MS-Fit Search Results

MS-Fit Search Results

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Parameters

Database searched: SwissProt.01.22.2003
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): 40-33
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 121920
Molecular weight search (1000 - 100000 Da) selects 115277 entries.
Full pI range: 121920 entries.
Species search ( HUMAN MOUSE ) selects 14893 entries.
Combined molecular weight, pI and species searches select 13163 entries.
Pre searches select 13163 entries.

Data Set 1 Results

MS-Fit search selects 811 entries (results displayed for top 5 matches).

Results Summary

	MOWSE Score	#/60(%) Masses Matched	% Cov	% TIC	Mean Err ppm	Data Tol ppm	MS-Digest Index #	Protein MW (Da)/pI	Accession #	Species	Protein Name
1	4.030e+005	13 (21)	25.0	21.7	39.9	43.5		59367/7.6		HUMAN	T-complex protein 1, eta subunit (TCP-1-eta) (CCT-eta) (HIV-1 Nef interacting protein)
2	6792	7 (11)	10.0	11.7	15.5	83.5		75493/7.6		HUMAN	Thymopoietin, isoform alpha (TP alpha)
3	5143	7 (11)	18.0	11.7	38.4	46.9		49896/5.0		HUMAN	Tubulin alpha-6 chain (Alpha-tubulin 6)
4	5142	7 (11)	18.0	11.7	38.4	46.9		49910/5.0		MOUSE	Tubulin alpha-6 chain (Alpha-tubulin 6)
5	3952	6 (10)	16.0	10.0	18.8	91.1		45469/8.5		MOUSE	Dematin (Erythrocyte membrane protein band 4.9)

18972	.	.	.	.	.	.	x	.	.	.	.	.	x	x	.	.	.	.	.	.	x	x	x	.	.	.	.	.	.	.	x	.	x	.	.	.	.	.	.	.	.	x	x	.	.	.	.	.	x	.	.	.	x	x	.	.	.	.	.	.
56906	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	x	.	.	x	.	.	.	x	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	x	.	.	.	.	.	.	.	.	.	.	.	.	.
17061	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	x	.	x	.	.	.	.	.	.	x	.	.	.	.	.	x	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.
93507	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	x	.	x	.	.	.	.	.	.	x	.	.	.	.	.	x	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.
22370	.	.	.	.	.	.	.	x	.	.	x	.	.	.	.	x	.	.	.	.	.	.	.	.	.	x	.	.	.	x	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.

Detailed Results

1. 13/60 matches (21%).
Acc. #: Species: HUMAN Name: T-complex protein 1, eta subunit (TCP-1-eta) (CCT-eta) (HIV-1 Nef interacting protein)
Index: MW: 59367 Da pI: 7.6

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
924.5114	924.4977	15		369	375	0	(K) (G)
985.5398	985.5318	8.1		314	321	1	(R) (T)
1045.5959	1045.5278	65		248	256	1	(K) (V)
1045.5959	1045.5278	65		525	535	0	(R) (G)
1147.5780	1147.5246	46		358	366	0	(R) (A)
1170.6633	1170.6305	28	1Met-ox	388	397	0	(R)SLHDAIMIVR(R)
1310.7320	1310.7367	-3.6		388	398	1	(R) (A)
1399.6605	1399.5800	58	1Met-ox	376	387	0	(R)GGAEQFMEETER(S)
1421.7024	1421.6411	43	1Met-ox	219	230	0	(K)TFSYAGFEMQPK(K)
1565.8691	1565.7964	46		293	306	0	(K) (D)
1566.8114	1566.7222	57		345	357	0	(R) (Y)
1789.0273	1788.9496	43		200	217	0	(K) (K)
2002.1692	2002.1126	28	pyroGlu	107	123	0	(K)QVKPYVEEGLHPQIIIR(A)
2019.2588	2019.1391	59		107	123	0	(K) (A)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 25% (141/543AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 7/60 matches (11%).
Acc. #: Species: HUMAN Name: Thymopoietin, isoform alpha (TP alpha)
Index: MW: 75493 Da pI: 7.6

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1067.5828 1067.6213 -36 611 620 0 (R) (T)
1085.6452 1085.6206 23 526 535 0 (R) (V)
1170.6633 1170.5941 59 335 344 0 (K) (S)
1330.7464 1330.7119 26 127 139 0 (K) (K)
1350.7504 1350.6694 60 417 427 0 (K) (K)
1718.9616 1718.9230 22 35 48 0 (K) (N)
1774.8896 1774.9703 -45 520 535 1 (K) (V)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 10% (74/694AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 7/60 matches (11%).
Acc. #: Species: HUMAN Name: Tubulin alpha-6 chain (Alpha-tubulin 6)
Index: MW: 49896 Da pI: 5.0

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
909.5764 909.5046 79 157 164 1 (R) (S)
1085.6452 1085.6206 23 113 121 0 (K) (I)
1396.7013 1396.6935 5.6 1Met-ox 391 401 1 (R)LDHKFDLMYAK(R)
1410.8154 1410.7745 29 85 96 0 (R) (E)
1487.9328 1487.8797 36 230 243 0 (R) (F)
1701.9933 1701.9063 51 65 79 0 (R) (T)
1718.9616 1718.8826 46 216 229 0 (R) (L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 18% (83/449AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 7/60 matches (11%).
Acc. #: Species: MOUSE Name: Tubulin alpha-6 chain (Alpha-tubulin 6)
Index: MW: 49910 Da pI: 5.0

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
909.5764 909.5046 79 157 164 1 (R) (S)
1085.6452 1085.6206 23 113 121 0 (K) (I)
1396.7013 1396.6935 5.6 1Met-ox 391 401 1 (R)LDHKFDLMYAK(R)
1410.8154 1410.7745 29 85 96 0 (R) (E)
1487.9328 1487.8797 36 230 243 0 (R) (F)
1701.9933 1701.9063 51 65 79 0 (R) (T)
1718.9616 1718.8826 46 216 229 0 (R) (L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 18% (83/449AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 6/60 matches (10%).
Acc. #: Species: MOUSE Name: Dematin (Erythrocyte membrane protein band 4.9)
Index: MW: 45469 Da pI: 8.5

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
953.4932 953.4692 25 287 295 0 (K) (T)
975.5303 975.4747 57 151 160 0 (R) (H)
1067.5828 1067.5196 59 34 42 0 (K) (D)
1330.7464 1330.6854 46 20 33 0 (R) (M)
1396.7013 1396.7688 -48 161 172 0 (K) (F)
1399.6605 1399.6970 -26 274 286 0 (R) (S)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 16% (67/405AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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