MS-Fit Search Results


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Parameters

Database searched: SwissProt.01.22.2003
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): 40-60
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 121920
Molecular weight search (1000 - 100000 Da) selects 115277 entries.
Full pI range: 121920 entries.
Species search ( HUMAN MOUSE ) selects 14893 entries.
Combined molecular weight, pI and species searches select 13163 entries.
Pre searches select 13163 entries.

Data Set 1 Results

MS-Fit search selects 467 entries (results displayed for top 5 matches).

Results Summary

MOWSE
Score		#/60(%)
Masses
Matched 		%
Cov		%
TIC		Mean
Err
ppm		Data
Tol
ppm		MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
15.842e+01022 (36)55.036.76.0723.0 40423/7.6HUMANPhosphoserine aminotransferase (PSAT)
29.139e+0059 (15)24.015.016.122.6 66018/8.2HUMANKeratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
33.300e+0048 (13)18.013.39.9249.7 61988/5.1HUMANKeratin, type I cytoskeletal 9 (Cytokeratin 9) (K9) (CK 9)
42.356e+0047 (11)16.011.7-4.9039.5 60124/8.1MOUSE60-kDa SS-A/Ro ribonucleoprotein (60 kDa Ro protein) (60 kDa ribonucleoprotein Ro) (RoRNP)
548216 (10)15.010.011.738.6 40473/8.1MOUSEPhosphoserine aminotransferase (PSAT) (Endometrial progesterone-induced protein) (EPIP)
82407...x.xx.xx.xxxxx.x.x.xxxx.x....x..x................xx......x
48753.......x..........x...........x.x...x.x.....x.........xx....
104932..x...........x.x..........x............xxx.............x...
50851...x.....x........................xx..x.........x.....x.....
94110........x..xx.........xx.x..................................


Detailed Results

1. 22/60 matches (36%).
Acc. #: Species: HUMAN Name: Phosphoserine aminotransferase (PSAT)
Index: MW: 40423 Da pI: 7.6
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
933.4383933.4504-131Met-ox 3643700(K)FLEMHQL(-)
1008.52711008.5301-2.91Met-ox 2993061(R)SKMNIPFR(I)
1019.55861019.55265.92142220(R) (E)
1037.57691037.5784-1.51912000(K) (N)
1045.56931045.5505183633701(K) (-)
1096.58141096.57912.1pyroGlu 6160(R)QVVNFGPGPAK(L)
1113.60321113.6057-2.26160(R) (L)
1125.64151125.6421-0.531181270(K) (L)
1138.54771138.54542.02232310(R) (V)
1147.64111147.63971.31Met-ox 3243330(K)ALELNMLSLK(G)
1161.62351161.61566.962710(R) (V)
1185.67061185.65929.652610(K) (E)
1253.73181253.7370-4.21171271(K) (L)
1270.76971270.7483172012130(K) (D)
1276.77091276.76296.317270(K) (E)
1284.66271284.6734-8.434450(K) (S)
1300.66401300.6683-3.41Met-ox 34450(K)GVGISVLEMSHR(S)
1441.69961441.678615981100(R) (A)
1550.83761550.8066203433560(R) (L)
2271.29722271.28256.52012221(K) (E)
2283.21462283.1365341Met-ox 1701900(K)GAVLVCDMSSNFLSKPVDVSK(F)
2871.43542871.3623252752980(K) (S)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 55% (204/370AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 9/60 matches (15%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
Index: MW: 66018 Da pI: 8.2
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1033.51631033.5166-0.224844920(R) (M)
1179.61531179.6010123773860(K) (H)
1383.71451383.6909171861970(K) (V)
1475.78341475.7494232122230(K) (T)
1475.78341475.7858-1.62002110(R) (W)
1687.98591687.97079.066821(R) (G)
1716.89521716.8517254184320(K) (G)
1994.02961993.9772262242390(R) (R)
2300.23842300.1985173443641(R) (S)
2384.02952383.9524325195490(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 24% (155/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 8/60 matches (13%).
Acc. #: Species: HUMAN Name: Keratin, type I cytoskeletal 9 (Cytokeratin 9) (K9) (CK 9)
Index: MW: 61988 Da pI: 5.1
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
897.4029897.4140-122332390(R) (I)
1138.54771138.5931-402332411(R) (F)
1157.59911157.59156.62502600(R) (Q)
1307.69581307.6782132402491(R) (Q)
1791.78441791.7283314905120(R) (G)
1838.00941837.9660243743890(R) (K)
1851.97931851.9275283213351(K) (N)
2705.23922705.16172963940(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 18% (114/622AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 7/60 matches (11%).
Acc. #: Species: MOUSE Name: 60-kDa SS-A/Ro ribonucleoprotein (60 kDa Ro protein) (60 kDa ribonucleoprotein Ro) (RoRNP)
Index: MW: 60124 Da pI: 8.1
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
933.4383933.43187.02082140(K) (E)
1045.56931045.6080-371601700(K) (Y)
1550.83761550.8001243713840(R) (A)
1566.77601566.7950-121Met-ox 3713840(R)FLLAVDVSASMNQR(A)
1716.89521716.88496.03483620(K) (T)
2177.25452177.2658-5.21812001(K) (Y)
2300.23842300.2780-173193381(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 16% (87/538AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 6/60 matches (10%).
Acc. #: Species: MOUSE Name: Phosphoserine aminotransferase (PSAT) (Endometrial progesterone-induced protein) (EPIP)
Index: MW: 40473 Da pI: 8.1
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
1037.57691037.5784-1.51912000(K) (N)
1096.58141096.57912.1pyroGlu 6160(K)QVVNFGPGPAK(L)
1113.60321113.6057-2.26160(K) (L)
1270.76971270.7483172012130(K) (D)
1276.77091276.76296.317270(K) (Q)
1298.75201298.68914834450(R) (S)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 15% (57/370AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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