MS-Fit Search Results



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Parameters

Database searched: SwissProt.01.22.2003
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): 40-40
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 121920
Molecular weight search (1000 - 100000 Da) selects 115277 entries.
Full pI range: 121920 entries.
Species search ( HUMAN MOUSE ) selects 14893 entries.
Combined molecular weight, pI and species searches select 13163 entries.
Pre searches select 13163 entries.

Data Set 1 Results

MS-Fit search selects 517 entries (results displayed for top 5 matches).

Results Summary

MOWSE
Score
#/59(%)
Masses
Matched
%
Cov
%
TIC
Mean
Err
ppm
Data
Tol
ppm
MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
14.231e+00921 (35)44.035.6-6.8721.0 59751/9.2HUMANATP synthase alpha chain, mitochondrial precursor
21.916e+00819 (32)39.032.2-9.7625.5 59753/9.2MOUSEATP synthase alpha chain, mitochondrial precursor
37.789e+0048 (13)23.013.6-8.8622.3 55993/8.8HUMANSerine hydroxymethyltransferase, mitochondrial precursor (Serine methylase) (Glycine hydroxymethyltransferase) (SHMT)
42.542e+0048 (13)17.013.6-4.5081.4 77888/8.7HUMANZinc finger protein 7 (Zinc finger protein KOX4) (Zinc finger protein HF.16)
566827 (11)9.011.9-8.4055.8 93393/9.3MOUSEGeneral control of amino acid synthesis protein 5-like 2 (Histone acetyltransferase GCN5) (mmGCN5)
76648xxxx...xx.x.x.x...xxx.x.x...x..x........x.......x..xx..x...
30304xxxx...xx.x.x.x...x.x.x.x...x..x........x.......x..xx......
9112......x.x.......x.x.......x.x........x.....x...............
49976...............x.x..............xx.....x.xx....x...........
51319........................x.x....x..x.....x............xx....



Detailed Results

1. 21/59 matches (35%).
Acc. #: Species: HUMAN Name: ATP synthase alpha chain, mitochondrial precursor
Index: MW: 59751 Da pI: 9.2
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
876.4747876.4977-261Met-ox 3233290(R)QMSLLLR(R)
892.4809892.4892-9.34354410(K) (E)
1000.56101000.5791-1874830(R) (V)
1026.58221026.5948-121952040(K) (G)
1287.67431287.6949-163063160(K) (Q)
1316.72051316.7426-172192300(K) (R)
1341.71431341.7241-7.32422520(K) (R)
1438.83971438.8494-6.84034160(K) (V)
1497.81461497.8252-7.12422531(K) (S)
1548.82621548.8684-273173291(K) (R)
1551.84061551.8423-1.15405531(K) (-)
1553.73711553.7389-1.13353470(R) (L)
1575.77951575.7866-4.559730(R) (V)
1624.88571624.8910-3.21341490(R) (V)
1683.79141683.79000.841Met-ox 891030(R)NVQAEEMVEFSSGLK(G)
1780.96211780.9921-171331491(K) (V)
2120.04422120.03345.11Met-ox 1041230(K)GMSLNLEPDNVGVVVFGNDK(L)
2308.13652308.12843.5pyroGlu 1Met-ox 4734930(K)QGQYSPMAIEEQVAVIYAGVR(G)
2325.17642325.15499.21Met-ox 4734930(K)QGQYSPMAIEEQVAVIYAGVR(G)
2338.17252338.16792.04424630(R) (G)
2367.27812367.25738.85075270(K) (A)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 44% (247/553AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 19/59 matches (32%).
Acc. #: Species: MOUSE Name: ATP synthase alpha chain, mitochondrial precursor
Index: MW: 59753 Da pI: 9.2
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
876.4747876.4977-261Met-ox 3233290(R)QMSLLLR(R)
892.4809892.4892-9.34354410(K) (E)
1000.56101000.5791-1874830(R) (V)
1000.56101000.6043-431241321(K) (R)
1026.58221026.5948-121952040(K) (G)
1287.67431287.6949-163063160(K) (Q)
1316.72051316.7426-172192300(K) (R)
1341.71431341.7241-7.32422520(K) (R)
1438.83971438.8494-6.84034160(K) (V)
1497.81461497.8252-7.12422531(K) (S)
1548.82621548.8684-273173291(K) (R)
1553.73711553.7389-1.13353470(R) (L)
1575.77951575.7866-4.559730(R) (V)
1624.88571624.8910-3.21341490(R) (V)
1683.79141683.79000.841Met-ox 891030(R)NVQAEEMVEFSSGLK(G)
1780.96211780.9921-171331491(K) (V)
2120.04422120.03345.11Met-ox 1041230(K)GMSLNLEPDNVGVVVFGNDK(L)
2308.13652308.12843.5pyroGlu 1Met-ox 4734930(K)QGQYSPMAIEEQVAVIYAGVR(G)
2325.17642325.15499.21Met-ox 4734930(K)QGQYSPMAIEEQVAVIYAGVR(G)
2338.17252338.16792.04424630(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 39% (221/553AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 8/59 matches (13%).
Acc. #: Species: HUMAN Name: Serine hydroxymethyltransferase, mitochondrial precursor (Serine methylase) (Glycine hydroxymethyltransferase) (SHMT)
Index: MW: 55993 Da pI: 8.8
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1169.51391169.5479-293063140(R) (I)
1316.72051316.7439-184264371(R) (E)
1509.72461509.7372-8.370820(R) (A)
1548.82621548.8386-8.02012140(K) (L)
1636.79671636.79332.11Met-ox 1831960(R)ISATSIFFESMPYK(L)
1683.79141683.77996.826410(R) (G)
2027.96402027.9748-5.41051210(R) (R)
2240.17862240.2039-113693890(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 23% (116/504AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 8/59 matches (13%).
Acc. #: Species: HUMAN Name: Zinc finger protein 7 (Zinc finger protein KOX4) (Zinc finger protein HF.16)
Index: MW: 77888 Da pI: 8.7
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1506.71901506.7930-494774890(K) (I)
1535.72221535.7640-271Met-ox 5335450(K)AFSMSTQLTIHQR(V)
1819.96691819.8835462712851(R) (A)
1820.92241820.9983-423894051(K) (I)
2082.99442083.0936-482072241(R) (C)
2211.11592211.0756181431610(K) (T)
2225.11132225.0304366066240(R) (K)
2284.13842284.0715294714891(K) (I)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 17% (120/686AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 7/59 matches (11%).
Acc. #: Species: MOUSE Name: General control of amino acid synthesis protein 5-like 2 (Histone acetyltransferase GCN5) (mmGCN5)
Index: MW: 93393 Da pI: 9.3
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1624.88571624.9063-134905040(R) (A)
1636.79671636.8382-251151281(K) (M)
1780.96211781.0074-254895041(R) (A)
1940.95771940.9805-125825971(K) (H)
2120.04422119.9516442903050(R) (Y)
2349.05672349.1486-39pyroGlu 2472641(R)QTMFELSKMFLLCLNYWK(L)
2366.20302366.1751122472641(R) (L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 9% (80/830AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):


MS-Fit in ProteinProspector 4.0.4
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