MS-Fit Search Results

MS-Fit Search Results

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Parameters

Database searched: SwissProt.01.22.2003
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): 40-48
Minimum Matches: 3
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 121920
Molecular weight search (1000 - 100000 Da) selects 115277 entries.
Full pI range: 121920 entries.
Species search ( HUMAN MOUSE ) selects 14893 entries.
Combined molecular weight, pI and species searches select 13163 entries.
Pre searches select 13163 entries.

Data Set 1 Results

MS-Fit search selects 367 entries (results displayed for top 5 matches).

Results Summary

	MOWSE Score	#/40(%) Masses Matched	% Cov	% TIC	Mean Err ppm	Data Tol ppm	MS-Digest Index #	Protein MW (Da)/pI	Accession #	Species	Protein Name
1	2.700e+008	16 (40)	39.0	40.0	5.14	27.6		56651/6.3		HUMAN	D-3-phosphoglycerate dehydrogenase (3-PGDH)
2	2335	5 (12)	13.0	12.5	-14.3	37.7		54304/8.3		MOUSE	Gamma-aminobutyric-acid receptor gamma-3 subunit precursor (GABA(A) receptor)
3	1499	6 (15)	14.0	15.0	-16.0	51.5		65979/8.4		MOUSE	G protein-coupled receptor kinase GRK6 (G-protein-coupled receptor kinase 6)
4	1195	5 (12)	13.0	12.5	11.4	19.8		51449/6.5		MOUSE	D-3-phosphoglycerate dehydrogenase (3-PGDH) (A10)
5	733	4 (10)	20.0	10.0	20.4	22.8		38033/7.1		MOUSE	GMP reductase 2 (Guanosine 5'-monophosphate oxidoreductase 2) (Guanosine monophosphate reductase 2)

39398	x	.	.	x	x	.	x	x	x	.	x	x	x	x	.	.	.	.	.	.	.	.	.	x	.	.	x	.	x	.	.	.	x	.	.	.	.	x	x	.
35936	.	.	.	.	.	.	x	.	.	.	.	.	x	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	x	.	.	.	.	.	.
1267	.	.	.	x	.	.	x	.	.	.	.	x	.	x	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.
91099	.	.	.	x	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	x	.	x	.	.	.	.	.	.	.	.	.	x	.
80793	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	x	.

Detailed Results

1. 16/40 matches (40%).
Acc. #: Species: HUMAN Name: D-3-phosphoglycerate dehydrogenase (3-PGDH)
Index: MW: 56651 Da pI: 6.3

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
986.6160	986.6039	12		462	469	0	(R) (T)
1099.6290	1099.6111	16		237	247	0	(R) (A)
1345.7606	1345.7691	-6.3		352	364	0	(K) (N)
1390.6678	1390.6544	9.6		523	533	0	(K) (-)
1390.6678	1390.7000	-23	1Met-ox	120	132	1	(R)QIPQATASMKDGK(W)
1426.7855	1426.8270	-29		21	33	1	(R) (Q)
1462.7190	1462.7034	11		9	20	0	(K) (K)
1488.7536	1488.7294	16		76	90	0	(R) (K)
1590.8235	1590.7984	16		8	20	1	(R) (K)
1590.8235	1590.7984	16		9	21	1	(K) (I)
1639.9281	1639.9205	4.6		365	380	0	(K) (E)
1654.8056	1654.7821	14	1Met-ox	295	308	0	(R)CGEEIAVQFVDMVK(G)
1900.9746	1900.9690	2.9		39	54	0	(K) (S)
2062.0387	2062.0391	-0.22		271	289	0	(R) (E)
2231.1297	2231.0767	24		248	268	0	(R) (D)
2304.1795	2304.1579	9.4	2Met-ox	470	491	0	(R)TQTSDPAMLPTMIGLLAEAGVR(L)
2471.2738	2471.2816	-3.1		34	54	1	(K) (S)
2502.2118	2502.2047	2.8		248	270	1	(R) (A)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 39% (212/533AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 5/40 matches (12%).
Acc. #: Species: MOUSE Name: Gamma-aminobutyric-acid receptor gamma-3 subunit precursor (GABA(A) receptor)
Index: MW: 54304 Da pI: 8.3

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1390.6678 1390.6715 -2.7 206 217 1 (K) (L)
1639.9281 1639.9722 -27 5 18 0 (K) (S)
1746.9010 1746.8775 13 432 446 1 (R) (V)
2304.1795 2304.2426 -27 115 134 0 (K) (N)
2320.1727 2320.2375 -28 1Met-ox 115 134 0 (K)TLTLNSNMVGLIWIPDTIFR(N)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 13% (61/467AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 6/40 matches (15%).
Acc. #: Species: MOUSE Name: G protein-coupled receptor kinase GRK6 (G-protein-coupled receptor kinase 6)
Index: MW: 65979 Da pI: 8.4

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1099.6290 1099.6740 -41 60 68 1 (R) (E)
1390.6678 1390.7258 -42 245 256 0 (R) (D)
1590.8235 1590.8426 -12 344 358 1 (K) (N)
1654.8056 1654.7601 28 488 501 0 (K) (F)
1746.9010 1746.9134 -7.1 257 272 0 (K) (F)
2320.1727 2320.2236 -22 104 123 1 (R) (Q)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 14% (86/576AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 5/40 matches (12%).
Acc. #: Species: MOUSE Name: D-3-phosphoglycerate dehydrogenase (3-PGDH) (A10)
Index: MW: 51449 Da pI: 6.5

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1099.6290 1099.6111 16 189 199 0 (R) (A)
1654.8056 1654.7821 14 1Met-ox 247 260 0 (R)CGEEIAVQFVDMVK(G)
2062.0387 2062.0391 -0.22 223 241 0 (R) (E)
2231.1297 2231.0767 24 200 220 0 (R) (D)
2502.2118 2502.2047 2.8 200 222 1 (R) (A)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 13% (67/485AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 4/40 matches (10%).
Acc. #: Species: MOUSE Name: GMP reductase 2 (Guanosine 5'-monophosphate oxidoreductase 2) (Guanosine monophosphate reductase 2)
Index: MW: 38033 Da pI: 7.1

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1345.7606 1345.7262 26 178 190 1 (K) (K)
1795.9566 1795.9376 11 307 323 1 (R) (L)
2304.1795 2304.1011 34 125 143 0 (K) (D)
2502.2118 2502.1831 11 43 65 0 (K) (V)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 20% (72/348AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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