MS-Fit Search Results

MS-Fit Search Results

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Parameters

Database searched: SwissProt.01.22.2003
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): 40-63
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 121920
Molecular weight search (1000 - 100000 Da) selects 115277 entries.
Full pI range: 121920 entries.
Species search ( HUMAN MOUSE ) selects 14893 entries.
Combined molecular weight, pI and species searches select 13163 entries.
Pre searches select 13163 entries.

Data Set 1 Results

MS-Fit search selects 539 entries (results displayed for top 5 matches).

Results Summary

	MOWSE Score	#/60(%) Masses Matched	% Cov	% TIC	Mean Err ppm	Data Tol ppm	MS-Digest Index #	Protein MW (Da)/pI	Accession #	Species	Protein Name
1	3.404e+005	13 (21)	26.0	21.7	6.09	29.5		51707/8.1		HUMAN	Citrate synthase, mitochondrial precursor
2	2.225e+004	6 (10)	20.0	10.0	5.22	20.3		48443/8.7		HUMAN	Ubiquinol-cytochrome C reductase complex core protein 2, mitochondrial precursor (Complex III subunit II)
3	1.813e+004	9 (15)	10.0	15.0	-5.32	59.2		94845/6.5		MOUSE	Vacuolar protein sorting 16 (mVPS16)
4	1.599e+004	6 (10)	23.0	10.0	11.9	24.2		44537/7.5		MOUSE	Phosphoglycerate kinase 1
5	1.286e+004	7 (11)	27.0	11.7	12.2	22.1		44728/8.3		HUMAN	Phosphoglycerate kinase 1 (Primer recognition protein 2) (PRP 2)

50317	.	.	.	.	.	.	x	x	x	x	.	x	.	x	.	x	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	x	.	x	.	.	x	.	x	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.
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52466	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	x	.	x	.	x	.	.	.	.	.	.	.	x	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	x	x
73225	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	x	.	x	.	x	.	.	.	.	.	.	.	.	.	.
102942	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	x	.	.	x	.	.	.	.	.	.	x	.	x	.	x	.	.	.	.	.	.	.	.	.	.

Detailed Results

1. 13/60 matches (21%).
Acc. #: Species: HUMAN Name: Citrate synthase, mitochondrial precursor
Index: MW: 51707 Da pI: 8.1

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
1079.5141	1079.5196	-5.1		95	103	0	(R) (L)
1114.5408	1114.5355	4.7	1Met-ox	367	375	0	(K)HLPNDPMFK(L)
1127.6611	1127.6577	3.0		441	450	0	(R) (S)
1167.6720	1167.6639	6.9		341	351	0	(R) (K)
1209.7083	1209.7207	-10		383	393	0	(K) (A)
1326.8009	1326.7898	8.4		429	440	0	(R) (A)
1338.6501	1338.6442	4.4		330	340	0	(R) (V)
1607.8560	1607.8294	17		328	340	1	(K) (V)
1762.8869	1762.8863	0.34		77	92	0	(K) (F)
1773.8332	1773.8338	-0.33	1Met-ox	58	73	0	(K)TVVGQITVDMMYGGMR(G)
1789.8139	1789.8287	-8.3	2Met-ox	58	73	0	(K)TVVGQITVDMMYGGMR(G)
1805.8422	1805.8236	10	3Met-ox	58	73	0	(K)TVVGQITVDMMYGGMR(G)
1857.0221	1856.9322	48		194	208	0	(K) (L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 26% (122/466AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 6/60 matches (10%).
Acc. #: Species: HUMAN Name: Ubiquinol-cytochrome C reductase complex core protein 2, mitochondrial precursor (Complex III subunit II)
Index: MW: 48443 Da pI: 8.7

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1251.6040 1251.6156 -9.2 1Met-ox 232 241 0 (K)QVAEQFLNMR(G)
1580.7760 1580.7531 14 184 196 0 (R) (I)
1665.8207 1665.8237 -1.8 71 84 0 (R) (L)
1929.0561 1929.0446 6.0 43 60 0 (K) (I)
2165.0809 2165.0416 18 200 217 0 (K) (M)
2350.2142 2350.2056 3.7 163 183 0 (K) (N)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 20% (94/453AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 9/60 matches (15%).
Acc. #: Species: MOUSE Name: Vacuolar protein sorting 16 (mVPS16)
Index: MW: 94845 Da pI: 6.5

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1198.6961 1198.7159 -17 447 456 0 (K) (L)
1342.7663 1342.7768 -7.8 545 556 1 (R) (R)
1358.7814 1358.7717 7.1 1Met-ox 545 556 1 (R)SGEQVPLLLKMK(R)
1464.7619 1464.7204 28 1Met-ox 267 277 1 (K)QMVWCSRPRSK(E)
1665.8207 1665.9038 -50 462 474 0 (R) (L)
1762.8869 1762.8045 47 413 426 0 (R) (V)
2350.2142 2350.2883 -32 691 711 1 (R) (A)
2429.1746 2429.2222 -20 413 432 1 (R) (D)
2445.2059 2445.2171 -4.6 1Met-ox 413 432 1 (R)FPPDSFVHMCQDLRVLNAIR(D)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 10% (87/839AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 6/60 matches (10%).
Acc. #: Species: MOUSE Name: Phosphoglycerate kinase 1
Index: MW: 44537 Da pI: 7.5

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1342.7663 1342.7191 35 31 41 1 (K) (A)
1634.8047 1634.7927 7.3 157 171 0 (K) (A)
1769.0054 1768.9961 5.3 200 216 0 (K) (V)
1982.9668 1982.9381 14 247 264 0 (K) (I)
2023.0529 2023.0388 7.0 280 297 1 (K) (T)
2105.0653 2105.0609 2.1 333 350 0 (K) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 23% (97/417AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 7/60 matches (11%).
Acc. #: Species: HUMAN Name: Phosphoglycerate kinase 1 (Primer recognition protein 2) (PRP 2)
Index: MW: 44728 Da pI: 8.3

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1342.7663 1342.7191 35 31 41 1 (K) (A)
1634.8047 1634.7927 7.3 157 171 0 (K) (A)
1769.0054 1768.9961 5.3 200 216 0 (K) (V)
1782.9787 1782.9536 14 107 123 0 (K) (F)
1982.9668 1982.9381 14 1Met-ox 247 264 0 (K)VLNNMEIGTSLFDEEGAK(I)
2023.0529 2023.0388 7.0 280 297 1 (K) (T)
2105.0653 2105.0609 2.1 333 350 0 (K) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 27% (114/418AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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