MS-Fit Search Results



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Parameters

Database searched: SwissProt.01.22.2003
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): 40-66
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 121920
Molecular weight search (1000 - 100000 Da) selects 115277 entries.
Full pI range: 121920 entries.
Species search ( HUMAN MOUSE ) selects 14893 entries.
Combined molecular weight, pI and species searches select 13163 entries.
Pre searches select 13163 entries.

Data Set 1 Results

MS-Fit search selects 700 entries (results displayed for top 5 matches).

Results Summary

MOWSE
Score
#/76(%)
Masses
Matched
%
Cov
%
TIC
Mean
Err
ppm
Data
Tol
ppm
MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
12.549e+00717 (22)52.022.416.147.2 42040/8.5HUMAN3-ketoacyl-CoA thiolase, mitochondrial (Beta-ketothiolase) (Acetyl-CoA acyltransferase) (Mitochondrial 3-oxoacyl-CoA thiolase) (T1)
23.664e+00614 (18)36.018.44.5140.4 44730/7.6MOUSEPutative GTP-binding protein PTD004 homolog
33.663e+00614 (18)36.018.44.5140.4 44744/7.6HUMANPutative GTP-binding protein PTD004 (PRO2455)
46.348e+0047 (9)22.09.27.7038.3 44537/7.5MOUSEPhosphoglycerate kinase 1
51.212e+0046 (7)10.07.9-10.246.1 56558/5.7HUMANSestrin 1 (p53-regulated protein PA26)
88213.....x....x....x...x.x..x....x.............x.....x....xx...........x.x.xx.xx
110209......x.......x........x....x...x.x.x...x...x.....xx.x.......x.x............
62777......x.......x........x....x...x.x.x...x...x.....xx.x.......x.x............
73225...............x.....................xx.......x.........x...x.x.............
112987..............x........x...............x..............xx..x.................



Detailed Results

1. 17/76 matches (22%).
Acc. #: Species: HUMAN Name: 3-ketoacyl-CoA thiolase, mitochondrial (Beta-ketothiolase) (Acetyl-CoA acyltransferase) (Mitochondrial 3-oxoacyl-CoA thiolase) (T1)
Index: MW: 42040 Da pI: 8.5
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
955.4784955.5114-352712780(K) (I)
993.5217993.511810.01821890(K) (W)
1083.58301083.6063-222702781(K) (I)
1123.59931123.6152-1415250(R) (D)
1188.67701188.6853-7.03613700(R) (R)
1279.72401279.71636.014251(K) (D)
1415.68121415.66958.326380(K) (A)
1668.81271668.7652281771891(R) (W)
1835.00821834.9524303413600(K) (I)
1954.92261954.8857191922090(K) (T)
1970.93001970.8806251Met-ox 1922090(K)AANDAGYFNDEMAPIEVK(T)
2325.19642325.1073381Met-ox 3133320(K)DMDLVEVNEAFAPQYLAVER(S)
2380.26572380.156746pyroGlu 1Met-ox 2152340(K)QTMQVDEHARPQTTLEQLQK(L)
2387.13392387.0681281Met-ox 1101300(K)EAEVVLCGGTESMSQAPYCVR(N)
2397.27702397.1832391Met-ox 2152340(K)QTMQVDEHARPQTTLEQLQK(L)
2516.33412516.2480342422690(K) (K)
2767.48772767.3824381Met-ox 46710(K)VSPETVDSVIMGNVLQSSSDAIYLAR(H)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 52% (210/397AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 14/76 matches (18%).
Acc. #: Species: MOUSE Name: Putative GTP-binding protein PTD004 homolog
Index: MW: 44730 Da pI: 7.6
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
959.4599959.4951-373873940(K) (K)
1074.50741074.5431-332712790(K) (Q)
1276.72921276.7377-6.76180(K) (F)
1404.84141404.83276.25181(K) (F)
1481.72531481.7310-3.81Met-ox 2822940(K)YLEANMTQSALPK(I)
1568.96521568.94161584980(K) (G)
1572.83441572.7950253743860(R) (F)
1654.84261654.80522371830(R) (I)
1707.81311707.81260.273413541(K) (D)
1835.99551835.99410.791Met-ox 2822971(K)YLEANMTQSALPKIIK(A)
1910.09441910.08644.26241(K) (I)
1949.99551949.9398292022161(R) (H)
2014.97402014.9358191261430(R) (D)
2025.05252025.008222pyroGlu 3703861(R)QQGRNYIVEDGDIIFFK(F)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 36% (145/396AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 14/76 matches (18%).
Acc. #: Species: HUMAN Name: Putative GTP-binding protein PTD004 (PRO2455)
Index: MW: 44744 Da pI: 7.6
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
959.4599959.4951-373873940(K) (K)
1074.50741074.5431-332712790(K) (Q)
1276.72921276.7377-6.76180(K) (F)
1404.84141404.83276.25181(K) (F)
1481.72531481.7310-3.81Met-ox 2822940(K)YLEANMTQSALPK(I)
1568.96521568.94161584980(K) (G)
1572.83441572.7950253743860(R) (F)
1654.84261654.80522371830(R) (I)
1707.81311707.81260.273413541(K) (E)
1835.99551835.99410.791Met-ox 2822971(K)YLEANMTQSALPKIIK(A)
1910.09441910.08644.26241(K) (I)
1949.99551949.9398292022161(R) (H)
2014.97402014.9358191261430(R) (D)
2025.05252025.008222pyroGlu 3703861(R)QQGRNYIVEDGDIIFFK(F)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 36% (145/396AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 7/76 matches (9%).
Acc. #: Species: MOUSE Name: Phosphoglycerate kinase 1
Index: MW: 44537 Da pI: 7.5
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1083.58301083.6050-204074170(K) (-)
1614.87481614.8889-8.72172301(K) (V)
1634.78651634.7927-3.81571710(K) (A)
1769.02561768.9961172002160(K) (V)
1982.99531982.9381292472640(K) (I)
1998.99191998.9330291Met-ox 2472640(K)VLNNMEIGTSLYDEEGAK(I)
2023.06252023.0388122802971(K) (T)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 22% (93/417AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 6/76 matches (7%).
Acc. #: Species: HUMAN Name: Sestrin 1 (p53-regulated protein PA26)
Index: MW: 56558 Da pI: 5.7
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1074.50741074.50670.5931390(R) (I)
1276.72921276.7741-354654750(K) (M)
1648.85251648.9209-414764891(R) (Y)
1954.92261954.9308-4.23793940(K) (D)
1970.93001970.92572.21Met-ox 3793940(K)FHIAYNLTYNTMAMHK(D)
1986.95481986.9206172Met-ox 3793940(K)FHIAYNLTYNTMAMHK(D)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 10% (50/492AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):


MS-Fit in ProteinProspector 4.0.4
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