MS-Fit Search Results



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Parameters

Database searched: SwissProt.01.22.2003
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): 40-65
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 121920
Molecular weight search (1000 - 100000 Da) selects 115277 entries.
Full pI range: 121920 entries.
Species search ( HUMAN MOUSE ) selects 14893 entries.
Combined molecular weight, pI and species searches select 13163 entries.
Pre searches select 13163 entries.

Data Set 1 Results

MS-Fit search selects 115 entries (results displayed for top 5 matches).

Results Summary

MOWSE
Score
#/38(%)
Masses
Matched
%
Cov
%
TIC
Mean
Err
ppm
Data
Tol
ppm
MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
13.411e+00613 (34)41.034.243.416.7 44537/7.5MOUSEPhosphoglycerate kinase 1
22.744e+00615 (39)49.039.542.616.1 44728/8.3HUMANPhosphoglycerate kinase 1 (Primer recognition protein 2) (PRP 2)
34466 (15)9.015.85.8560.9 86646/6.6MOUSESerine/threonine protein phosphatase with EF-hands-2 (PPEF-2)
44214 (10)24.010.54.8267.4 27866/8.8MOUSEProteasome subunit alpha type 7-like
53784 (10)10.010.518.979.0 58256/8.2HUMANCytochrome P450 7B1 (Oxysterol 7-alpha-hydroxylase)
73225x....xxx.x........x.x.x.......xx.x.xx.
102942x....xxx.x........x.x.x.x.....xx.xxxx.
57479..x...x................x.........x.xx.
13470...x.....x.....................xx.....
47543.....x...x..............x.........x...



Detailed Results

1. 13/38 matches (34%).
Acc. #: Species: MOUSE Name: Phosphoglycerate kinase 1
Index: MW: 44537 Da pI: 7.5
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
884.4950884.4518491931990(K) (A)
1101.59641101.54015131390(K) (I)
1159.62271159.5748411241331(R) (D)
1383.76531383.7055431Met-ox 1721840(R)AHSSMVGVNLPQK(A)
1634.85991634.7927411571710(K) (A)
1740.99211740.9132453894060(K) (V)
1759.96161759.8763482Met-ox 2312460(K)VNEMIIGGGMAFTFLK(V)
1769.06961768.9961422002160(K) (V)
1983.03261982.9381482472640(K) (I)
2023.13382023.0388472802971(K) (T)
2048.10612047.9983531Met-ox 23391(R)VDFNVPMKNNQITNNQR(I)
2105.13522105.0609353333500(K) (G)
2121.11642121.0729211521711(R) (A)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 41% (172/417AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 15/38 matches (39%).
Acc. #: Species: HUMAN Name: Phosphoglycerate kinase 1 (Primer recognition protein 2) (PRP 2)
Index: MW: 44728 Da pI: 8.3
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
884.4950884.4518491931990(K) (A)
1101.59641101.54015131390(K) (I)
1159.62271159.5748411241331(R) (D)
1383.76531383.7055431Met-ox 1721840(R)AHSSMVGVNLPQK(A)
1634.85991634.7927411571710(K) (A)
1740.99211740.9132453894060(K) (V)
1759.96161759.8763482Met-ox 2312460(K)VNEMIIGGGMAFTFLK(V)
1769.06961768.9961422002160(K) (V)
1783.02481782.9536401071230(K) (F)
1983.03261982.9381481Met-ox 2472640(K)VLNNMEIGTSLFDEEGAK(I)
2023.13382023.0388472802971(K) (T)
2048.10612047.9983531Met-ox 23391(R)VDFNVPMKNNQITNNQR(I)
2067.11002067.0367352Met-ox 57750(K)SVVLMSHLGRPDGVPMPDK(Y)
2105.13522105.0609353333500(K) (G)
2121.11642121.0729211521711(R) (A)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 49% (208/418AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 6/38 matches (15%).
Acc. #: Species: MOUSE Name: Serine/threonine protein phosphatase with EF-hands-2 (PPEF-2)
Index: MW: 86646 Da pI: 6.6
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
972.4827972.5267-45pyroGlu 4054130(R)QQAGFLGIR(E)
1159.62271159.6435-185555641(R) (A)
1773.97731773.9347246396531(K) (L)
2048.10612048.0453305065231(R) (G)
2105.13522105.0813266136300(R) (S)
2121.11642121.0763191Met-ox 6136300(R)MLRPQLVNSSADNVLEYR(S)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 9% (70/757AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 4/38 matches (10%).
Acc. #: Species: MOUSE Name: Proteasome subunit alpha type 7-like
Index: MW: 27866 Da pI: 8.8
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1045.61831045.56425251591(K) (T)
1634.85991634.8641-2.6981110(K) (F)
2023.13382023.1916-291962141(K) (R)
2045.07942045.0820-1.31271450(R) (L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 24% (61/250AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 4/38 matches (10%).
Acc. #: Species: HUMAN Name: Cytochrome P450 7B1 (Oxysterol 7-alpha-hydroxylase)
Index: MW: 58256 Da pI: 8.2
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1101.59641101.6308-311631710(K) (T)
1634.85991634.84976.21491620(K) (Q)
1783.02481782.9430464875010(R) (Y)
2067.11002066.9969552572731(K) (Y)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 10% (55/506AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):


MS-Fit in ProteinProspector 4.0.4
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