MS-Fit Search Results


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Parameters

Database searched: SwissProt.01.22.2003
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): 40-43
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 121920
Molecular weight search (1000 - 100000 Da) selects 115277 entries.
Full pI range: 121920 entries.
Species search ( HUMAN MOUSE ) selects 14893 entries.
Combined molecular weight, pI and species searches select 13163 entries.
Pre searches select 13163 entries.

Data Set 1 Results

MS-Fit search selects 1416 entries (results displayed for top 5 matches).

Results Summary

MOWSE
Score		#/90(%)
Masses
Matched 		%
Cov		%
TIC		Mean
Err
ppm		Data
Tol
ppm		MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
11.070e+00714 (15)32.015.6-11.831.4 55367/6.2HUMANAldehyde dehydrogenase family 7 member A1 (Antiquitin 1)
22.494e+00510 (11)21.011.1-8.1347.3 52772/6.3HUMANCytosol aminopeptidase (Leucine aminopeptidase) (LAP) (Leucyl aminopeptidase) (Proline aminopeptidase) (Prolyl aminopeptidase)
32.204e+0059 (10)22.010.0-13.853.4 66018/8.2HUMANKeratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
48.461e+00411 (12)24.012.2-9.9552.0 73244/6.7HUMANDEAD-box protein 3 (Helicase-like protein 2) (HLP2) (DEAD-box, X isoform)
54.174e+00411 (12)22.012.2-8.1855.1 73141/6.7MOUSEPutative ATP-dependent RNA helicase PL10
62902.........xx....x...............x....x........xx........xxxx........x...x..............x...
99266.....x..........xx.x............x.......x.x..x.................................x......x...
48753...........x..x........x..........x......x..x.x................x...................x......
3614.........x.........x....x.xx.................x........x.x....x....x..................x....
105637.........x.........x....x.xx.................x.x......x.x....x....x.......................


Detailed Results

1. 14/90 matches (15%).
Acc. #: Species: HUMAN Name: Aldehyde dehydrogenase family 7 member A1 (Antiquitin 1)
Index: MW: 55367 Da pI: 6.2
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1058.51161058.5305-18pyroGlu 1Met-ox 2532610(K)QVGLMVQER(F)
1075.53031075.5570-251Met-ox 2532610(K)QVGLMVQER(F)
1185.59881185.6302-261Met-ox 1351450(R)MIGGPILPSER(S)
1435.71891435.7480-201Met-ox 2532641(K)QVGLMVQERFGR(S)
1553.66721553.66213.321340(R) (G)
1707.83681707.8818-263083210(R) (L)
1716.89431716.9325-22AcetN 1150(-)STLLINQPQYAWLK(E)
1863.93451863.9830-263073211(R) (L)
1882.89921882.8612204104240(K) (Q)
1906.98411906.98161.33313470(R) (Q)
1918.92111918.9333-6.435510(R) (V)
2091.99772092.0241-132152350(K) (D)
2122.02472121.99541416341(K) (G)
2697.28832697.3446-211111340(K) (M)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 32% (166/511AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 10/90 matches (11%).
Acc. #: Species: HUMAN Name: Cytosol aminopeptidase (Leucine aminopeptidase) (LAP) (Leucyl aminopeptidase) (Proline aminopeptidase) (Prolyl aminopeptidase)
Index: MW: 52772 Da pI: 6.3
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1004.58301004.6144-314754820(R) (F)
1193.56591193.5777-9.94014090(R) (Q)
1209.52851209.5727-361Met-ox 4014090(R)MPLFEHYTR(Q)
1232.63831232.6751-301581690(K) (Q)
1439.77091439.7971-181942061(K) (S)
1630.80241630.8117-5.72232360(K) (G)
1650.83161650.82156.11Met-ox 3984091(R)VWRMPLFEHYTR(Q)
1707.83681707.8410-2.52732900(R) (L)
2281.20052281.09884515351(K) (L)
2697.28832697.28321.92642901(K) (L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 21% (107/488AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 9/90 matches (10%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
Index: MW: 66018 Da pI: 8.2
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1125.49001125.5428-473673760(K) (Y)
1179.56081179.6010-343773860(K) (H)
1277.66361277.6337232472571(K) (N)
1277.66361277.7105-374734830(K) (T)
1475.69811475.7494-352122230(K) (T)
1638.84381638.8604-101861991(K) (F)
1687.91521687.9707-3366821(R) (G)
1716.89431716.8517254184320(K) (G)
1993.98681993.97724.82242390(R) (R)
2383.96352383.95244.65195490(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 22% (147/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 11/90 matches (12%).
Acc. #: Species: HUMAN Name: DEAD-box protein 3 (Helicase-like protein 2) (HLP2) (DEAD-box, X isoform)
Index: MW: 73244 Da pI: 6.7
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1058.51161058.5159-4.047551(R) (D)
1232.63831232.5812465936031(R) (Q)
1300.59601300.6398-344794881(R) (S)
1313.71921313.6887233633731(R) (G)
1320.62821320.6800-392772870(R) (K)
1707.83681707.8964-353123261(R) (L)
1838.93621838.9513-8.25325481(R) (N)
1882.89921882.9187-101461620(R) (Y)
1940.92071940.9792-303363511(K) (M)
2083.00922083.0249-7.55125280(K) (I)
2662.39892662.4278-112312551(K) (E)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 24% (159/662AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 11/90 matches (12%).
Acc. #: Species: MOUSE Name: Putative ATP-dependent RNA helicase PL10
Index: MW: 73141 Da pI: 6.7
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1058.51161058.49072051591(K) (W)
1232.63831232.5812465916011(R) (Q)
1300.59601300.6398-344784871(R) (S)
1313.71921313.6887233623721(R) (G)
1320.62821320.6800-392762860(R) (K)
1707.83681707.8964-353113251(R) (L)
1765.74741765.7741-155996151(R) (A)
1838.93621838.9513-8.25315471(R) (N)
1882.89921882.9187-101451610(R) (Y)
1940.92071940.9792-303353501(K) (M)
2083.00922083.0249-7.55115270(K) (I)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 22% (148/660AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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