MS-Fit Search Results

Parameters

Database searched: SwissProt.01.22.2003
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): 40-37
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 121920
Molecular weight search (1000 - 100000 Da) selects 115277 entries.
Full pI range: 121920 entries.
Species search ( HUMAN MOUSE ) selects 14893 entries.
Combined molecular weight, pI and species searches select 13163 entries.
Pre searches select 13163 entries.

Data Set 1 Results

MS-Fit search selects 470 entries (results displayed for top 5 matches).

Results Summary

	MOWSE Score	#/60(%) Masses Matched	% Cov	% TIC	Mean Err ppm	Data Tol ppm	Protein MW (Da)/pI	Species	Protein Name
1	2.676e+014	24 (40)	52.0	40.0	13.1	31.6	57914/8.0	HUMAN	Pyruvate kinase, M2 isozyme
2	2.675e+014	25 (41)	53.0	41.7	12.9	31.0	57937/8.0	HUMAN	Pyruvate kinase, M1 isozyme (Pyruvate kinase muscle isozyme) (Cytosolic thyroid hormone-binding protein) (CTHBP) (THBP1)
3	2.749e+010	19 (31)	35.0	31.7	10.4	33.5	57888/7.2	MOUSE	Pyruvate kinase, M2 isozyme
4	8238	9 (15)	14.0	15.0	7.70	44.1	92962/9.2	HUMAN	P300/CBP-associated factor (P/CAF) (Histone acetylase PCAF)
5	6395	5 (8)	20.0	8.3	-4.76	26.3	32789/7.1	HUMAN	Exosome complex exonuclease RRP4 (Ribosomal RNA processing protein 4)

9907	.	x	x	x	x	.	.	.	.	.	x	x	.	.	x	.	.	.	.	.	.	.	x	.	.	x	.	.	.	x	.	.	x	.	x	x	.	x	.	.	.	x	x	.	.	.	.	x	x	.	x	.	.	x	x	x	x	.	.	x
48470	x	x	x	x	x	.	.	.	.	.	x	x	.	.	x	.	.	.	.	.	.	.	x	.	.	x	.	.	.	x	.	.	x	.	x	x	.	x	.	.	.	x	x	.	.	.	.	x	x	.	x	.	.	x	x	x	x	.	.	x
72997	x	x	x	x	.	.	.	.	.	.	x	x	.	.	x	.	.	.	.	.	.	.	.	.	.	x	.	.	.	x	.	.	x	.	x	x	.	x	.	.	.	.	x	.	.	.	.	x	.	.	x	.	.	x	x	x	.	.	.	.
103340	x	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	x	.	.	x	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	x	.	x	.	.	x	.	.	.	.	.	.
94236	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	x	.	.	.	.	x	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.

Detailed Results

1. 24/60 matches (40%).
Acc. #: Species: HUMAN Name: Pyruvate kinase, M2 isozyme
Index: MW: 57914 Da pI: 8.0

