tol
ppm		MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
11.628e+00913 (14)37.014.432.442.6 50152/4.9HUMANTubulin alpha-1 chain (Alpha-tubulin 1)
23.422e+00812 (13)34.013.331.744.2 49896/5.0HUMANTubulin alpha-6 chain (Alpha-tubulin 6)
33.421e+00812 (13)34.013.331.744.2 49910/5.0MOUSETubulin alpha-6 chain (Alpha-tubulin 6)
42.940e+00812 (13)34.013.331.744.2 50166/4.9MOUSETubulin alpha-2 chain (Alpha-tubulin 2)
56.406e+00711 (12)31.012.229.643.5 49925/4.9HUMANTubulin alpha-4 chain (Alpha-tubulin 4)
66.292e+00711 (12)31.012.232.246.2 50136/4.9MOUSETubulin alpha-1 chain
72.945e+00711 (12)32.012.232.156.7 49960/5.0MMOUSETubulin alpha-3/alpha-7 chain (Alpha-tubulin 3/7)
85.209e+0058 (8)21.08.931.736.4 50052/5.0MOUSETubulin alpha-8 chain (Alpha-tubulin 8)
95.204e+0059 (10)24.010.035.039.5 50094/4.9HUMANTubulin alpha-8 chain (Alpha-tubulin 8)
101.913e+00514 (15)35.015.633.436.6 54151/7.6HUMANDihydrolipoamide dehydrogenase, mitochondrial precursor
24771.....x...........x..............xx......x........x......x...x.x....x......x.........xx....
17061.....x...........x..............xx......x...............x...x.x....x......x.........xx....
93507.....x...........x..............xx......x...............x...x.x....x......x.........xx....
15574.....x...........x..............xx......x...............x...x.x....x......x.........xx....
71088.....x..........................xx......x........x.........xx.x....x................xx....
107553.....x...........x..............xx......................x...x.x....x......x.........xx....
74622.....x.......................x..xx....................x.....x.x....x......x.........xx....
741................................xx......x...................x...x..x................xx....
99816................................xx......x...............x...x...x..x................xx....
49995.....x.x..x....x..x..x.....................x..xx.............x......x...xx............x...


Detailed Results

1. 13/90 matches (14%).
Acc. #: Species: HUMAN Name: Tubulin alpha-1 chain (Alpha-tubulin 1)
Index: MW: 50152 Da pI: 4.9
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
909.4851909.5046-211571641(R) (S)
1085.65361085.6206301131210(K) (I)
1393.77711393.748021pyroGlu 85960(R)QLFHPEQLITGK(E)
1410.81841410.77453185960(R) (E)
1487.91931487.8797272302430(R) (F)
1598.83261598.7677413403520(R) (V)
1701.97841701.90634265790(R) (T)
1718.96091718.8826462162290(R) (L)
1757.01831756.9638312652800(R) (A)
1825.01861824.9860183533700(K) (V)
2008.00512007.89365641600(K) (H)
2409.28772409.2091332442640(R) (I)
2415.37072415.205768851051(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 37% (169/451AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 12/90 matches (13%).
Acc. #: Species: HUMAN Name: Tubulin alpha-6 chain (Alpha-tubulin 6)
Index: MW: 49896 Da pI: 5.0
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
909.4851909.5046-211571641(R) (S)
1085.65361085.6206301131210(K) (I)
1393.77711393.748021pyroGlu 85960(R)QLFHPEQLITGK(E)
1410.81841410.77453185960(R) (E)
1487.91931487.8797272302430(R) (F)
1701.97841701.90634265790(R) (T)
1718.96091718.8826462162290(R) (L)
1757.01831756.9638312652800(R) (A)
1825.01861824.9860183533700(K) (V)
2008.00512007.89365641600(K) (H)
2409.28772409.2091332442640(R) (I)
2415.37072415.205768851051(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 34% (156/449AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 12/90 matches (13%).
Acc. #: Species: MOUSE Name: Tubulin alpha-6 chain (Alpha-tubulin 6)
Index: MW: 49910 Da pI: 5.0
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
909.4851909.5046-211571641(R) (S)
1085.65361085.6206301131210(K) (I)
1393.77711393.748021pyroGlu 85960(R)QLFHPEQLITGK(E)
1410.81841410.77453185960(R) (E)
1487.91931487.8797272302430(R) (F)
1701.97841701.90634265790(R) (T)
1718.96091718.8826462162290(R) (L)
1757.01831756.9638312652800(R) (A)
1825.01861824.9860183533700(K) (V)
2008.00512007.89365641600(K) (H)
2409.28772409.2091332442640(R) (I)
2415.37072415.205768851051(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 34% (156/449AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 12/90 matches (13%).
Acc. #: Species: MOUSE Name: Tubulin alpha-2 chain (Alpha-tubulin 2)
Index: MW: 50166 Da pI: 4.9
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
909.4851909.5046-211571641(R) (S)
1085.65361085.6206301131210(K) (I)
1393.77711393.748021pyroGlu 85960(R)QLFHPEQLITGK(E)
1410.81841410.77453185960(R) (E)
1487.91931487.8797272302430(R) (F)
1701.97841701.90634265790(R) (T)
1718.96091718.8826462162290(R) (L)
1757.01831756.9638312652800(R) (A)
1825.01861824.9860183533700(K) (V)
2008.00512007.89365641600(K) (H)
2409.28772409.2091332442640(R) (I)
2415.37072415.205768851051(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 34% (156/451AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 11/90 matches (12%).
Acc. #: Species: HUMAN Name: Tubulin alpha-4 chain (Alpha-tubulin 4)
Index: MW: 49925 Da pI: 4.9
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
909.4851909.5046-211571641(R) (S)
1393.77711393.748021pyroGlu 85960(R)QLFHPEQLITGK(E)
1410.81841410.77453185960(R) (E)
1487.91931487.8797272302430(R) (F)
1598.83261598.7677413403520(R) (V)
1715.97781715.92203365790(R) (N)
1718.96091718.8826462162290(R) (L)
1757.01831756.9638312652800(R) (A)
1825.01861824.9860183533700(K) (V)
2409.28772409.2091332442640(R) (I)
2415.37072415.205768851051(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 31% (140/448AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

