MS-Fit Search Results


.

Parameters

Database searched: SwissProt.01.22.2003
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): 40-64
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 121920
Molecular weight search (1000 - 100000 Da) selects 115277 entries.
Full pI range: 121920 entries.
Species search ( HUMAN MOUSE ) selects 14893 entries.
Combined molecular weight, pI and species searches select 13163 entries.
Pre searches select 13163 entries.

Data Set 1 Results

MS-Fit search selects 544 entries (results displayed for top 5 matches).

Results Summary

MOWSE
Score		#/71(%)
Masses
Matched 		%
Cov		%
TIC		Mean
Err
ppm		Data
Tol
ppm		MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
11.803e+01023 (32)61.032.414.012.9 46531/8.1HUMANArgininosuccinate synthase (Citrulline--aspartate ligase)
21.476e+00511 (15)30.015.514.913.1 46585/8.4MOUSEArgininosuccinate synthase (Citrulline--aspartate ligase)
394527 (9)13.09.9-3.8550.4 66018/8.2HUMANKeratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
465626 (8)11.08.50.44540.3 61964/6.0HUMANDihydropyrimidinase related protein-3 (DRP-3) (Unc-33-like phosphoprotein) (ULIP protein) (Collapsin response mediator protein 4) (CRMP-4)
549238 (11)14.011.3-8.8057.9 95914/6.9HUMANRas GTPase-activating protein 3 (GAP1(IP4BP)) (Ins P4-binding protein)
14778x.......xxxx..x.x.x.xx....x.....x.xx..x............x...x.....x..x.x.xxx
66283x.......x..x..x.x...xx............x.............................x...x.x
48753...x..........x....x..x..x...........x...........x.....................
42578..........x...............................x.x..........xx........x.....
2794x..x..x.......................xx.........x.......................x....x


Detailed Results

1. 23/71 matches (32%).
Acc. #: Species: HUMAN Name: Argininosuccinate synthase (Citrulline--aspartate ligase)
Index: MW: 46531 Da pI: 8.1
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
905.5390905.5209203563630(K) (E)
956.4448956.4300151471530(R) (F)
964.5402964.51043159660(K) (E)
971.5800971.5638171011081(R) (E)
972.4455972.4249211Met-ox 1471530(R)MPEFYNR(F)
999.4560999.4457101Met-ox 1581650(R)NDLMEYAK(Q)
1088.57721088.57402.92002090(K) (T)
1092.61691092.60531158661(K) (E)
1186.70861186.6948121661760(K) (N)
1212.59771212.5683241Met-ox 1561651(K)GRNDLMEYAK(Q)
1486.79961486.7793142162280(K) (K)
1569.81941569.7987131281400(R) (V)
1604.92321604.894718871000(R) (K)
1614.89251614.8743112162291(K) (G)
1659.02021658.9958153493631(K) (E)
2127.11152127.09746.62102281(K) (K)
2239.14302239.1181111221401(K) (V)
2370.14122370.12885.21Met-ox 2402600(K)DGTTHQTSLELFMYLNEVAGK(H)
2388.13342388.11238.83173350(K) (H)
2429.19882429.16651367860(R) (Y)
2658.29762658.26881126481(K) (K)
2720.26052720.23369.92Met-ox 1771990(K)NPWSMDENLMHISYEAGILENPK(N)
2850.43782850.3773211Met-ox 2803040(R)GIYETPAGTILYHAHLDIEAFTMDR(E)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 61% (254/412AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 11/71 matches (15%).
Acc. #: Species: MOUSE Name: Argininosuccinate synthase (Citrulline--aspartate ligase)
Index: MW: 46585 Da pI: 8.4
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
905.5390905.5209203563630(K) (E)
956.4448956.4300151471530(R) (F)
972.4455972.4249211Met-ox 1471530(R)MPEFYNR(F)
999.4560999.4457101Met-ox 1581650(R)NDLMEYAK(Q)
1088.57721088.57402.92002090(K) (T)
1186.70861186.6948121661760(K) (S)
1212.59771212.5683241Met-ox 1561651(K)GRNDLMEYAK(Q)
1604.92321604.894718871000(R) (R)
2388.13342388.11238.83173350(K) (H)
2658.29762658.26881126481(K) (K)
2850.43782850.3773211Met-ox 2803040(R)GIYETPAGTILYHAHLDIEAFTMDR(E)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 30% (127/412AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 7/71 matches (9%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
Index: MW: 66018 Da pI: 8.2
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
910.3718910.4158-482702760(K) (R)
999.4560999.4457102902980(K) (V)
999.4560999.4972-413873951(R) (I)
1179.61411179.6010113773860(K) (H)
1383.71001383.6909141861970(K) (V)
1475.76351475.74949.52122230(K) (T)
1475.76351475.7858-152002110(R) (W)
1638.89351638.8604201861991(K) (F)
1993.98721993.97725.02242390(R) (R)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 13% (89/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 6/71 matches (8%).
Acc. #: Species: HUMAN Name: Dihydropyrimidinase related protein-3 (DRP-3) (Unc-33-like phosphoprotein) (ULIP protein) (Collapsin response mediator protein 4) (CRMP-4)
Index: MW: 61964 Da pI: 6.0
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
971.5800971.5526281501570(K) (D)
1710.85741710.8597-1.34524670(K) (F)
1726.85651726.85461.11Met-ox 4524670(K)IMLEDGNLHVTQGAGR(F)
2239.14302239.1830-182392581(R) (S)
2255.21692255.1780171Met-ox 2392581(R)AITIASQTNCPLYVTKVMSK(S)
2404.12622404.1857-255325541(R) (R)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 11% (67/570AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 8/71 matches (11%).
Acc. #: Species: HUMAN Name: Ras GTPase-activating protein 3 (GAP1(IP4BP)) (Ins P4-binding protein)
Index: MW: 95914 Da pI: 6.9
m/z
Submitted		MH+
Matched		Delta
ppm		ModificationsStartEndMissed
Cleavages		Database
Sequence
905.5390905.4997433413470(R) (V)
910.3718910.4014-323793850(K) (L)
927.4783927.4688108188240(K) (Q)
1536.70391536.7667-411Met-ox 6426531(K)MKNMFQVIQPER(A)
1567.76001567.74609.03723851(R) (L)
1707.79781707.8740-451151(-) (I)
2404.12622404.1461-8.32853040(R) (D)
2404.12622404.1807-236827001(K) (A)
2850.43782850.41757.11091321(R) (L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 14% (118/834AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
© Copyright (1995-2002) The Regents of the University of California.