MS-Fit Search Results



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Parameters

Database searched: SwissProt.01.22.2003
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): 40-51
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 121920
Molecular weight search (1000 - 100000 Da) selects 115277 entries.
Full pI range: 121920 entries.
Species search ( HUMAN MOUSE ) selects 14893 entries.
Combined molecular weight, pI and species searches select 13163 entries.
Pre searches select 13163 entries.

Data Set 1 Results

MS-Fit search selects 574 entries (results displayed for top 5 matches).

Results Summary

MOWSE
Score
#/60(%)
Masses
Matched
%
Cov
%
TIC
Mean
Err
ppm
Data
Tol
ppm
MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
16.395e+00715 (25)40.025.0-15.036.5 55805/6.4HUMANInosine-5'-monophosphate dehydrogenase 2 (IMP dehydrogenase 2) (IMPDH-II) (IMPD 2)
25.366e+00510 (16)26.016.7-12.243.8 55785/6.8MOUSEInosine-5'-monophosphate dehydrogenase 2 (IMP dehydrogenase 2) (IMPDH-II) (IMPD 2)
31.286e+00510 (16)23.016.7-18.826.4 54390/7.5HUMANAnnexin A11 (Annexin XI) (Calcyclin-associated annexin 50) (CAP-50) (56 kDa autoantigen)
491629 (15)14.015.0-18.647.5 96042/4.8MOUSENeurofilament triplet M protein (160 kDa neurofilament protein) (Neurofilament medium polypeptide) (NF-M)
539837 (11)18.011.7-21.151.4 66018/8.2HUMANKeratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
82365.x.......x.........x.x..x......x..x...x.x..xx....xxx......x.
76927.x.......x.........x.x............x...x.x..x.....x.x........
85469..x..x..........x..x..x.......x..x....x.........x........x..
78492.xx.......x..x....x................x.x.........x........x...
48753..........xx..x........x.....x...x.............x............



Detailed Results

1. 15/60 matches (25%).
Acc. #: Species: HUMAN Name: Inosine-5'-monophosphate dehydrogenase 2 (IMP dehydrogenase 2) (IMPDH-II) (IMPD 2)
Index: MW: 55805 Da pI: 6.4
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
972.4872972.5114-251962030(K) (S)
1156.60831156.6326-213123220(K) (V)
1359.66681359.6037461Met-ox 4114221(K)YRGMGSLDAMDK(H)
1444.67641444.7007-171371490(R) (M)
1481.83241481.8692-251821951(K) (E)
1779.94901779.9856-212092240(K) (T)
1820.93151820.9798-271091241(K) (D)
1891.98691892.0394-283573750(R) (A)
1915.89531915.9150-104945110(R) (R)
1960.94021960.9551-7.62432591(K) (L)
1965.05641965.1021-232072241(K) (T)
2048.09142048.1405-243563751(R) (A)
2086.08272086.1297-232923111(K) (N)
2131.08002131.1010-9.94564740(K) (S)
2571.13462571.1635-111Met-ox 1621811(R)DIDFLKEEEHDCFLEEIMTK(R)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 40% (206/514AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 10/60 matches (16%).
Acc. #: Species: MOUSE Name: Inosine-5'-monophosphate dehydrogenase 2 (IMP dehydrogenase 2) (IMPDH-II) (IMPD 2)
Index: MW: 55785 Da pI: 6.8
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
972.4872972.5114-251962030(K) (S)
1156.60831156.6326-213123220(K) (V)
1359.66681359.6037461Met-ox 4114221(K)YRGMGSLDAMDK(H)
1444.67641444.7007-171371490(R) (M)
1820.93151820.9798-271091241(K) (D)
1891.98691892.0394-283573750(R) (A)
1915.89531915.9150-104945110(R) (R)
1960.94021960.9551-7.62432591(K) (L)
2048.09142048.1405-243563751(R) (A)
2131.08002131.1010-9.94564740(K) (S)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 26% (134/514AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 10/60 matches (16%).
Acc. #: Species: HUMAN Name: Annexin A11 (Annexin XI) (Calcyclin-associated annexin 50) (CAP-50) (56 kDa autoantigen)
Index: MW: 54390 Da pI: 7.5
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
980.4742980.4988-253793860(K) (S)
1052.49701052.5165-194314390(K) (L)
1336.59541336.6133-133603710(R) (L)
1359.66681359.6909-181922040(R) (D)
1446.71431446.7494-243894000(R) (M)
1717.83921717.8795-232873020(K) (S)
1813.80631813.8357-164804951(K) (I)
1891.98691891.9601143894041(R) (D)
2042.98472043.0511-321922101(R) (K)
2563.19752563.2786-321Met-ox 3373591(R)LLISLSQGNRDESTNVDMSLAQR(D)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 23% (119/505AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 9/60 matches (15%).
Acc. #: Species: MOUSE Name: Neurofilament triplet M protein (160 kDa neurofilament protein) (Neurofilament medium polypeptide) (NF-M)
Index: MW: 96042 Da pI: 4.8
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
972.4872972.463824pyroGlu 3433500(R)QLSDIEER(H)
980.4742980.4801-6.028370(R) (S)
1159.60601159.6071-0.941942031(R) (A)
1273.59411273.6276-267317420(K) (E)
1339.65821339.7222-486977071(K) (E)
1836.82371836.9119-484524661(K) (S)
1880.92791880.9507-121021171(K) (V)
1993.93921993.9640-124674850(K) (E)
2502.16422502.2589-382362570(R) (K)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 14% (123/849AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 7/60 matches (11%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
Index: MW: 66018 Da pI: 8.2
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1159.60601159.6547-4275861(K) (G)
1179.57221179.6010-243773860(K) (H)
1277.68321277.6337392472571(K) (N)
1277.68321277.7105-214734830(K) (T)
1475.72421475.7494-172122230(K) (T)
1475.72421475.7858-422002110(R) (W)
1716.82791716.8517-144184320(K) (G)
1813.80631813.8945-4913301(R) (T)
1993.93921993.9772-192242390(R) (R)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 18% (117/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):


MS-Fit in ProteinProspector 4.0.4
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