MS-Fit Search Results


.

Parameters

Database searched: SwissProt.01.22.2003
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): 40-55
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 121920
Molecular weight search (1000 - 100000 Da) selects 115277 entries.
Full pI range: 121920 entries.
Species search ( HUMAN MOUSE ) selects 14893 entries.
Combined molecular weight, pI and species searches select 13163 entries.
Pre searches select 13163 entries.

Data Set 1 Results

MS-Fit search selects 1559 entries (results displayed for top 5 matches).

Results Summary

MOWSE
Score		#/80(%)
Masses
Matched 		%
Cov		%
TIC		Mean
Err
ppm		Data
Tol
ppm		MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
12.033e+01022 (27)54.027.544.240.8 49542/7.3HUMANElongation factor Tu, mitochondrial precursor (EF-Tu) (P43)
21.226e+0059 (11)30.011.354.921.3 41737/5.3HUMANActin, cytoplasmic 1 (Beta-actin)
31.225e+0059 (11)30.011.354.921.3 41793/5.3HUMANActin, cytoplasmic 2 (Gamma-actin)
42.765e+0048 (10)19.010.033.452.7 46660/6.5HUMANIsocitrate dehydrogenase [NADP] cytoplasmic (Oxalosuccinate decarboxylase) (IDH) (NADP+-specific ICDH) (IDP)
51.545e+0049 (11)15.011.39.3166.9 68585/9.2MOUSELymphoid BLAST crisis-like 1 (LBC'S first cousin) (Oncogene LFC) (RHOBIN)
120900.xx.....x..............x...x..x....x..x..x.x....xxx........x....xx.xxx.....xxx..
57770.............................x......x..............x...xx...........x...xx.....x
118938.............................x......x..............x...xx...........x...xx.....x
76619.........x.x....x....x.........xx.............x.......................x.........
45089........x.x..x.x.......x..........................x..........x....x......x......


Detailed Results

1. 22/80 matches (27%).
Acc. #: Species: HUMAN Name: Elongation factor Tu, mitochondrial precursor (EF-Tu) (P43)
Index: MW: 49542 Da pI: 7.3
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
851.5477851.5103441631690(R) (Q)
857.4717857.444332pyroGlu 1Met-ox 4124180(R)QPMILEK(G)
874.5053874.4708391Met-ox 4124180(R)QPMILEK(G)
1032.61801032.5689482722810(R) (G)
1099.59231099.5676222012090(R) (G)
1149.70821149.6519493523610(K) (E)
1185.68951185.6228563163270(R) (G)
1277.72391277.6564531Met-ox 3013110(R)TVVTGIEMFHK(S)
1364.67691364.597059921020(K) (A)
1386.71191386.6687312862971(K) (N)
1466.88901466.800760pyroGlu 1701820(R)QIGVEHVVVYVNK(A)
1483.90741483.8233573163301(R) (R)
1483.90741483.8273541701820(R) (A)
1492.77361492.692055911021(K) (A)
1542.93091542.8532502392520(K) (D)
1673.95141673.8611541051200(R) (H)
1677.94131677.8812363523661(K) (H)
1784.89461784.7815631211350(R) (N)
1790.99081791.0427-291Met-ox 3363511(R)RGLVMVKPGSIKPHQK(V)
1810.02271809.93604854700(R) (T)
2012.12502012.0864191201(-) (T)
2062.14712062.0014711Met-ox 1832000(K)ADAVQDSEMVELVELEIR(E)
2129.26372129.1647472532710(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 54% (246/452AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 9/80 matches (11%).
Acc. #: Species: HUMAN Name: Actin, cytoplasmic 1 (Beta-actin)
Index: MW: 41737 Da pI: 5.3
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1132.58621132.5275521972060(R) (E)
1198.76961198.70615329390(R) (H)
1499.77501499.676766pyroGlu 3603720(K)QEYDESGPSIVHR(K)
1515.79451515.74973085950(K) (V)
1516.79561516.7032613603720(K) (K)
1790.99081790.8925552392540(K) (F)
1954.19131954.065065961130(R) (A)
1963.00221962.8908571Met-ox 69840(K)YPIEHGIVTNWDDMEK(I)
2231.19232231.0654571Met-ox 2923120(K)DLYANTVLSGGTTMYPGIADR(M)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 30% (116/375AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 9/80 matches (11%).
Acc. #: Species: HUMAN Name: Actin, cytoplasmic 2 (Gamma-actin)
Index: MW: 41793 Da pI: 5.3
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1132.58621132.5275521972060(R) (E)
1198.76961198.70615329390(R) (H)
1499.77501499.676766pyroGlu 3603720(K)QEYDESGPSIVHR(K)
1515.79451515.74973085950(K) (V)
1516.79561516.7032613603720(K) (K)
1790.99081790.8925552392540(K) (F)
1954.19131954.065065961130(R) (A)
1963.00221962.8908571Met-ox 69840(K)YPIEHGIVTNWDDMEK(I)
2231.19232231.0654571Met-ox 2923120(K)DLYANTVLSGGTTMYPGIADR(M)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 30% (116/375AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 8/80 matches (10%).
Acc. #: Species: HUMAN Name: Isocitrate dehydrogenase [NADP] cytoplasmic (Oxalosuccinate decarboxylase) (IDH) (NADP+-specific ICDH) (IDP)
Index: MW: 46660 Da pI: 6.5
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
903.4777903.4477332442490(K) (L)
914.6038914.5675404014081(K) (L)
914.6038914.57153521270(R) (E)
976.5699976.5580121011090(R) (E)
1009.50871009.4492591331400(R) (A)
1168.60161168.5461472612700(K) (N)
1171.68051171.7091-2421291(R) (L)
1437.94511437.8946351201320(R) (H)
1879.05311878.9350633223380(K) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 19% (80/414AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 9/80 matches (11%).
Acc. #: Species: MOUSE Name: Lymphoid BLAST crisis-like 1 (LBC'S first cousin) (Oncogene LFC) (RHOBIN)
Index: MW: 68585 Da pI: 9.2
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
874.5053874.5151-113954010(K) (I)
905.5288905.52425.03703771(K) (R)
935.4350935.4951-644434490(R) (M)
945.5839945.5482384111(R) (I)
1032.61801032.5842332943010(R) (R)
1492.77361492.7430211691810(R) (N)
1566.83311566.8426-6.13793910(R) (I)
1767.95021767.8765424254390(K) (E)
1963.00221962.9483275735881(R) (A)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 15% (95/596AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
© Copyright (1995-2002) The Regents of the University of California.