6. 11/90 matches (12%).
Acc. #: Species: MOUSE Name: Tubulin alpha-1 chain
Index: MW: 50136 Da pI: 4.9
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
909.4851909.5046-211571641(R) (S)
1085.65361085.6206301131210(K) (I)
1393.77711393.748021pyroGlu 85960(R)QLFHPEQLITGK(E)
1410.81841410.77453185960(R) (E)
1701.97841701.90634265790(R) (T)
1718.96091718.8826462162290(R) (L)
1757.01831756.9638312652800(R) (A)
1825.01861824.9860183533700(K) (V)
2008.00512007.89365641600(K) (H)
2409.28772409.2091332442640(R) (I)
2415.37072415.205768851051(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 31% (142/451AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

7. 11/90 matches (12%).
Acc. #: Species: MOUSE Name: Tubulin alpha-3/alpha-7 chain (Alpha-tubulin 3/7)
Acc. #: Species: HUMAN Name: Tubulin alpha-2 chain (Alpha-tubulin 2)
Index: MW: 49960 Da pI: 5.0
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
909.4851909.5046-211571641(R) (S)
1340.78831340.7902-1.41131231(K) (K)
1393.77711393.748021pyroGlu 85960(R)QLFHPEQLITGK(E)
1410.81841410.77453185960(R) (E)
1688.01421687.89077365790(R) (T)
1718.96091718.8826462162290(R) (L)
1757.01831756.9638312652800(R) (A)
1825.01861824.9860183533700(K) (V)
2008.00512007.89365641600(K) (H)
2409.28772409.2091332442640(R) (I)
2415.37072415.205768851051(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 32% (144/450AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

8. 8/90 matches (8%).
Acc. #: Species: MOUSE Name: Tubulin alpha-8 chain (Alpha-tubulin 8)
Index: MW: 50052 Da pI: 5.0
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1393.77711393.748021pyroGlu 85960(R)QLFHPEQLITGK(E)
1410.81841410.77453185960(R) (E)
1487.91931487.8797272302430(R) (F)
1718.96091718.8826462162290(R) (L)
1765.84441765.825811971121(K) (E)
1825.01861824.9860183533700(K) (V)
2409.28772409.2091332442640(R) (I)
2415.37072415.205768851051(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 21% (95/449AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

9. 9/90 matches (10%).
Acc. #: Species: HUMAN Name: Tubulin alpha-8 chain (Alpha-tubulin 8)
Index: MW: 50094 Da pI: 4.9
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1393.77711393.748021pyroGlu 85960(R)QLFHPEQLITGK(E)
1410.81841410.77453185960(R) (E)
1487.91931487.8797272302430(R) (F)
1701.97841701.8733621Met-ox 65790(R)AVMIDLEPTVVDEVR(A)
1718.96091718.8826462162290(R) (L)
1765.84441765.825811971121(K) (E)
1825.01861824.9860183533700(K) (V)
2409.28772409.2091332442640(R) (I)
2415.37072415.205768851051(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 24% (110/449AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

10. 14/90 matches (15%).
Acc. #: Species: HUMAN Name: Dihydrolipoamide dehydrogenase, mitochondrial precursor
Index: MW: 54151 Da pI: 7.6
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
909.4851909.4583294214280(K) (A)
912.5456912.53798.43353421(R) (F)
936.4632936.4461181Met-ox 1101170(R)GIEMSEVR(L)
1007.51341007.5162-2.84965050(R) (S)
1127.68501127.6577241331430(K) (Q)
1193.69061193.6431404184281(K) (A)
1538.81671538.7538414834950(R) (E)
1567.88121567.8332313213340(K) (G)
1581.88031581.7648734054171(K) (V)
1746.00791745.9294453013150(K) (R)
1847.97231847.89953973890(K) (A)
1986.17251986.0846441Met-ox 2162340(K)MVVIGAGVIGVELGSVWQR(L)
1995.13721995.0737321Met-ox 3473650(K)IPNIYAIGDVVAGPMLAHK(A)
2530.40022530.2863451Met-ox 2352590(R)LGADVTAVEFLGHVGGVGIDMEISK(N)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 35% (183/509AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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