MS-Fit Search Results

MS-Fit Search Results

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Parameters

Database searched: SwissProt.01.22.2003
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): 40-30
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 121920
Molecular weight search (1000 - 100000 Da) selects 115277 entries.
Full pI range: 121920 entries.
Species search ( HUMAN MOUSE ) selects 14893 entries.
Combined molecular weight, pI and species searches select 13163 entries.
Pre searches select 13163 entries.

Data Set 1 Results

MS-Fit search selects 367 entries (results displayed for top 5 matches).

Results Summary

	MOWSE Score	#/60(%) Masses Matched	% Cov	% TIC	Mean Err ppm	Data Tol ppm	MS-Digest Index #	Protein MW (Da)/pI	Accession #	Species	Protein Name
1	2.367e+005	16 (26)	36.0	26.7	-24.0	25.4		61398/7.7		HUMAN	Glutamate dehydrogenase 1, mitochondrial precursor (GDH)
2	5.186e+004	15 (25)	33.0	25.0	-19.5	39.4		61337/8.1		MOUSE	Glutamate dehydrogenase, mitochondrial precursor (GDH)
3	4.344e+004	11 (18)	24.0	18.3	-20.1	35.0		61435/8.6		HUMAN	Glutamate dehydrogenase 2, mitochondrial precursor (GDH)
4	2600	4 (6)	13.0	6.7	-24.8	11.6		59751/9.2		HUMAN	ATP synthase alpha chain, mitochondrial precursor
5	2223	4 (6)	13.0	6.7	16.1	88.7		63058/5.3		MOUSE	Glypican-6 precursor

71065	.	x	x	.	.	x	.	.	.	.	.	.	x	.	.	x	x	.	.	.	x	x	.	.	.	.	.	x	.	x	.	.	.	.	.	.	.	x	x	x	.	x	.	.	.	.	x	.	.	.	.	x	.	.	.	.	.	.	.	.
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53427	.	x	x	.	.	x	.	.	.	.	.	.	x	.	.	.	.	.	.	.	x	x	.	.	.	.	.	.	.	x	.	.	.	x	.	.	.	x	.	.	.	x	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.
76648	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	x	.	.	.	.
76297	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	x	.	x	.	.	.	.	.	.	.	x	.	.

Detailed Results

1. 16/60 matches (26%).
Acc. #: Species: HUMAN Name: Glutamate dehydrogenase 1, mitochondrial precursor (GDH)
Index: MW: 61398 Da pI: 7.7

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
963.4962	963.5264	-31		528	535	0	(K) (T)
1016.4188	1016.4511	-32	1Met-ox	69	76	0	(K)MVEGFFDR(G)
1059.4969	1059.5336	-35		445	453	0	(K) (L)
1425.5991	1425.6286	-21		125	136	0	(R) (A)
1507.6716	1507.7215	-33	1Met-ox	504	516	0	(K)DIVHSGLAYTMER(S)
1543.7862	1543.8332	-30		77	90	1	(R) (T)
1581.7002	1581.7298	-19		124	136	1	(R) (A)
1624.8494	1624.8182	19		148	162	1	(K) (A)
1737.8456	1737.8924	-27		481	496	0	(K) (I)
1764.8386	1764.8855	-27	1Met-ox	303	318	0	(K)TFVVQGFGNVGLHSMR(Y)
1908.0436	1908.0893	-24		108	123	0	(R) (R)
1931.8824	1931.9173	-18	1Met-ox	213	231	0	(K)GFIGPGIDVPAPDMSTGER(E)
1936.8568	1936.9075	-26	1Met-ox	461	476	0	(R)DSNYHLLMSVQESLER(K)
1957.9717	1958.0387	-34		347	363	1	(K) (A)
2059.9677	2060.0123	-22	1Met-ox	212	231	1	(K)KGFIGPGIDVPAPDMSTGER(E)
2242.1155	2242.1719	-25		400	420	1	(K) (N)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 36% (202/558AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 15/60 matches (25%).
Acc. #: Species: MOUSE Name: Glutamate dehydrogenase, mitochondrial precursor (GDH)
Index: MW: 61337 Da pI: 8.1

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
963.4962 963.5264 -31 528 535 0 (K) (T)
1016.4188 1016.4511 -32 1Met-ox 69 76 0 (K)MVEGFFDR(G)
1059.4969 1059.5336 -35 445 453 0 (K) (L)
1425.5991 1425.6286 -21 125 136 0 (R) (A)
1507.6716 1507.7215 -33 1Met-ox 504 516 0 (K)DIVHSGLAYTMER(S)
1581.7002 1581.7298 -19 124 136 1 (R) (A)
1624.8494 1624.8182 19 148 162 1 (K) (A)
1764.8386 1764.8855 -27 1Met-ox 303 318 0 (K)TFVVQGFGNVGLHSMR(Y)
1908.0436 1908.0893 -24 108 123 0 (R) (R)
1931.8824 1931.9173 -18 1Met-ox 213 231 0 (K)GFIGPGIDVPAPDMSTGER(E)
1936.8568 1936.9075 -26 1Met-ox 461 476 0 (R)DSNYHLLMSVQESLER(K)
1957.9717 1958.0387 -34 347 363 1 (K) (A)
2011.9471 2011.8786 34 52 68 1 (R) (M)
2059.9677 2060.0123 -22 1Met-ox 212 231 1 (K)KGFIGPGIDVPAPDMSTGER(E)
2242.1155 2242.1719 -25 400 420 1 (K) (N)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 33% (189/558AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 11/60 matches (18%).
Acc. #: Species: HUMAN Name: Glutamate dehydrogenase 2, mitochondrial precursor (GDH)
Index: MW: 61435 Da pI: 8.6

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
963.4962 963.5264 -31 528 535 0 (K) (T)
1016.4188 1016.4511 -32 1Met-ox 69 76 0 (K)MVEGFFDR(G)
1059.4969 1059.5336 -35 445 453 0 (K) (L)
1425.5991 1425.6286 -21 125 136 0 (R) (A)
1581.7002 1581.7298 -19 124 136 1 (R) (A)
1624.8494 1624.8182 19 148 162 1 (K) (A)
1764.8386 1764.8855 -27 1Met-ox 303 318 0 (K)TFVVQGFGNVGLHSMR(Y)
1819.9259 1819.9125 7.3 504 519 1 (K) (Q)
1908.0436 1908.0893 -24 108 123 0 (R) (R)
1957.9717 1958.0387 -34 347 363 1 (K) (A)
2242.1155 2242.1719 -25 400 420 1 (K) (N)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 24% (139/558AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 4/60 matches (6%).
Acc. #: Species: HUMAN Name: ATP synthase alpha chain, mitochondrial precursor
Index: MW: 59751 Da pI: 9.2

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1553.7076 1553.7389 -20 335 347 0 (R) (L)
1624.8494 1624.8910 -26 134 149 0 (R) (V)
2338.1196 2338.1679 -21 442 463 0 (R) (G)
2367.1801 2367.2573 -33 507 527 0 (K) (A)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 13% (72/553AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 4/60 matches (6%).
Acc. #: Species: MOUSE Name: Glypican-6 precursor
Index: MW: 63058 Da pI: 5.3

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1908.0436 1907.9537 47 111 126 1 (R) (T)
2064.0865 2064.0193 33 259 275 0 (R) (G)
2211.0570 2211.1670 -50 534 552 0 (K) (L)
2662.3235 2662.2322 34 172 192 0 (R) (Y)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 13% (73/555AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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