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
953.4798	953.4805	-0.66		271	278	0	(K) (R)
1019.5203	1019.5162	4.0		368	376	0	(K) (M)
1109.5434	1109.5816	-34		271	279	1	(K) (F)
1197.6597	1197.6479	9.9		33	43	0	(R) (N)
1373.7393	1373.7211	13		44	56	0	(R) (S)
1462.8318	1462.8157	11		174	186	0	(K) (Q)
1482.7170	1482.6939	16		152	162	0	(K) (N)
1642.8020	1642.7713	19		476	489	0	(K) (V)
1779.9499	1779.8765	41		189	206	0	(K) (K)
1821.9120	1821.9169	-2.7		279	294	1	(R) (G)
1837.9534	1837.9118	23	1Met-ox	279	294	1	(R)RFDEILEASDGIMVAR(G)
1859.9227	1859.9002	12		231	246	0	(K) (K)
1875.9373	1875.8951	22	1Met-ox	231	246	0	(K)FGVEQDVDMVFASFIR(K)
1883.9311	1883.9040	14		74	89	0	(R) (N)
1923.0162	1922.9754	21	2Met-ox	320	336	0	(R)AGKPVICATQMLESMIK(K)
1932.0210	1931.9867	18		384	399	0	(R) (R)
2088.1190	2088.0878	15		384	400	1	(R) (L)
2175.1821	2175.1185	29		401	422	0	(R) (C)
2279.2277	2279.1607	29	1Met-ox	506	526	0	(K)GDVVIVLTGWRPGSGFTNTMR(V)
2349.2660	2349.2666	-0.22		208	230	1	(K) (F)
2391.2242	2391.2607	-15		505	526	1	(K) (V)
2407.3141	2407.2556	24	1Met-ox	505	526	1	(K)KGDVVIVLTGWRPGSGFTNTMR(V)
2465.3493	2465.2928	23		93	115	0	(R) (G)
2571.3523	2571.2917	24		468	489	1	(R) (V)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 52% (277/531AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 25/60 matches (41%).
Acc. #: Species: HUMAN Name: Pyruvate kinase, M1 isozyme (Pyruvate kinase muscle isozyme) (Cytosolic thyroid hormone-binding protein) (CTHBP) (THBP1)
Index: MW: 57937 Da pI: 8.0

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
884.4851 884.4776 8.4 1Met-ox 377 383 0 (R)MQHLIAR(E)
953.4798 953.4805 -0.66 271 278 0 (K) (R)
1019.5203 1019.5162 4.0 368 376 0 (K) (M)
1109.5434 1109.5816 -34 271 279 1 (K) (F)
1197.6597 1197.6479 9.9 33 43 0 (R) (N)
1373.7393 1373.7211 13 44 56 0 (R) (S)
1462.8318 1462.8157 11 174 186 0 (K) (Q)
1482.7170 1482.6939 16 152 162 0 (K) (N)
1642.8020 1642.7713 19 476 489 0 (K) (V)
1779.9499 1779.8765 41 189 206 0 (K) (K)
1821.9120 1821.9169 -2.7 279 294 1 (R) (G)
1837.9534 1837.9118 23 1Met-ox 279 294 1 (R)RFDEILEASDGIMVAR(G)
1859.9227 1859.9002 12 231 246 0 (K) (K)
1875.9373 1875.8951 22 1Met-ox 231 246 0 (K)FGVEQDVDMVFASFIR(K)
1883.9311 1883.9040 14 74 89 0 (R) (N)
1923.0162 1922.9754 21 2Met-ox 320 336 0 (R)AGKPVICATQMLESMIK(K)
1932.0210 1931.9867 18 384 399 0 (R) (R)
2088.1190 2088.0878 15 384 400 1 (R) (L)
2175.1821 2175.1185 29 401 422 0 (R) (C)
2279.2277 2279.1607 29 1Met-ox 506 526 0 (K)GDVVIVLTGWRPGSGFTNTMR(V)
2349.2660 2349.2666 -0.22 208 230 1 (K) (F)
2391.2242 2391.2607 -15 505 526 1 (K) (V)
2407.3141 2407.2556 24 1Met-ox 505 526 1 (K)KGDVVIVLTGWRPGSGFTNTMR(V)
2465.3493 2465.2928 23 93 115 0 (R) (G)
2571.3523 2571.2917 24 468 489 1 (R) (V)

