MS-Fit Search Results

MS-Fit Search Results

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Parameters

Database searched: SwissProt.01.22.2003
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): 40-43
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 121920
Molecular weight search (1000 - 100000 Da) selects 115277 entries.
Full pI range: 121920 entries.
Species search ( HUMAN MOUSE ) selects 14893 entries.
Combined molecular weight, pI and species searches select 13163 entries.
Pre searches select 13163 entries.

Data Set 1 Results

MS-Fit search selects 214 entries (results displayed for top 5 matches).

Results Summary

	MOWSE Score	#/60(%) Masses Matched	% Cov	% TIC	Mean Err ppm	Data Tol ppm	MS-Digest Index #	Protein MW (Da)/pI	Accession #	Species	Protein Name
1	1.158e+005	6 (10)	21.0	10.0	14.5	41.0		57222/9.2		HUMAN	Polypyrimidine tract-binding protein 1 (PTB) (Heterogeneous nuclear ribonucleoprotein I) (hnRNP I) (57 kDa RNA-binding protein PPTB-1)
2	2.192e+004	5 (8)	16.0	8.3	18.7	39.6		56479/8.5		MOUSE	Polypyrimidine tract-binding protein 1 (PTB) (Heterogeneous nuclear ribonucleoprotein I) (hnRNP I)
3	6137	5 (8)	8.0	8.3	15.1	47.4		60619/7.9		MOUSE	Neuronal proto-oncogene tyrosine-protein kinase SRC (p60-SRC) (C-SRC)
4	2134	6 (10)	14.0	10.0	14.1	55.7		70223/7.2		HUMAN	Hepatocellular carcinoma-associated antigen 66
5	1576	5 (8)	15.0	8.3	-9.09	67.3		44931/6.9		MOUSE	2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial precursor (AKB ligase) (Glycine acetyltransferase)

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33736	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	x	x	.	.	.	.	x	.	.	.	.	.	.	.
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Detailed Results

1. 6/60 matches (10%).
Acc. #: Species: HUMAN Name: Polypyrimidine tract-binding protein 1 (PTB) (Heterogeneous nuclear ribonucleoprotein I) (hnRNP I) (57 kDa RNA-binding protein PPTB-1)
Index: MW: 57222 Da pI: 9.2

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
1431.7446	1431.7385	4.2		123	134	0	(R) (E)
1925.9117	1925.9213	-5.0		239	254	0	(K) (I)
2039.1322	2039.0966	17		349	366	0	(R) (V)
2243.2148	2243.1209	42		219	238	0	(K) (L)
2275.3566	2275.2774	35		326	348	0	(R) (V)
2705.3075	2705.3252	-6.5		374	398	1	(K) (L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 21% (114/531AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 5/60 matches (8%).
Acc. #: Species: MOUSE Name: Polypyrimidine tract-binding protein 1 (PTB) (Heterogeneous nuclear ribonucleoprotein I) (hnRNP I)
Index: MW: 56479 Da pI: 8.5

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1431.7446 1431.7385 4.2 122 133 0 (R) (E)
1925.9117 1925.9213 -5.0 238 253 0 (K) (I)
2039.1322 2039.0966 17 347 364 0 (R) (V)
2243.2148 2243.1209 42 218 237 0 (K) (L)
2275.3566 2275.2774 35 324 346 0 (R) (V)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 16% (89/527AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 5/60 matches (8%).
Acc. #: Species: MOUSE Name: Neuronal proto-oncogene tyrosine-protein kinase SRC (p60-SRC) (C-SRC)
Index: MW: 60619 Da pI: 7.9

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
886.4316 886.4423 -12 266 272 0 (K) (E)
2179.1375 2179.0891 22 368 387 0 (R) (M)
2195.1219 2195.0840 17 1Met-ox 368 387 0 (R)LPQLVDMSAQIASGMAYVER(M)
2211.1885 2211.0790 50 2Met-ox 368 387 0 (R)LPQLVDMSAQIASGMAYVER(M)
2299.2563 2299.2590 -1.2 447 466 1 (R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 8% (47/541AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 6/60 matches (10%).
Acc. #: Species: HUMAN Name: Hepatocellular carcinoma-associated antigen 66
Index: MW: 70223 Da pI: 7.2

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
939.4652 939.4974 -34 1Met-ox 513 519 1 (R)KMIQFEK(E)
1228.6234 1228.6115 9.7 478 486 1 (K) (S)
1365.6349 1365.5891 34 520 530 0 (K) (E)
2284.2484 2284.1865 27 52 69 0 (K) (R)
2299.2563 2299.2459 4.5 129 149 0 (K) (W)
2662.4908 2662.3737 44 366 386 1 (K) (E)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 14% (87/597AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 5/60 matches (8%).
Acc. #: Species: MOUSE Name: 2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial precursor (AKB ligase) (Glycine acetyltransferase)
Index: MW: 44931 Da pI: 6.9

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1179.5493 1179.5832 -29 401 410 0 (R) (L)
1651.0373 1650.9623 45 366 380 1 (K) (G)
1733.7451 1733.8045 -34 1 15 0 (-) (R)
1765.7420 1765.7943 -30 2Met-ox 1 15 0 (-)MWASFMWHGALSPGR(R)
2300.2404 2300.2363 1.8 263 285 1 (K) (S)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 15% (63/416AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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