MS-Fit Search Results

MS-Fit Search Results

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Parameters

Database searched: SwissProt.01.22.2003
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): 40-53
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 121920
Molecular weight search (1000 - 100000 Da) selects 115277 entries.
Full pI range: 121920 entries.
Species search ( HUMAN MOUSE ) selects 14893 entries.
Combined molecular weight, pI and species searches select 13163 entries.
Pre searches select 13163 entries.

Data Set 1 Results

MS-Fit search selects 484 entries (results displayed for top 5 matches).

Results Summary

	MOWSE Score	#/60(%) Masses Matched	% Cov	% TIC	Mean Err ppm	Data Tol ppm	MS-Digest Index #	Protein MW (Da)/pI	Accession #	Species	Protein Name
1	1.053e+008	13 (21)	42.0	21.7	-25.2	32.0		54530/6.8		HUMAN	Fascin (Singed-like protein) (55 kDa actin bundling protein) (p55)
2	2.431e+004	6 (10)	15.0	10.0	-23.8	46.8		47232/4.9		HUMAN	Keratin, type I cuticular HA1 (Hair keratin, type I HA1)
3	7931	6 (10)	12.0	10.0	4.40	52.6		78539/7.0		MOUSE	Acyl-coenzyme A oxidase 3, peroxisomal (Pristanoyl-CoA oxidase)
4	7653	7 (11)	12.0	11.7	1.57	58.0		47315/6.5		HUMAN	Transcription factor SOX-8
5	5810	7 (11)	14.0	11.7	-9.68	59.6		69900/6.3		HUMAN	Baculoviral IAP repeat-containing protein 3 (Inhibitor of apoptosis protein 2) (HIAP2) (HIAP-2) (C-IAP1) (TNFR2-TRAF signaling complex protein 2) (IAP homolog B)

120748	.	.	.	.	.	x	.	.	.	.	x	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	x	.	.	x	.	.	.	.	.	.	x	x	.	.	x	.	.	.	x	x	.	.	x	.	.	x
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Detailed Results

1. 13/60 matches (21%).
Acc. #: Species: HUMAN Name: Fascin (Singed-like protein) (55 kDa actin bundling protein) (p55)
Index: MW: 54530 Da pI: 6.8

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
1046.4439	1046.4842	-38		390	398	0	(R) (K)
1113.5053	1113.5441	-35		101	109	0	(R) (R)
1240.7376	1240.7894	-42		380	389	0	(K) (G)
1474.6093	1474.6425	-23		331	341	0	(K) (D)
1625.7863	1625.7593	17		69	82	1	(R) (E)
1819.9155	1819.9707	-30		202	217	0	(R) (S)
2059.9810	2060.0453	-31		168	185	0	(R) (Y)
2109.9431	2109.9769	-16		409	426	0	(R) (D)
2154.0067	2154.0919	-40	1Met-ox	132	149	0	(K)WSVHIAMHPQVNIYSVTR(K)
2225.0563	2225.1124	-25	1Met-ox	359	379	1	(K)KNGQLAASVETAGDSELFLMK(L)
2255.0770	2255.1130	-16		44	63	0	(K) (S)
2351.1227	2351.1488	-11		1	22	0	(-) (Y)
2689.2613	2689.3619	-37		248	271	1	(K) (N)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 42% (210/493AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 6/60 matches (10%).
Acc. #: Species: HUMAN Name: Keratin, type I cuticular HA1 (Hair keratin, type I HA1)
Index: MW: 47232 Da pI: 4.9

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1124.5235 1124.4981 23 408 416 0 (R) (-)
1153.4917 1153.5312 -34 58 66 0 (K) (L)
1487.6919 1487.7494 -39 pyroGlu 335 346 0 (R)QNQEYQVLLDVR(A)
1504.7342 1504.7760 -28 335 346 0 (R) (A)
1625.7863 1625.8499 -39 209 223 0 (R) (V)
2307.1237 2307.1832 -26 257 276 0 (K) (R)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 15% (65/416AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 6/60 matches (10%).
Acc. #: Species: MOUSE Name: Acyl-coenzyme A oxidase 3, peroxisomal (Pristanoyl-CoA oxidase)
Index: MW: 78539 Da pI: 7.0

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1124.5235 1124.5336 -9.0 610 620 0 (R) (A)
1435.7141 1435.7334 -13 658 669 1 (K) (V)
1449.6904 1449.6367 37 424 436 0 (R) (F)
1625.7863 1625.7746 7.2 251 265 0 (K) (V)
1650.8276 1650.8743 -28 485 499 0 (K) (F)
2637.4514 2637.3645 33 548 570 0 (R) (F)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 12% (89/700AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 7/60 matches (11%).
Acc. #: Species: HUMAN Name: Transcription factor SOX-8
Index: MW: 47315 Da pI: 6.5

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
863.3763 863.3933 -20 AcetN 1 8 0 (-)LDMSEAR(S)
952.4537 952.4232 32 1 8 0 (-) (S)
968.4560 968.4181 39 1Met-ox 1 8 0 (-)MLDMSEAR(S)
1419.7120 1419.7095 1.8 78 89 0 (K) (G)
1435.7141 1435.7044 6.8 1Met-ox 78 89 0 (K)GYDWSLVPMPVR(G)
1918.9804 1918.9874 -3.6 142 157 1 (R) (L)
1987.9301 1988.0201 -45 320 339 0 (K) (T)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 12% (56/446AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 7/60 matches (11%).
Acc. #: Species: HUMAN Name: Baculoviral IAP repeat-containing protein 3 (Inhibitor of apoptosis protein 2) (HIAP2) (HIAP-2) (C-IAP1) (TNFR2-TRAF signaling complex protein 2) (IAP homolog B)
Index: MW: 69900 Da pI: 6.3

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
968.4560 968.4941 -39 530 537 0 (K) (N)
1124.5235 1124.5774 -48 1Met-ox 538 546 1 (K)NLFVDKNMK(Y)
1263.6498 1263.6737 -19 9 19 0 (R) (S)
1277.6476 1277.6126 27 341 351 0 (K) (Y)
1834.9298 1834.8857 24 447 462 1 (K) (K)
1940.9048 1940.8911 7.0 20 36 0 (K) (Q)
1987.9301 1987.9700 -20 243 258 0 (R) (F)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 14% (88/618AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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