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
884.4851	884.4776	8.4	1Met-ox	377	383	0	(R)MQHLIAR(E)
953.4798	953.4805	-0.66		271	278	0	(K) (R)
1019.5203	1019.5162	4.0		368	376	0	(K) (M)
1109.5434	1109.5816	-34		271	279	1	(K) (F)
1197.6597	1197.6479	9.9		33	43	0	(R) (N)
1373.7393	1373.7211	13		44	56	0	(R) (S)
1462.8318	1462.8157	11		174	186	0	(K) (Q)
1482.7170	1482.6939	16		152	162	0	(K) (N)
1642.8020	1642.7713	19		476	489	0	(K) (V)
1779.9499	1779.8765	41		189	206	0	(K) (K)
1821.9120	1821.9169	-2.7		279	294	1	(R) (G)
1837.9534	1837.9118	23	1Met-ox	279	294	1	(R)RFDEILEASDGIMVAR(G)
1859.9227	1859.9002	12		231	246	0	(K) (K)
1875.9373	1875.8951	22	1Met-ox	231	246	0	(K)FGVEQDVDMVFASFIR(K)
1883.9311	1883.9040	14		74	89	0	(R) (N)
1923.0162	1922.9754	21	2Met-ox	320	336	0	(R)AGKPVICATQMLESMIK(K)
1932.0210	1931.9867	18		384	399	0	(R) (R)
2088.1190	2088.0878	15		384	400	1	(R) (L)
2175.1821	2175.1185	29		401	422	0	(R) (C)
2279.2277	2279.1607	29	1Met-ox	506	526	0	(K)GDVVIVLTGWRPGSGFTNTMR(V)
2349.2660	2349.2666	-0.22		208	230	1	(K) (F)
2391.2242	2391.2607	-15		505	526	1	(K) (V)
2407.3141	2407.2556	24	1Met-ox	505	526	1	(K)KGDVVIVLTGWRPGSGFTNTMR(V)
2465.3493	2465.2928	23		93	115	0	(R) (G)
2571.3523	2571.2917	24		468	489	1	(R) (V)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 53% (284/531AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 19/60 matches (31%).
Acc. #: Species: MOUSE Name: Pyruvate kinase, M2 isozyme
Index: MW: 57888 Da pI: 7.2

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
884.4851 884.4776 8.4 1Met-ox 377 383 0 (R)MQHLIAR(E)
953.4798 953.4805 -0.66 271 278 0 (K) (R)
1019.5203 1019.5162 4.0 368 376 0 (K) (M)
1109.5434 1109.5816 -34 271 279 1 (K) (F)
1373.7393 1373.7211 13 44 56 0 (R) (S)
1462.8318 1462.8157 11 174 186 0 (K) (E)
1482.7170 1482.6939 16 152 162 0 (K) (N)
1779.9499 1779.8765 41 189 206 0 (K) (K)
1821.9120 1821.9169 -2.7 279 294 1 (R) (G)
1837.9534 1837.9118 23 1Met-ox 279 294 1 (R)RFDEILEASDGIMVAR(G)
1859.9227 1859.9002 12 231 246 0 (K) (K)
1875.9373 1875.8951 22 1Met-ox 231 246 0 (K)FGVEQDVDMVFASFIR(K)
1883.9311 1883.9040 14 74 89 0 (R) (N)
1932.0210 1931.9867 18 384 399 0 (R) (R)
2088.1190 2088.0878 15 384 400 1 (R) (L)
2279.2277 2279.1607 29 1Met-ox 506 526 0 (K)GDVVIVLTGWRPGSGFTNTMR(V)
2349.2660 2349.2666 -0.22 208 230 1 (K) (F)
2391.2242 2391.2607 -15 505 526 1 (K) (V)
2407.3141 2407.2556 24 1Met-ox 505 526 1 (K)KGDVVIVLTGWRPGSGFTNTMR(V)

