MS-Fit Search Results


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Parameters

Database searched: SwissProt.01.22.2003
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): 40-31
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 121920
Molecular weight search (1000 - 100000 Da) selects 115277 entries.
Full pI range: 121920 entries.
Species search ( HUMAN MOUSE ) selects 14893 entries.
Combined molecular weight, pI and species searches select 13163 entries.
Pre searches select 13163 entries.

Data Set 1 Results

MS-Fit search selects 560 entries (results displayed for top 5 matches).

Results Summary

MOWSE
Score		#/59(%)
Masses
Matched 		%
Cov		%
TIC		Mean
Err
ppm		Data
Tol
ppm		MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
14.648e+00718 (30)38.030.53.2534.6 60188/6.7HUMANHeterogeneous nuclear ribonucleoprotein L (hnRNP L)
28.550e+0046 (10)10.010.219.848.2 27923/8.7MOUSEB-cell receptor-associated protein 31 (BCR-associated protein Bap31) (p28 Bap31)
38.529e+0046 (10)10.010.219.848.2 27992/8.4HUMANB-cell receptor-associated protein 31 (BCR-associated protein Bap31) (p28 Bap31) (CDM protein) (6C6-AG tumor-associated antigen) (DXS1357E)
43.133e+0047 (11)17.011.916.228.1 73244/6.7HUMANDEAD-box protein 3 (Helicase-like protein 2) (HLP2) (DEAD-box, X isoform)
52.451e+0048 (13)23.013.61.5541.8 66018/8.2HUMANKeratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
4254.xxxx.xx..xx...........xx....x......xxx.x.....x..........xx
117588....................................x.xx...........xx...x..
108086....................................x.xx...........xx...x..
3614.........x...................x....x...x....x....x.....x....
48753........x....x....x.......xx.........x........x......x.....


Detailed Results

1. 18/59 matches (30%).
Acc. #: Species: HUMAN Name: Heterogeneous nuclear ribonucleoprotein L (hnRNP L)
Index: MW: 60188 Da pI: 6.7
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
923.4186923.4521-361Met-ox 3143220(R)MGPPVGGHR(R)
977.5245977.5420-182342410(K) (L)
978.4777978.4896-124544620(R) (N)
1002.42431002.4281-3.816250(R) (A)
1076.61541076.6217-5.867760(K) (G)
1079.54581079.5533-6.91Met-ox 3143231(R)MGPPVGGHRR(G)
1079.54581079.5533-6.91Met-ox 3133221(R)RMGPPVGGHR(R)
1222.59411222.5956-1.25115210(R) (S)
1263.64011263.63742.25385480(K) (L)
1602.80651602.79904.73683800(R) (V)
1650.82841650.8062131Met-ox 4044170(R)AITHLNNNFMFGQK(L)
1757.84831757.8202162182330(K) (I)
1866.89671866.9060-5.05225370(K) (N)
1881.92261881.8765251Met-ox 1992150(K)NGVQAMVEFDSVQSAQR(A)
1882.90901882.90094.31Met-ox 5225370(K)SDALETLGFLNHYQMK(N)
1899.89521899.8581202Met-ox 3864030(K)SKPGAAMVEMADGYAVDR(A)
1993.99801993.9765111982151(R) (A)
2890.33362890.2628242472721(K) (Q)
2906.37162906.261338pyroGlu 4254501(K)QPAIMPGQSYGLEDGSCSYKDFSESR(N)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 38% (217/558AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 6/59 matches (10%).
Acc. #: Species: MOUSE Name: B-cell receptor-associated protein 31 (BCR-associated protein Bap31) (p28 Bap31)
Index: MW: 27923 Da pI: 8.7
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1866.89671866.87431280950(K) (L)
1882.90901882.8692211Met-ox 80950(K)VNLQNNPGAMEHFHMK(L)
1898.90021898.8642192Met-ox 80950(K)VNLQNNPGAMEHFHMK(L)
2283.22342283.12794280981(K) (A)
2299.22852299.1228461Met-ox 80981(K)VNLQNNPGAMEHFHMKLFR(A)
2717.18802717.2452-2173951(K) (L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 10% (26/245AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 6/59 matches (10%).
Acc. #: Species: HUMAN Name: B-cell receptor-associated protein 31 (BCR-associated protein Bap31) (p28 Bap31) (CDM protein) (6C6-AG tumor-associated antigen) (DXS1357E)
Index: MW: 27992 Da pI: 8.4
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1866.89671866.87431280950(K) (L)
1882.90901882.8692211Met-ox 80950(K)VNLQNNPGAMEHFHMK(L)
1898.90021898.8642192Met-ox 80950(K)VNLQNNPGAMEHFHMK(L)
2283.22342283.12794280981(K) (A)
2299.22852299.1228461Met-ox 80981(K)VNLQNNPGAMEHFHMKLFR(A)
2717.18802717.2452-2173951(K) (L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 10% (26/246AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 7/59 matches (11%).
Acc. #: Species: HUMAN Name: DEAD-box protein 3 (Helicase-like protein 2) (HLP2) (DEAD-box, X isoform)
Index: MW: 73244 Da pI: 6.7
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1194.68091194.6417333163260(R) (L)
1757.84831757.7982281311450(K) (L)
1838.97981838.9513155325481(R) (N)
1882.90901882.9187-5.11461620(R) (Y)
1940.97921940.97920.00623363511(K) (M)
2083.06802083.0249215125280(K) (I)
2662.48392662.4278212312551(K) (E)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 17% (118/662AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 8/59 matches (13%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
Index: MW: 66018 Da pI: 8.2
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1179.60771179.60105.73773860(K) (H)
1277.66711277.6337262472571(K) (N)
1277.66711277.7105-344734830(K) (T)
1475.76691475.7494122122230(K) (T)
1475.76691475.7858-132002110(R) (W)
1653.80691653.8158-5.42903041(K) (L)
1687.98661687.97079.466821(R) (G)
1881.92261881.9711-261821971(R) (V)
1993.99801993.9772102242390(R) (R)
2384.02372383.9524305195490(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 23% (151/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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