MS-Fit Search Results


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Parameters

Database searched: SwissProt.01.22.2003
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): 40-57
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 121920
Molecular weight search (1000 - 100000 Da) selects 115277 entries.
Full pI range: 121920 entries.
Species search ( HUMAN MOUSE ) selects 14893 entries.
Combined molecular weight, pI and species searches select 13163 entries.
Pre searches select 13163 entries.

Data Set 1 Results

MS-Fit search selects 437 entries (results displayed for top 5 matches).

Results Summary

MOWSE
Score		#/60(%)
Masses
Matched 		%
Cov		%
TIC		Mean
Err
ppm		Data
Tol
ppm		MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
14.990e+01117 (28)49.028.324.412.0 47169/7.0HUMANAlpha enolase (2-phospho-D-glycerate hydro-lyase) (Non-neural enolase) (NNE) (Enolase 1) (Phosphopyruvate hydratase)
21.073e+0068 (13)27.013.325.320.2 47141/6.4MOUSEAlpha enolase (2-phospho-D-glycerate hydro-lyase) (Non-neural enolase) (NNE) (Enolase 1)
34.304e+0046 (10)20.010.018.819.1 47269/4.9HUMANGamma enolase (2-phospho-D-glycerate hydro-lyase) (Neural enolase) (NSE) (Enolase 2)
42.678e+0047 (11)18.011.716.656.2 49478/5.8HUMANAlpha enolase, lung specific (2-phospho-D-glycerate hydro-lyase) (Non-neural enolase) (NNE) (Phosphopyruvate hydratase) (HLE1)
52.533e+0048 (13)10.013.36.1748.9 96950/8.2MOUSEDNA topoisomerase III beta-1
65205x..x.xx......x.xx...x....xx..............x.x.x.....xx..x.x..
41608x..x.x.........x.........xx........................xx.......
110904.....x.........x......x.x.x.................x...............
27268...x..x.............x..x.x.x................x...............
57521...x..........x.x.x.x......xx..................x............


Detailed Results

1. 17/60 matches (28%).
Acc. #: Species: HUMAN Name: Alpha enolase (2-phospho-D-glycerate hydro-lyase) (Non-neural enolase) (NNE) (Enolase 1) (Phosphopyruvate hydratase)
Index: MW: 47169 Da pI: 7.0
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1035.55441035.5331213363430(K) (V)
1143.64221143.6162231841930(R) (N)
1406.73331406.71671216280(R) (G)
1425.76541425.7266272702810(R) (S)
1525.78641525.7698113593720(K) (S)
1533.88561533.8463261061200(K) (A)
1540.82251540.7834252402530(K) (S)
1556.81901556.7783261Met-ox 2402530(K)VVIGMDVAASEFFR(S)
1647.87671647.8376243443580(K) (L)
1804.98981804.94452533500(R) (D)
1908.03001907.9876221631790(K) (E)
1924.02851923.9825241Met-ox 1631790(K)LAMQEFMILPVGAANFR(E)
1940.03571939.9774302Met-ox 1631790(K)LAMQEFMILPVGAANFR(E)
2033.10712033.0555253073260(K) (R)
2192.13772192.0698311Met-ox 2342531(K)AGYTDKVVIGMDVAASEFFR(S)
2510.18842510.1152292863060(K) (F)
3011.67393011.5703341331620(R) (L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 49% (213/434AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 8/60 matches (13%).
Acc. #: Species: MOUSE Name: Alpha enolase (2-phospho-D-glycerate hydro-lyase) (Non-neural enolase) (NNE) (Enolase 1)
Index: MW: 47141 Da pI: 6.4
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1035.55441035.5331213363430(K) (V)
1143.64221143.6162231841930(R) (N)
1406.73331406.71671216280(R) (G)
1533.88561533.8463261061200(K) (A)
1647.87671647.8376243443580(K) (L)
1804.98981804.94452533500(R) (D)
2033.10712033.0555253073260(K) (R)
2192.13772192.0334482342530(K) (S)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 27% (119/434AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 6/60 matches (10%).
Acc. #: Species: HUMAN Name: Gamma enolase (2-phospho-D-glycerate hydro-lyase) (Neural enolase) (NSE) (Enolase 2)
Index: MW: 47269 Da pI: 4.9
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1406.73331406.71671216280(R) (G)
1533.88561533.8463261061200(K) (A)
1570.81331570.7939122402530(K) (D)
1583.82401583.7753313593720(K) (S)
1804.98981804.94452533500(R) (D)
1938.99601938.98227.11631790(K) (D)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 20% (91/434AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 7/60 matches (11%).
Acc. #: Species: HUMAN Name: Alpha enolase, lung specific (2-phospho-D-glycerate hydro-lyase) (Non-neural enolase) (NNE) (Phosphopyruvate hydratase) (HLE1)
Index: MW: 49478 Da pI: 5.8
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1143.64221143.6162231932020(R) (N)
1425.76541425.7266272792900(R) (G)
1556.81901556.7783262492620(K) (D)
1572.82391572.7732321Met-ox 2492620(K)VVIGMDVAASEFYR(D)
1647.87671647.8376243653790(K) (L)
1809.89201809.836331961111(K) (F)
1938.99601939.0864-4778951(K) (I)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 18% (85/458AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 8/60 matches (13%).
Acc. #: Species: MOUSE Name: DNA topoisomerase III beta-1
Index: MW: 96950 Da pI: 8.2
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1143.64221143.619620911001(K) (F)
1526.73171526.702819pyroGlu 3563680(R)QQANHPYWADSVK(Q)
1540.82251540.8634-262963080(K) (V)
1543.78821543.7294383563680(R) (Q)
1556.81901556.8583-251Met-ox 2963080(K)QRPLALNTVEMLR(V)
1809.89201809.87021254690(K) (Y)
1813.82021813.8438-131Met-ox 24401(R)GNMSSHKGLNGACSVHK(Y)
1954.01301953.9639253093270(R) (L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 10% (88/862AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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