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
884.4851	884.4776	8.4	1Met-ox	377	383	0	(R)MQHLIAR(E)
953.4798	953.4805	-0.66		271	278	0	(K) (R)
1019.5203	1019.5162	4.0		368	376	0	(K) (M)
1109.5434	1109.5816	-34		271	279	1	(K) (F)
1373.7393	1373.7211	13		44	56	0	(R) (S)
1462.8318	1462.8157	11		174	186	0	(K) (E)
1482.7170	1482.6939	16		152	162	0	(K) (N)
1779.9499	1779.8765	41		189	206	0	(K) (K)
1821.9120	1821.9169	-2.7		279	294	1	(R) (G)
1837.9534	1837.9118	23	1Met-ox	279	294	1	(R)RFDEILEASDGIMVAR(G)
1859.9227	1859.9002	12		231	246	0	(K) (K)
1875.9373	1875.8951	22	1Met-ox	231	246	0	(K)FGVEQDVDMVFASFIR(K)
1883.9311	1883.9040	14		74	89	0	(R) (N)
1932.0210	1931.9867	18		384	399	0	(R) (R)
2088.1190	2088.0878	15		384	400	1	(R) (L)
2279.2277	2279.1607	29	1Met-ox	506	526	0	(K)GDVVIVLTGWRPGSGFTNTMR(V)
2349.2660	2349.2666	-0.22		208	230	1	(K) (F)
2391.2242	2391.2607	-15		505	526	1	(K) (V)
2407.3141	2407.2556	24	1Met-ox	505	526	1	(K)KGDVVIVLTGWRPGSGFTNTMR(V)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 35% (189/531AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 9/60 matches (15%).
Acc. #: Species: HUMAN Name: P300/CBP-associated factor (P/CAF) (Histone acetylase PCAF)
Index: MW: 92962 Da pI: 9.2

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
884.4851 884.5093 -27 pyroGlu 242 249 0 (R)QTIVELAK(M)
1263.6940 1263.6632 24 551 561 1 (K) (M)
1543.7572 1543.7368 13 741 753 0 (K) (R)
1559.7465 1559.7317 9.5 1Met-ox 741 753 0 (K)SHQSAWPFMEPVK(R)
1656.8449 1656.8379 4.2 1Met-ox 431 444 1 (K)MTDSHVLEEAKKPR(V)
1941.0026 1940.9805 11 584 599 1 (K) (H)
2175.1821 2175.0922 41 250 266 1 (K) (R)
2279.2277 2279.2851 -25 510 528 1 (K) (M)
2349.2660 2349.2243 18 213 232 0 (K) (F)

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
884.4851	884.5093	-27	pyroGlu	242	249	0	(R)QTIVELAK(M)
1263.6940	1263.6632	24		551	561	1	(K) (M)
1543.7572	1543.7368	13		741	753	0	(K) (R)
1559.7465	1559.7317	9.5	1Met-ox	741	753	0	(K)SHQSAWPFMEPVK(R)
1656.8449	1656.8379	4.2	1Met-ox	431	444	1	(K)MTDSHVLEEAKKPR(V)
1941.0026	1940.9805	11		584	599	1	(K) (H)
2175.1821	2175.0922	41		250	266	1	(K) (R)
2279.2277	2279.2851	-25		510	528	1	(K) (M)
2349.2660	2349.2243	18		213	232	0	(K) (F)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 14% (118/832AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 5/60 matches (8%).
Acc. #: Species: HUMAN Name: Exosome complex exonuclease RRP4 (Ribosomal RNA processing protein 4)
Index: MW: 32789 Da pI: 7.1

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1019.5203 1019.5274 -7.0 96 103 1 (R) (L)
1859.9227 1859.9326 -5.3 25 41 0 (K) (G)
1875.9373 1875.9275 5.2 1Met-ox 25 41 0 (K)HLVVPGDTITTDTGFMR(G)
1913.9890 1914.0370 -25 104 121 0 (R) (R)
1946.0141 1945.9979 8.4 270 285 0 (K) (Q)

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
1019.5203	1019.5274	-7.0		96	103	1	(R) (L)
1859.9227	1859.9326	-5.3		25	41	0	(K) (G)
1875.9373	1875.9275	5.2	1Met-ox	25	41	0	(K)HLVVPGDTITTDTGFMR(G)
1913.9890	1914.0370	-25		104	121	0	(R) (R)
1946.0141	1945.9979	8.4		270	285	0	(K) (Q)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 20% (59/293AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):