MS-Fit Search Results



.

Parameters

Database searched: SwissProt.01.22.2003
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): 40-54
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 121920
Molecular weight search (10000 - 100000 Da) selects 104019 entries.
Full pI range: 121920 entries.
Species search ( HUMAN MOUSE ) selects 14893 entries.
Combined molecular weight, pI and species searches select 12734 entries.
Pre searches select 12734 entries.

Data Set 1 Results

MS-Fit search selects 1692 entries (results displayed for top 5 matches).

Results Summary

MOWSE
Score
#/78(%)
Masses
Matched
%
Cov
%
TIC
Mean
Err
ppm
Data
Tol
ppm
MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
11.358e+00918 (23)45.023.116.623.9 47169/7.0HUMANAlpha enolase (2-phospho-D-glycerate hydro-lyase) (Non-neural enolase) (NNE) (Enolase 1) (Phosphopyruvate hydratase)
22.860e+00611 (14)36.014.113.137.9 47141/6.4MOUSEAlpha enolase (2-phospho-D-glycerate hydro-lyase) (Non-neural enolase) (NNE) (Enolase 1)
31.242e+00612 (15)26.015.48.6940.0 66018/8.2HUMANKeratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
42.845e+0048 (10)22.010.311.619.5 47025/6.7MOUSEBeta enolase (2-phospho-D-glycerate hydro-lyase) (Skeletal muscle enolase) (Enolase 3)
51.590e+0047 (8)19.09.013.019.2 46987/7.6HUMANBeta enolase (2-phospho-D-glycerate hydro-lyase) (Skeletal muscle enolase) (MSE) (Enolase 3)
65205..x..x...x.x................x..x............xx.xx..xx.....x....x.....xx.x.x...
41608.....x...x.x................x................x..x..x......x....x........x.x...
48753.x.xx.........x.....xx.....x......x...........x.............x............x..x.
10462......x...........................x..........x..x...x.x...x....x..............
27136......x......................................x..x...x.x...x....x..............



Detailed Results

1. 18/78 matches (23%).
Acc. #: Species: HUMAN Name: Alpha enolase (2-phospho-D-glycerate hydro-lyase) (Non-neural enolase) (NNE) (Enolase 1) (Phosphopyruvate hydratase)
Index: MW: 47169 Da pI: 7.0
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
959.5274959.5427-164274341(R) (-)
1035.54151035.53318.13363430(K) (V)
1072.55491072.5315222542621(R) (S)
1143.63171143.6162141841930(R) (N)
1406.73421406.71671216280(R) (G)
1425.74831425.7266152702810(R) (S)
1525.77261525.76981.93593720(K) (S)
1533.85311533.84634.41061200(K) (A)
1540.80511540.7834142402530(K) (S)
1541.79561541.7647201Met-ox 3593720(K)LAQANGWGVMVSHR(S)
1554.74881554.7076262572691(K) (Y)
1556.80821556.7783191Met-ox 2402530(K)VVIGMDVAASEFFR(S)
1647.86471647.8376163443580(K) (L)
1804.98261804.94452133500(R) (D)
1924.04121923.9825311Met-ox 1631790(K)LAMQEFMILPVGAANFR(E)
1940.04311939.9774342Met-ox 1631790(K)LAMQEFMILPVGAANFR(E)
1960.97721960.9252272032210(K) (E)
2033.11342033.0555283073260(K) (R)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 45% (199/434AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 11/78 matches (14%).
Acc. #: Species: MOUSE Name: Alpha enolase (2-phospho-D-glycerate hydro-lyase) (Non-neural enolase) (NNE) (Enolase 1)
Index: MW: 47141 Da pI: 6.4
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1035.54151035.53318.13363430(K) (V)
1072.55491072.5315222542621(R) (S)
1072.55491072.6002-4281891(K) (I)
1143.63171143.6162141841930(R) (N)
1406.73421406.71671216280(R) (G)
1533.85311533.84634.41061200(K) (A)
1541.79561541.7647203593720(K) (S)
1554.74881554.7076262572691(K) (Y)
1647.86471647.8376163443580(K) (L)
1804.98261804.94452133500(R) (D)
1960.97721960.9252272032210(K) (E)
2033.11342033.0555283073260(K) (R)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 36% (157/434AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 12/78 matches (15%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
Index: MW: 66018 Da pI: 8.2
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
945.4562945.5005-474334411(R) (N)
973.5397973.53188.13964030(K) (V)
1033.54221033.5166254844920(R) (M)
1179.62061179.6010173773860(K) (H)
1277.73401277.7105184734830(K) (T)
1302.70041302.7018-1.03934031(R) (V)
1302.70041302.7157-123443550(R) (A)
1383.71391383.6909171861970(K) (V)
1475.78811475.7494262122230(K) (T)
1475.78811475.78581.62002110(R) (W)
1539.78251539.7841-1.11Met-ox 4614721(R)LLRDYQELMNTK(L)
1716.89311716.8517244184320(K) (G)
1994.02741993.9772252242390(R) (R)
2384.00202383.9524215195490(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 26% (172/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 8/78 matches (10%).
Acc. #: Species: MOUSE Name: Beta enolase (2-phospho-D-glycerate hydro-lyase) (Skeletal muscle enolase) (Enolase 3)
Index: MW: 47025 Da pI: 6.7
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1045.58451045.5893-4.681891(K) (V)
1475.78811475.78581.64134261(R) (K)
1533.85311533.84634.41061200(K) (A)
1541.79561541.7647203593720(K) (S)
1556.80821556.7783192402530(K) (N)
1572.79631572.7732151Met-ox 2402530(K)VVIGMDVAASEFYR(N)
1647.86471647.8376163443580(K) (L)
1804.98261804.94452133500(R) (D)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 22% (99/434AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 7/78 matches (8%).
Acc. #: Species: HUMAN Name: Beta enolase (2-phospho-D-glycerate hydro-lyase) (Skeletal muscle enolase) (MSE) (Enolase 3)
Index: MW: 46987 Da pI: 7.6
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1045.58451045.5893-4.681891(K) (V)
1533.85311533.84634.41061200(K) (A)
1541.79561541.7647203593720(K) (S)
1556.80821556.7783192402530(K) (N)
1572.79631572.7732151Met-ox 2402530(K)VVIGMDVAASEFYR(N)
1647.86471647.8376163443580(K) (L)
1804.98261804.94452133500(R) (D)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 19% (85/434AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):


MS-Fit in ProteinProspector 4.0.4
© Copyright (1995-2002) The Regents of the University of California.