MS-Fit Search Results

MS-Fit Search Results

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Parameters

Database searched: SwissProt.01.22.2003
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): 40-67
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 121920
Molecular weight search (1000 - 100000 Da) selects 115277 entries.
Full pI range: 121920 entries.
Species search ( HUMAN MOUSE ) selects 14893 entries.
Combined molecular weight, pI and species searches select 13163 entries.
Pre searches select 13163 entries.

Data Set 1 Results

MS-Fit search selects 574 entries (results displayed for top 5 matches).

Results Summary

	MOWSE Score	#/60(%) Masses Matched	% Cov	% TIC	Mean Err ppm	Data Tol ppm	MS-Digest Index #	Protein MW (Da)/pI	Accession #	Species	Protein Name
1	1.260e+006	12 (20)	43.0	20.0	-19.6	20.5		39420/8.3		HUMAN	Fructose-bisphosphate aldolase A (Muscle-type aldolase) (Lung cancer antigen NY-LU-1)
2	1.687e+005	8 (13)	22.0	13.3	-3.50	67.5		48357/6.4		HUMAN	Interferon consensus sequence binding protein (ICSBP)
3	9.588e+004	8 (13)	30.0	13.3	-23.0	17.2		39356/8.3		MOUSE	Fructose-bisphosphate aldolase A (Muscle-type aldolase)
4	8378	7 (11)	19.0	11.7	-8.68	61.0		53083/7.6		HUMAN	Serine hydroxymethyltransferase, cytosolic (Serine methylase) (Glycine hydroxymethyltransferase) (SHMT)
5	7677	10 (16)	19.0	16.7	-7.01	38.4		82861/9.3		HUMAN	Plakophilin 1 (Band-6-protein) (B6P)

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Detailed Results

1. 12/60 matches (20%).
Acc. #: Species: HUMAN Name: Fructose-bisphosphate aldolase A (Muscle-type aldolase) (Lung cancer antigen NY-LU-1)
Index: MW: 39420 Da pI: 8.3

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
1027.4983	1027.5424	-43	pyroGlu	61	69	0	(R)QLLLTADDR(V)
1044.5403	1044.5689	-27		61	69	0	(R) (V)
1093.5424	1093.5642	-20		323	331	1	(K) (A)
1342.6847	1342.7119	-20		88	99	0	(K) (S)
1646.7806	1646.8098	-18		44	57	1	(R) (F)
1646.7806	1646.8098	-18		43	56	1	(K) (R)
1691.8198	1691.8427	-14	1Met-ox	244	258	0	(K)FSHEEIAMATVTALR(R)
1822.9182	1822.9678	-27		290	304	0	(K) (A)
1847.9116	1847.9438	-17	1Met-ox	244	259	1	(K)FSHEEIAMATVTALRR(T)
2102.0658	2102.1109	-21		70	87	0	(R) (A)
2123.0570	2123.0919	-16	1Met-ox	154	173	0	(K)IGEHTPSALAIMENANVLAR(Y)
2228.0330	2228.0260	3.2		343	364	0	(K) (-)
2272.1052	2272.1421	-16		112	134	0	(K) (C)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 43% (159/364AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 8/60 matches (13%).
Acc. #: Species: HUMAN Name: Interferon consensus sequence binding protein (ICSBP)
Index: MW: 48357 Da pI: 6.4

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
868.5187 868.5005 21 277 284 0 (R) (Q)
1179.5731 1179.5897 -14 99 108 0 (R) (V)
1199.6474 1199.6061 34 252 262 0 (R) (Q)
1701.9478 1702.0267 -46 362 375 1 (R) (Q)
1893.8443 1893.9243 -42 346 361 0 (R) (S)
1909.8585 1909.9192 -32 1Met-ox 346 361 0 (R)VVLCFGEEFPDMAPLR(S)
1949.9833 1949.9357 24 42 58 1 (K) (A)
2273.0935 2273.0331 27 384 404 0 (K) (M)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 22% (97/426AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 8/60 matches (13%).
Acc. #: Species: MOUSE Name: Fructose-bisphosphate aldolase A (Muscle-type aldolase)
Index: MW: 39356 Da pI: 8.3

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1027.4983 1027.5424 -43 pyroGlu 61 69 0 (R)QLLLTADDR(V)
1044.5403 1044.5689 -27 61 69 0 (R) (V)
1342.6847 1342.7119 -20 88 99 0 (K) (S)
1646.7806 1646.8098 -18 44 57 1 (R) (F)
1646.7806 1646.8098 -18 43 56 1 (K) (R)
1822.9182 1822.9678 -27 290 304 0 (K) (A)
2102.0658 2102.1109 -21 70 87 0 (R) (A)
2123.0570 2123.0919 -16 1Met-ox 154 173 0 (K)IGEHTPSALAIMENANVLAR(Y)
2272.1052 2272.1421 -16 112 134 0 (K) (C)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 30% (112/364AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 7/60 matches (11%).
Acc. #: Species: HUMAN Name: Serine hydroxymethyltransferase, cytosolic (Serine methylase) (Glycine hydroxymethyltransferase) (SHMT)
Index: MW: 53083 Da pI: 7.6

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1019.5296 1019.5638 -34 457 465 0 (K) (E)
1402.7599 1402.7364 17 60 72 0 (R) (Y)
1505.7311 1505.7964 -43 46 59 0 (R) (A)
1993.9211 1993.9799 -30 466 483 0 (R) (-)
2272.1052 2272.0127 41 2Met-ox 1 20 1 (-)MTMPVNGAHKDADLWSSHDK(M)
2283.0894 2283.1266 -16 138 158 1 (R) (K)
2299.1317 2299.1215 4.4 1Met-ox 138 158 1 (R)IMGLDLPDGGHLTHGFMTDKK(K)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 19% (95/483AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 10/60 matches (16%).
Acc. #: Species: HUMAN Name: Plakophilin 1 (Band-6-protein) (B6P)
Index: MW: 82861 Da pI: 9.3

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1179.5731 1179.6084 -30 706 715 0 (R) (E)
1219.6841 1219.6435 33 486 495 1 (R) (Q)
1475.7066 1475.7350 -19 pyroGlu 1Met-ox 39 50 1 (R)QRVQEQVMMTVK(R)
1505.7311 1505.7308 0.16 54 67 1 (K) (G)
1505.7311 1505.7494 -12 432 445 1 (R) (N)
1707.7740 1707.7648 5.4 503 517 1 (R) (S)
1847.9116 1847.9173 -3.1 562 579 0 (K) (G)
1909.8585 1909.8723 -7.2 471 485 0 (K) (L)
1922.9247 1922.9871 -32 729 746 1 (R) (F)
2123.0570 2123.0364 9.7 231 249 1 (R) (A)
2807.2673 2807.3279 -22 1Met-ox 471 493 1 (K)SVENCMCVLHNLSYRLDAEVPTR(Y)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 19% (145/747AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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