MS-Fit Search Results


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Parameters

Database searched: SwissProt.01.22.2003
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): 40-35
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 121920
Molecular weight search (1000 - 100000 Da) selects 115277 entries.
Full pI range: 121920 entries.
Species search ( HUMAN MOUSE ) selects 14893 entries.
Combined molecular weight, pI and species searches select 13163 entries.
Pre searches select 13163 entries.

Data Set 1 Results

MS-Fit search selects 126 entries (results displayed for top 5 matches).

Results Summary

MOWSE
Score		#/60(%)
Masses
Matched 		%
Cov		%
TIC		Mean
Err
ppm		Data
Tol
ppm		MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
141456 (10)16.010.0-13.748.1 60716/8.3HUMANHypothetical protein KIAA0341
27144 (6)6.06.7-18.722.7 96957/7.3HUMANOxysterol binding protein-related protein 8 (OSBP-related protein 8) (ORP-8)
34304 (6)14.06.73.8183.8 50181/4.6HUMANFibulin-5 precursor (FIBL-5) (Developmental arteries and neural crest EGF-like protein) (Dance) (Urine p50 protein) (UP50)
44104 (6)14.06.7-13.782.9 49482/10.0MOUSEAlpha-(1,3)-fucosyltransferase (Galactoside 3-L-fucosyltransferase) (Fucosyltransferase 4) (FUCT-IV)
53936 (10)13.010.0-20.374.0 73244/6.7HUMANDEAD-box protein 3 (Helicase-like protein 2) (HLP2) (DEAD-box, X isoform)
99974.x.........................x....x..........xx......x........
80942...............................x..................x.x.x.....
96665................................x..........xx..x............
78578............................x..............x.x...x..........
3614................x.........x.............x.x..x...........x..


Detailed Results

1. 6/60 matches (10%).
Acc. #: Species: HUMAN Name: Hypothetical protein KIAA0341
Index: MW: 60716 Da pI: 8.3
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
864.4157864.4328-204884931(R) (T)
1307.59621307.6596-48961071(R) (G)
1475.67321475.6807-5.180910(R) (E)
1962.90051962.9045-2.02742910(R) (Q)
2011.93632012.0136-381471650(R) (A)
2247.11532247.0642232742931(R) (L)
2247.11532247.1266-5.01230(-) (Q)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 16% (92/546AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 4/60 matches (6%).
Acc. #: Species: HUMAN Name: Oxysterol binding protein-related protein 8 (OSBP-related protein 8) (ORP-8)
Index: MW: 96957 Da pI: 7.3
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1434.72691434.7745-334404510(K) (C)
2233.05502233.0678-5.76086251(K) (L)
2283.13582283.1729-165355550(K) (T)
2299.12312299.1678-191Met-ox 5355550(K)GILYGTMTLELGGTVNITCQK(T)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 6% (51/847AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 4/60 matches (6%).
Acc. #: Species: HUMAN Name: Fibulin-5 precursor (FIBL-5) (Developmental arteries and neural crest EGF-like protein) (Dance) (Urine p50 protein) (UP50)
Index: MW: 50181 Da pI: 4.6
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1475.67321475.6007492852950(R) (N)
1962.90051962.9714-363743901(R) (E)
2011.93632011.9911-273433591(R) (S)
2211.07282211.0075302963130(R) (C)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 14% (63/448AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 4/60 matches (6%).
Acc. #: Species: MOUSE Name: Alpha-(1,3)-fucosyltransferase (Galactoside 3-L-fucosyltransferase) (Fucosyltransferase 4) (FUCT-IV)
Index: MW: 49482 Da pI: 10.0
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1314.70071314.6483402152240(K) (T)
1962.90051962.9939-482762911(R) (T)
2082.94602082.9496-1.73944090(R) (T)
2229.07722229.1780-453323521(R) (F)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 14% (63/433AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 6/60 matches (10%).
Acc. #: Species: HUMAN Name: DEAD-box protein 3 (Helicase-like protein 2) (HLP2) (DEAD-box, X isoform)
Index: MW: 73244 Da pI: 6.7
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1051.61341051.5610503343421(R) (Y)
1183.46721183.5231-4756661(K) (D)
1838.88461838.9513-365325481(R) (N)
1940.89711940.9792-423363511(K) (M)
2082.94602083.0249-385125280(K) (I)
2662.40702662.4278-7.82312551(K) (E)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 13% (88/662AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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