MS-Fit Search Results

MS-Fit Search Results

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Parameters

Database searched: SwissProt.01.22.2003
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): 40-48
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 121920
Molecular weight search (1000 - 100000 Da) selects 115277 entries.
Full pI range: 121920 entries.
Species search ( HUMAN MOUSE ) selects 14893 entries.
Combined molecular weight, pI and species searches select 13163 entries.
Pre searches select 13163 entries.

Data Set 1 Results

MS-Fit search selects 385 entries (results displayed for top 5 matches).

Results Summary

	MOWSE Score	#/60(%) Masses Matched	% Cov	% TIC	Mean Err ppm	Data Tol ppm	MS-Digest Index #	Protein MW (Da)/pI	Accession #	Species	Protein Name
1	7.042e+009	20 (33)	49.0	33.3	11.3	21.4		59266/6.4		HUMAN	Glucose-6-phosphate 1-dehydrogenase (G6PD)
2	7.738e+008	17 (28)	41.0	28.3	5.60	27.1		56651/6.3		HUMAN	D-3-phosphoglycerate dehydrogenase (3-PGDH)
3	1.161e+004	11 (18)	22.0	18.3	14.9	23.1		59263/6.1		MOUSE	Glucose-6-phosphate 1-dehydrogenase X (G6PD)
4	6476	8 (13)	14.0	13.3	6.65	45.3		59556/5.4		MOUSE	T-COMPLEX PROTEIN 1, THETA SUBUNIT (TCP-1-THETA) (CCT-THETA)
5	3425	6 (10)	15.0	10.0	11.8	17.8		51449/6.5		MOUSE	D-3-phosphoglycerate dehydrogenase (3-PGDH) (A10)

84510	x	x	.	.	.	x	x	.	x	x	x	x	x	x	.	.	.	x	.	.	.	.	.	.	.	x	.	.	.	x	.	.	.	.	.	x	.	x	.	x	.	x	.	.	x	.	.	.	.	.	.	.	x	.	.	.	.	.	.	x
39398	x	.	x	.	.	.	.	x	.	.	.	.	.	.	x	.	x	.	x	x	.	x	x	x	x	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	x	.	.	x	.	.	.	x	.	.	.	.	.	x	x	.	.
76577	x	x	.	.	.	x	x	.	x	.	.	.	x	x	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	x	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.
90887	.	.	.	.	.	x	.	.	.	x	.	x	.	x	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	x	.	x	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
91099	x	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	x	.	.	.	.	.	.	.	.	.	.	x	.	.

Detailed Results

1. 20/60 matches (33%).
Acc. #: Species: HUMAN Name: Glucose-6-phosphate 1-dehydrogenase (G6PD)
Index: MW: 59266 Da pI: 6.4

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
899.5803	899.5427	42		387	393	1	(K) (V)
915.5080	915.4900	20	AcetN	1	9	0	(-)AEQVALSR(T)
915.5080	915.5151	-7.7		90	97	1	(K) (L)
1002.5683	1002.5525	16		220	227	0	(R) (D)
1058.6466	1058.6362	9.7		167	175	0	(R) (D)
1102.6197	1102.6049	13		349	357	0	(R) (C)
1132.5849	1132.5639	19		321	330	0	(K) (G)
1138.6409	1138.6261	13		96	104	1	(K) (N)
1173.6556	1173.6380	15		183	192	0	(R) (E)
1273.6406	1273.6217	15		247	257	0	(R) (D)
1307.7012	1307.7033	-1.6		228	238	0	(R) (E)
1394.7736	1394.7625	8.0		48	57	0	(K) (D)
1664.8828	1664.8648	11		58	72	0	(R) (S)
1761.8001	1761.7801	11	1Met-ox	153	166	0	(K)NIHESCMSQIGWNR(I)
1807.7961	1807.7887	4.1		105	120	0	(R) (L)
1851.9331	1851.9250	4.4	1Met-ox	440	454	0	(R)LILDVFCGSQMHFVR(S)
1927.9829	1927.9765	3.3		331	348	0	(R) (W)
1956.9599	1956.9390	11		371	386	0	(R) (R)
2181.1577	2181.1014	26		228	246	1	(R) (G)
2360.1158	2360.0869	12	1Met-ox	408	427	0	(K)KPGMFFNPEESELDLTYGNR(Y)
2852.5944	2852.6078	-4.7		464	487	0	(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 49% (255/515AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 17/60 matches (28%).
Acc. #: Species: HUMAN Name: D-3-phosphoglycerate dehydrogenase (3-PGDH)
Index: MW: 56651 Da pI: 6.3

