MS-Fit Search Results

MS-Fit Search Results

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Parameters

Database searched: SwissProt.01.22.2003
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): 40-44
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 121920
Molecular weight search (1000 - 100000 Da) selects 115277 entries.
Full pI range: 121920 entries.
Species search ( HUMAN MOUSE ) selects 14893 entries.
Combined molecular weight, pI and species searches select 13163 entries.
Pre searches select 13163 entries.

Data Set 1 Results

MS-Fit search selects 856 entries (results displayed for top 5 matches).

Results Summary

	MOWSE Score	#/62(%) Masses Matched	% Cov	% TIC	Mean Err ppm	Data Tol ppm	MS-Digest Index #	Protein MW (Da)/pI	Accession #	Species	Protein Name
1	8.839e+005	11 (17)	27.0	17.7	25.8	36.1		51066/8.7		MOUSE	Eukaryotic translation initiation factor 2 subunit 3, X-linked (Eukaryotic translation initiation factor 2 gamma subunit, X-linked) (eIF-2-gamma X)
2	8.832e+005	12 (19)	31.0	19.4	27.2	35.7		51110/8.7		HUMAN	Eukaryotic translation initiation factor 2 subunit 3 (Eukaryotic translation initiation factor 2 gamma subunit) (eIF-2-gamma)
3	3.758e+004	10 (16)	26.0	16.1	25.4	37.3		51131/8.8		MOUSE	Eukaryotic translation initiation factor 2 subunit 3, Y-linked (Eukaryotic translation initiation factor 2 gamma subunit, Y-linked) (eIF-2-gamma Y)
4	9738	10 (16)	18.0	16.1	20.9	63.8		64763/6.0		MOUSE	Protein Tara (Trio-associated repeat on actin)
5	2464	5 (8)	17.0	8.1	36.5	68.2		36926/9.0		MOUSE	Beta-1,3-N-acetylglucosaminyltransferase radical fringe

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Detailed Results

1. 11/62 matches (17%).
Acc. #: Species: MOUSE Name: Eukaryotic translation initiation factor 2 subunit 3, X-linked (Eukaryotic translation initiation factor 2 gamma subunit, X-linked) (eIF-2-gamma X)
Index: MW: 51066 Da pI: 8.7

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
851.4024	851.3899	15		249	255	0	(R) (L)
888.4642	888.4613	3.3		343	349	0	(K) (A)
935.5151	935.4951	21		69	75	1	(R) (N)
939.5305	939.5376	-7.5		60	68	0	(K) (F)
942.5688	942.5525	17		453	460	0	(R) (R)
1251.7404	1251.7061	27		28	38	0	(K) (Q)
1510.9218	1510.8593	41		290	303	0	(K) (D)
1572.8911	1572.8307	38		427	440	0	(K) (I)
1662.9022	1662.8485	32	1Met-ox	401	416	0	(K)NEVLMVNIGSLSTGGR(V)
1692.8249	1692.7474	46		91	103	0	(K) (S)
2570.4903	2570.3619	50		318	342	0	(K) (I)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 27% (131/472AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 12/62 matches (19%).
Acc. #: Species: HUMAN Name: Eukaryotic translation initiation factor 2 subunit 3 (Eukaryotic translation initiation factor 2 gamma subunit) (eIF-2-gamma)
Index: MW: 51110 Da pI: 8.7

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
851.4024 851.3899 15 249 255 0 (R) (L)
888.4642 888.4613 3.3 343 349 0 (K) (A)
935.5151 935.4951 21 69 75 1 (R) (N)
939.5305 939.5376 -7.5 60 68 0 (K) (F)
942.5688 942.5525 17 453 460 0 (R) (R)
1251.7404 1251.7061 27 28 38 0 (K) (Q)
1510.9218 1510.8593 41 290 303 0 (K) (D)
1572.8911 1572.8307 38 427 440 0 (K) (I)
1591.8868 1591.8192 42 AcetN 1 17 0 (-)AGGEAGVTLGQPHLSR(Q)
1662.9022 1662.8485 32 1Met-ox 401 416 0 (K)NEVLMVNIGSLSTGGR(V)
1692.8249 1692.7474 46 91 103 0 (K) (S)
2570.4903 2570.3619 50 318 342 0 (K) (I)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 31% (148/472AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 10/62 matches (16%).
Acc. #: Species: MOUSE Name: Eukaryotic translation initiation factor 2 subunit 3, Y-linked (Eukaryotic translation initiation factor 2 gamma subunit, Y-linked) (eIF-2-gamma Y)
Index: MW: 51131 Da pI: 8.8

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
851.4024 851.3899 15 249 255 0 (R) (L)
888.4642 888.4613 3.3 343 349 0 (K) (A)
935.5151 935.4951 21 69 75 1 (R) (N)
939.5305 939.5376 -7.5 60 68 0 (K) (F)
942.5688 942.5525 17 453 460 0 (R) (R)
1510.9218 1510.8593 41 290 303 0 (K) (D)
1572.8911 1572.8307 38 427 440 0 (K) (I)
1591.8868 1591.8192 42 AcetN 1 17 0 (-)AGGEAGVTLGQPHLSR(Q)
1662.9022 1662.8485 32 1Met-ox 401 416 0 (K)NEVLMVNIGSLSTGGR(V)
2570.4903 2570.3619 50 318 342 0 (K) (I)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 26% (124/472AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 10/62 matches (16%).
Acc. #: Species: MOUSE Name: Protein Tara (Trio-associated repeat on actin)
Index: MW: 64763 Da pI: 6.0

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
939.5305 939.4648 70 283 291 0 (R) (L)
946.5589 946.5144 47 495 501 1 (K) (D)
972.5221 972.5366 -15 502 509 0 (R) (D)
974.5684 974.5345 35 544 552 0 (R) (E)
988.5140 988.5063 7.8 553 561 1 (K) (-)
990.5558 990.5294 27 1Met-ox 544 552 0 (R)LAMAALQEK(E)
1561.7817 1561.8451 -41 241 254 0 (R) (R)
1659.8608 1659.8488 7.2 410 423 1 (K) (E)
1662.9022 1662.8451 34 99 113 1 (K) (I)
1954.0793 1954.0074 37 513 528 1 (R) (T)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 18% (101/561AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 5/62 matches (8%).
Acc. #: Species: MOUSE Name: Beta-1,3-N-acetylglucosaminyltransferase radical fringe
Index: MW: 36926 Da pI: 9.0

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
871.4110 871.3871 27 128 134 0 (K) (F)
1179.5931 1179.5614 27 110 119 0 (R) (T)
1659.8608 1659.7545 64 122 134 1 (R) (F)
1941.0249 1941.0459 -11 253 268 0 (R) (L)
2211.2048 2211.0392 75 90 109 0 (R) (M)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 17% (59/332AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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