MS-Fit Search Results

MS-Fit Search Results

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Parameters

Database searched: SwissProt.01.22.2003
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): 40-32
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 121920
Molecular weight search (1000 - 100000 Da) selects 115277 entries.
Full pI range: 121920 entries.
Species search ( HUMAN MOUSE ) selects 14893 entries.
Combined molecular weight, pI and species searches select 13163 entries.
Pre searches select 13163 entries.

Data Set 1 Results

MS-Fit search selects 453 entries (results displayed for top 5 matches).

Results Summary

	MOWSE Score	#/59(%) Masses Matched	% Cov	% TIC	Mean Err ppm	Data Tol ppm	MS-Digest Index #	Protein MW (Da)/pI	Accession #	Species	Protein Name
1	5.383e+006	12 (20)	31.0	20.3	2.71	39.8		59757/6.9		HUMAN	Catalase
2	2.539e+006	10 (16)	25.0	16.9	6.54	44.3		66018/8.2		HUMAN	Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
3	5.674e+004	9 (15)	26.0	15.3	8.37	39.2		57937/8.0		HUMAN	Pyruvate kinase, M1 isozyme (Pyruvate kinase muscle isozyme) (Cytosolic thyroid hormone-binding protein) (CTHBP) (THBP1)
4	1.982e+004	8 (13)	25.0	13.6	5.39	37.3		57914/8.0		HUMAN	Pyruvate kinase, M2 isozyme
5	9124	6 (10)	9.0	10.2	4.64	39.0		80509/5.5		HUMAN	Adseverin (Scinderin)

39951	.	x	.	.	x	.	.	x	.	.	.	.	.	.	x	x	.	.	.	x	.	.	x	x	.	.	.	.	x	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	x	.	.	.	.	.
48753	.	.	.	.	.	x	.	.	x	.	.	.	.	x	.	.	.	.	.	.	x	.	.	.	.	.	x	.	.	.	.	.	x	.	.	.	.	.	.	x	.	.	.	.	x	.	.	.	.	.	.	.	x	.	x	.	.	.	.
48470	x	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	x	.	x	.	x	.	.	.	.	.	.	x	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.
9907	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	x	.	x	.	x	.	.	.	.	.	.	x	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.
11560	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	x	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	x	.	.	.	x	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.

Detailed Results

1. 12/59 matches (20%).
Acc. #: Species: HUMAN Name: Catalase
Index: MW: 59757 Da pI: 6.9

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
968.5466	968.5893	-44		39	47	0	(K) (G)
1119.5353	1119.5587	-21		355	363	0	(R) (H)
1292.6164	1292.6123	3.2		253	263	0	(R) (D)
1481.7377	1481.7389	-0.80		445	456	0	(R) (K)
1493.7266	1493.6985	19		432	444	0	(R) (A)
1648.9280	1648.9215	3.9		302	315	1	(K) (L)
1708.8731	1708.8883	-8.9		507	522	0	(K) (E)
1712.8421	1712.8284	8.0		78	93	0	(K) (Y)
1826.9591	1826.9376	12		366	380	0	(R) (A)
1851.9108	1851.8837	15		113	130	1	(R) (G)
2205.1023	2205.0650	17	1Met-ox	48	66	0	(R)GPLLVQDVVFTDEMAHFDR(E)
2364.3596	2364.2887	30		24	47	1	(K) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 31% (167/527AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 10/59 matches (16%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
Index: MW: 66018 Da pI: 8.2

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1179.6013 1179.6010 0.30 377 386 0 (K) (H)
1302.6994 1302.7018 -1.8 393 403 1 (R) (V)
1302.6994 1302.7157 -13 344 355 0 (R) (A)
1475.7606 1475.7494 7.5 212 223 0 (K) (T)
1475.7606 1475.7858 -17 200 211 0 (R) (W)
1687.9831 1687.9707 7.4 66 82 1 (R) (G)
1779.8331 1779.7509 46 46 65 0 (R) (S)
1881.9122 1881.9711 -31 182 197 1 (R) (V)
1994.0459 1993.9772 34 224 239 0 (R) (R)
2150.1255 2150.0783 22 224 240 1 (R) (R)
2300.1974 2300.1985 -0.52 344 364 1 (R) (S)
2384.0094 2383.9524 24 519 549 0 (R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 25% (167/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 9/59 matches (15%).
Acc. #: Species: HUMAN Name: Pyruvate kinase, M1 isozyme (Pyruvate kinase muscle isozyme) (Cytosolic thyroid hormone-binding protein) (CTHBP) (THBP1)
Index: MW: 57937 Da pI: 8.0

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
868.5107 868.4827 32 377 383 0 (R) (E)
1373.6930 1373.7211 -20 44 56 0 (R) (S)
1779.8331 1779.8765 -24 189 206 0 (K) (K)
1837.9581 1837.9118 25 1Met-ox 279 294 1 (R)RFDEILEASDGIMVAR(G)
1875.9033 1875.8951 4.4 1Met-ox 231 246 0 (K)FGVEQDVDMVFASFIR(K)
1883.9317 1883.9040 15 74 89 0 (R) (N)
2011.9965 2011.9621 17 152 166 1 (K) (V)
2088.0985 2088.0878 5.1 384 400 1 (R) (L)
2465.3459 2465.2928 22 93 115 0 (R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 26% (141/531AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 8/59 matches (13%).
Acc. #: Species: HUMAN Name: Pyruvate kinase, M2 isozyme
Index: MW: 57914 Da pI: 8.0

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1373.6930 1373.7211 -20 44 56 0 (R) (S)
1779.8331 1779.8765 -24 189 206 0 (K) (K)
1837.9581 1837.9118 25 1Met-ox 279 294 1 (R)RFDEILEASDGIMVAR(G)
1875.9033 1875.8951 4.4 1Met-ox 231 246 0 (K)FGVEQDVDMVFASFIR(K)
1883.9317 1883.9040 15 74 89 0 (R) (N)
2011.9965 2011.9621 17 152 166 1 (K) (V)
2088.0985 2088.0878 5.1 384 400 1 (R) (L)
2465.3459 2465.2928 22 93 115 0 (R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 25% (134/531AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 6/59 matches (10%).
Acc. #: Species: HUMAN Name: Adseverin (Scinderin)
Index: MW: 80509 Da pI: 5.5

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1493.7266 1493.7276 -0.70 150 162 0 (R) (G)
1708.8731 1708.8758 -1.6 448 462 0 (R) (S)
1837.9581 1837.9772 -10 119 137 0 (K) (R)
1994.0459 1994.0783 -16 119 138 1 (K) (L)
2097.0383 2096.9889 24 598 615 0 (K) (L)
2225.1577 2225.0839 33 597 615 1 (K) (L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 9% (67/715AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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