MS-Fit Search Results



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Parameters

Database searched: SwissProt.01.22.2003
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): 40-61
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 121920
Molecular weight search (1000 - 100000 Da) selects 115277 entries.
Full pI range: 121920 entries.
Species search ( HUMAN MOUSE ) selects 14893 entries.
Combined molecular weight, pI and species searches select 13163 entries.
Pre searches select 13163 entries.

Data Set 1 Results

MS-Fit search selects 512 entries (results displayed for top 5 matches).

Results Summary

MOWSE
Score
#/60(%)
Masses
Matched
%
Cov
%
TIC
Mean
Err
ppm
Data
Tol
ppm
MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
11.291e+00713 (21)39.021.711.125.6 54637/8.8HUMANFumarate hydratase, mitochondrial precursor (Fumarase)
25.546e+0049 (15)19.015.017.736.1 78011/8.7HUMANCytoplasmic tyrosine-protein kinase BMX (Bone marrow kinase BMX) (Epithelial and endothelial tyrosine kinase) (ETK) (NTK38)
34.645e+0046 (10)12.010.05.3653.5 62644/6.2HUMANPutative transmembrane protein NMB precursor (Transmembrane glycoprotein HGFIN)
42.866e+0046 (10)16.010.06.1030.9 66018/8.2HUMANKeratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
51.808e+0047 (11)19.011.7-4.2539.6 47025/6.7MOUSEBeta enolase (2-phospho-D-glycerate hydro-lyase) (Skeletal muscle enolase) (Enolase 3)
71324.x...xx....xx.........xx..........x....x......x..xx.......x.
36351.............x....xx....x....x................xxx..x........
19908.........x...............x.....x.......x.x.............x....
48753...x....x.x..........x.........x..........x.................
10462..x.......x..x.x.x.x......x.................................



Detailed Results

1. 13/60 matches (21%).
Acc. #: Species: HUMAN Name: Fumarate hydratase, mitochondrial precursor (Fumarase)
Index: MW: 54637 Da pI: 8.8
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
957.4632957.4794-1767740(K) (S)
1214.58761214.5945-5.752610(R) (V)
1269.67881269.67046.61731830(K) (S)
1490.71791490.71273.51021150(R) (I)
1510.77191510.76544.362741(K) (S)
1763.94941763.9292112692860(R) (I)
1767.89711767.88059.452661(R) (Y)
2141.12042141.0800191Met-ox 3513710(R)SGLGELILPENEPGSSIMPGK(V)
2291.19782291.1416252Met-ox 4484670(K)LMNESLMLVTALNPHIGYDK(A)
2467.27982467.2397164845040(K) (D)
2494.27812494.2248214224440(R) (I)
2546.34832546.2891232342560(R) (Y)
3286.68003286.5915271Met-ox 1321600(K)LNDHFPLVVWQTGSGTQTNMNVNEVISNR(A)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 39% (203/510AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 9/60 matches (15%).
Acc. #: Species: HUMAN Name: Cytoplasmic tyrosine-protein kinase BMX (Bone marrow kinase BMX) (Epithelial and endothelial tyrosine kinase) (ETK) (NTK38)
Index: MW: 78011 Da pI: 8.7
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1541.77701541.7456205475591(R) (Y)
1613.88601613.8539203994130(K) (R)
1647.83301647.8052173233370(R) (A)
1777.92171777.8920173733870(K) (L)
1956.00831955.9802142222371(K) (E)
2467.27982467.1815405525731(K) (F)
2480.23432480.2825-1966851(K) (D)
2483.28202483.1764431Met-ox 5525731(K)VSDFGMTRYVLDDQYVSSVGTK(F)
2568.27042568.25037.81Met-ox 1761991(R)AVPVLKMDAPSSSTTLAQYDNESK(K)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 19% (132/675AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 6/60 matches (10%).
Acc. #: Species: HUMAN Name: Putative transmembrane protein NMB precursor (Transmembrane glycoprotein HGFIN)
Index: MW: 62644 Da pI: 6.2
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1425.72091425.6610422462571(K) (D)
1791.90131791.86012325390(R) (E)
1994.01591994.0284-6.31160(-) (L)
2291.19782291.2069-4.01942140(R) (R)
2307.23082307.2019131Met-ox 1942140(R)VSVNTANVTLGPQLMEVTVYR(R)
2807.35602807.4548-351902141(R) (R)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 12% (68/560AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 6/60 matches (10%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
Index: MW: 66018 Da pI: 8.2
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1179.58761179.6010-113773860(K) (H)
1383.68371383.6909-5.21861970(K) (V)
1475.76751475.7494122122230(K) (T)
1475.76751475.7858-122002110(R) (W)
1716.87621716.8517144184320(K) (G)
1994.01591993.9772192242390(R) (R)
2384.01352383.9524265195490(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 16% (108/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 7/60 matches (11%).
Acc. #: Species: MOUSE Name: Beta enolase (2-phospho-D-glycerate hydro-lyase) (Skeletal muscle enolase) (Enolase 3)
Index: MW: 47025 Da pI: 6.7
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1045.54181045.5893-4581891(K) (V)
1475.76751475.7858-124134261(R) (K)
1541.77701541.76478.03593720(K) (S)
1556.79121556.77838.32402530(K) (N)
1572.78051572.77324.71Met-ox 2402530(K)VVIGMDVAASEFYR(N)
1647.83301647.8376-2.83443580(K) (L)
1804.96251804.944510.033500(R) (D)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 19% (84/434AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):


MS-Fit in ProteinProspector 4.0.4
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