MS-Fit Search Results


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Parameters

Database searched: SwissProt.01.22.2003
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): 40-46
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 121920
Molecular weight search (10000 - 100000 Da) selects 104019 entries.
Full pI range: 121920 entries.
Species search ( HUMAN MOUSE ) selects 14893 entries.
Combined molecular weight, pI and species searches select 12734 entries.
Pre searches select 12734 entries.

Data Set 1 Results

MS-Fit search selects 240 entries (results displayed for top 5 matches).

Results Summary

MOWSE
Score		#/43(%)
Masses
Matched 		%
Cov		%
TIC		Mean
Err
ppm		Data
Tol
ppm		MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
13.331e+00412 (27)31.027.917.247.4 50141/9.1HUMANElongation factor 1-alpha 1 (EF-1-alpha-1) (Elongation factor 1 A-1) (eEF1A-1) (Elongation factor Tu) (EF-Tu)
23.329e+00412 (27)31.027.917.247.4 50164/9.1MOUSEElongation factor 1-alpha 1 (EF-1-alpha-1) (Elongation factor 1 A-1) (eEF1A-1) (Elongation factor Tu) (EF-Tu)
33.030e+0049 (20)18.020.910.346.4 50455/9.1MOUSEElongation factor 1-alpha 2 (EF-1-alpha-2) (Elongation factor 1 A-2) (eEF1A-2) (Statin S1)
43.029e+0049 (20)18.020.910.346.4 50471/9.1HUMANElongation factor 1-alpha 2 (EF-1-alpha-2) (Elongation factor 1 A-2) (eEF1A-2) (Statin S1)
52485 (11)9.011.639.242.1 76008/6.2MOUSECyclic-AMP-dependent transcription factor ATF-6 beta (Activating transcription factor 6 beta) (ATF6-beta) (cAMP responsive element binding protein-like 1) (cAMP response element binding protein-related protein) (Creb-rp)
3482.x.xxx..x..x...x..x......x.......x....x...x
104637.x.xxx..x..x...x..x......x.......x....x...x
29795.x.xx...x..x...x..x.......x......x.........
101180.x.xx...x..x...x..x.......x......x.........
117048........x............x....x....x..x........


Detailed Results

1. 12/43 matches (27%).
Acc. #: Species: HUMAN Name: Elongation factor 1-alpha 1 (EF-1-alpha-1) (Elongation factor 1 A-1) (eEF1A-1) (Elongation factor Tu) (EF-Tu)
Index: MW: 50141 Da pI: 9.1
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
870.5250870.5413-191471540(K) (M)
897.5242897.5409-19pyroGlu 4314390(R)QTVAVGVIK(A)
914.5530914.5675-164314390(R) (A)
936.5437936.5155301661721(K) (E)
975.5578975.55156.52482550(R) (I)
1025.63511025.6108242562660(K) (V)
1120.61971120.60021721300(K) (C)
1314.77221314.7422231351460(R) (Q)
1404.77791404.72763685960(K) (D)
1588.94701588.8811416200(K) (S)
2531.49772531.3795471Met-ox 2672900(R)VETGVLKPGMVVTFAPVNVTTEVK(S)
3041.51423041.4059362Met-ox 3964230(K)SGDAAIVDMVPGKPMCVESFSDYPPLGR(F)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 31% (144/462AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 12/43 matches (27%).
Acc. #: Species: MOUSE Name: Elongation factor 1-alpha 1 (EF-1-alpha-1) (Elongation factor 1 A-1) (eEF1A-1) (Elongation factor Tu) (EF-Tu)
Index: MW: 50164 Da pI: 9.1
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
870.5250870.5413-191471540(K) (M)
897.5242897.5409-19pyroGlu 4314390(R)QTVAVGVIK(A)
914.5530914.5675-164314390(R) (A)
936.5437936.5155301661721(K) (E)
975.5578975.55156.52482550(R) (I)
1025.63511025.6108242562660(K) (V)
1120.61971120.60021721300(K) (C)
1314.77221314.7422231351460(R) (Q)
1404.77791404.72763685960(K) (D)
1588.94701588.8811416200(K) (S)
2531.49772531.3795471Met-ox 2672900(R)VETGVLKPGMVVTFAPVNVTTEVK(S)
3041.51423041.4059362Met-ox 3964230(K)SGDAAIVDMVPGKPMCVESFSDYPPLGR(F)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 31% (144/462AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 9/43 matches (20%).
Acc. #: Species: MOUSE Name: Elongation factor 1-alpha 2 (EF-1-alpha-2) (Elongation factor 1 A-2) (eEF1A-2) (Statin S1)
Index: MW: 50455 Da pI: 9.1
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
870.5250870.5413-191471540(K) (M)
897.5242897.5409-19pyroGlu 4314390(R)QTVAVGVIK(N)
914.5530914.5675-164314390(R) (N)
975.5578975.55156.52482550(R) (I)
1025.63511025.6108242562660(K) (V)
1120.61971120.60021721300(K) (C)
1314.77221314.7422231351460(R) (Q)
1418.79131418.74323485960(K) (D)
1588.94701588.8811416200(K) (S)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 18% (85/463AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 9/43 matches (20%).
Acc. #: Species: HUMAN Name: Elongation factor 1-alpha 2 (EF-1-alpha-2) (Elongation factor 1 A-2) (eEF1A-2) (Statin S1)
Index: MW: 50471 Da pI: 9.1
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
870.5250870.5413-191471540(K) (M)
897.5242897.5409-19pyroGlu 4314390(R)QTVAVGVIK(N)
914.5530914.5675-164314390(R) (N)
975.5578975.55156.52482550(R) (I)
1025.63511025.6108242562660(K) (V)
1120.61971120.60021721300(K) (C)
1314.77221314.7422231351460(R) (Q)
1418.79131418.74323485960(K) (D)
1588.94701588.8811416200(K) (S)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 18% (85/463AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 5/43 matches (11%).
Acc. #: Species: MOUSE Name: Cyclic-AMP-dependent transcription factor ATF-6 beta (Activating transcription factor 6 beta) (ATF6-beta) (cAMP responsive element binding protein-like 1) (cAMP response element binding protein-related protein) (Creb-rp)
Index: MW: 76008 Da pI: 6.2
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
975.5578975.5264324734820(R) (E)
1357.76331357.75923.05976080(R) (T)
1418.79131418.7068605865961(R) (D)
1418.79131418.7068605855951(R) (R)
1482.74401482.6899374884990(R) (H)
1780.05461779.9757445435590(K) (D)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 9% (63/699AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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