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
899.5803 899.5678 14 147 155 0 (K) (I)
986.6160 986.6039 12 462 469 0 (R) (T)
1099.6290 1099.6111 16 237 247 0 (R) (A)
1345.7606 1345.7691 -6.3 352 364 0 (K) (N)
1390.6678 1390.6544 9.6 523 533 0 (K) (-)
1390.6678 1390.7000 -23 1Met-ox 120 132 1 (R)QIPQATASMKDGK(W)
1426.7855 1426.8270 -29 21 33 1 (R) (Q)
1462.7190 1462.7034 11 9 20 0 (K) (K)
1488.7536 1488.7294 16 76 90 0 (R) (K)
1590.8235 1590.7984 16 8 20 1 (R) (K)
1590.8235 1590.7984 16 9 21 1 (K) (I)
1639.9281 1639.9205 4.6 365 380 0 (K) (E)
1654.8056 1654.7821 14 1Met-ox 295 308 0 (R)CGEEIAVQFVDMVK(G)
1900.9746 1900.9690 2.9 39 54 0 (K) (S)
2062.0387 2062.0391 -0.20 271 289 0 (R) (E)
2231.1297 2231.0767 24 248 268 0 (R) (D)
2304.1795 2304.1579 9.4 2Met-ox 470 491 0 (R)TQTSDPAMLPTMIGLLAEAGVR(L)
2471.2738 2471.2816 -3.2 34 54 1 (K) (S)
2502.2118 2502.2047 2.8 248 270 1 (R) (A)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 41% (221/533AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 11/60 matches (18%).
Acc. #: Species: MOUSE Name: Glucose-6-phosphate 1-dehydrogenase X (G6PD)
Index: MW: 59263 Da pI: 6.1

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
899.5803 899.5427 42 387 393 1 (K) (V)
915.5080 915.4900 20 AcetN 1 9 0 (-)AEQVALSR(T)
1002.5683 1002.5525 16 220 227 0 (R) (D)
1058.6466 1058.6362 9.7 167 175 0 (R) (D)
1102.6197 1102.6049 13 349 357 0 (R) (C)
1273.6406 1273.6217 15 247 257 0 (R) (D)
1307.7012 1307.7033 -1.6 228 238 0 (R) (E)
1394.7736 1394.7625 8.0 48 57 0 (K) (D)
1851.9331 1851.9250 4.4 1Met-ox 440 454 0 (R)LILDVFCGSQMHFVR(S)
2181.1577 2181.1014 26 228 246 1 (R) (G)
2360.1158 2360.0869 12 1Met-ox 408 427 0 (K)KPGMFFNPEESELDLTYGNR(Y)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 22% (117/515AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 8/60 matches (13%).
Acc. #: Species: MOUSE Name: T-COMPLEX PROTEIN 1, THETA SUBUNIT (TCP-1-THETA) (CCT-THETA)
Index: MW: 59556 Da pI: 5.4

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1002.5683 1002.5770 -8.7 55 62 1 (K) (L)
1132.5849 1132.5850 -0.11 156 165 0 (R) (T)
1173.6556 1173.6479 6.6 368 378 0 (K) (G)
1307.7012 1307.6384 48 21 31 0 (K) (N)
1654.8056 1654.8297 -15 139 152 0 (K) (N)
1779.8807 1779.9162 -20 156 171 1 (R) (Q)
1795.9566 1795.9112 25 1Met-ox 156 171 1 (R)DVDEVSSLLRTSIMSK(Q)
1851.9331 1851.9023 17 38 54 1 (K) (M)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 14% (77/548AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 6/60 matches (10%).
Acc. #: Species: MOUSE Name: D-3-phosphoglycerate dehydrogenase (3-PGDH) (A10)
Index: MW: 51449 Da pI: 6.5

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
899.5803 899.5678 14 99 107 0 (K) (I)
1099.6290 1099.6111 16 189 199 0 (R) (A)
1654.8056 1654.7821 14 1Met-ox 247 260 0 (R)CGEEIAVQFVDMVK(G)
2062.0387 2062.0391 -0.20 223 241 0 (R) (E)
2231.1297 2231.0767 24 200 220 0 (R) (D)
2502.2118 2502.2047 2.8 200 222 1 (R) (A)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 15% (76/485AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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