MS-Fit Search Results



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Parameters

Database searched: SwissProt.01.22.2003
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): 40-69
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 121920
Molecular weight search (1000 - 100000 Da) selects 115277 entries.
Full pI range: 121920 entries.
Species search ( HUMAN MOUSE ) selects 14893 entries.
Combined molecular weight, pI and species searches select 13163 entries.
Pre searches select 13163 entries.

Data Set 1 Results

MS-Fit search selects 614 entries (results displayed for top 5 matches).

Results Summary

MOWSE
Score
#/60(%)
Masses
Matched
%
Cov
%
TIC
Mean
Err
ppm
Data
Tol
ppm
MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
18.938e+01019 (31)69.031.7-7.3915.3 39420/8.3HUMANFructose-bisphosphate aldolase A (Muscle-type aldolase) (Lung cancer antigen NY-LU-1)
22.697e+00711 (18)40.018.3-7.2714.9 39356/8.3MOUSEFructose-bisphosphate aldolase A (Muscle-type aldolase)
31.266e+0049 (15)13.015.0-3.9135.9 98106/8.7HUMANMitotic kinesin-like protein-1 (Kinesin-like protein 5)
464005 (8)26.08.33.7073.4 29022/8.4MOUSEGLANDULAR KALLIKREIN K6 PRECURSOR (TISSUE KALLIKREIN) (MGK-1)
542544 (6)9.06.722.333.5 71277/8.7MOUSELong-chain fatty acid transport protein precursor (FATP)
49811.xx.xx.....x............xxx........xx.x.....xxx..x.x...x.xx.
41562.xx.x......x............x..........x........xxx....x......x.
65336...............x.........xx.xx.x....x.........x....x........
45468....................x....xx.................x.........x.....
83423..................................x..........xx.......x.....



Detailed Results

1. 19/60 matches (31%).
Acc. #: Species: HUMAN Name: Fructose-bisphosphate aldolase A (Muscle-type aldolase) (Lung cancer antigen NY-LU-1)
Index: MW: 39420 Da pI: 8.3
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
940.4656940.4852-2115220(K) (I)
1027.52381027.5424-18pyroGlu 61690(R)QLLLTADDR(V)
1044.55021044.5689-1861690(R) (V)
1093.54811093.5642-153233311(K) (A)
1342.70571342.7119-4.688990(K) (S)
1646.80841646.8098-0.8244571(R) (F)
1646.80841646.8098-0.8243561(K) (R)
1675.81911675.8478-172442580(K) (R)
1691.82861691.8427-8.31Met-ox 2442580(K)FSHEEIAMATVTALR(R)
1822.95481822.9678-7.12903040(K) (A)
1831.92531831.9489-132442591(K) (T)
1847.93751847.9438-3.41Met-ox 2442591(K)FSHEEIAMATVTALRR(T)
2102.10682102.1109-1.970870(R) (A)
2107.08592107.0970-5.31541730(K) (Y)
2123.09212123.09190.0891Met-ox 1541730(K)IGEHTPSALAIMENANVLAR(Y)
2228.04152228.02607.03433640(K) (-)
2272.12692272.1421-6.71121340(K) (C)
3057.53693057.5744-122602890(R) (C)
3161.59103161.58761.11Met-ox 2162430(K)ALSDHHIYLEGTLLKPNMVTPGHACTQK(F)
3190.61093190.6207-3.11742011(R) (C)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 69% (253/364AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 11/60 matches (18%).
Acc. #: Species: MOUSE Name: Fructose-bisphosphate aldolase A (Muscle-type aldolase)
Index: MW: 39356 Da pI: 8.3
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
940.4656940.4852-2115220(K) (I)
1027.52381027.5424-18pyroGlu 61690(R)QLLLTADDR(V)
1044.55021044.5689-1861690(R) (V)
1342.70571342.7119-4.688990(K) (S)
1646.80841646.8098-0.8244571(R) (F)
1646.80841646.8098-0.8243561(K) (R)
1822.95481822.9678-7.12903040(K) (A)
2102.10682102.1109-1.970870(R) (A)
2107.08592107.0970-5.31541730(K) (Y)
2123.09212123.09190.0891Met-ox 1541730(K)IGEHTPSALAIMENANVLAR(Y)
2272.12692272.1421-6.71121340(K) (C)
3190.61093190.6207-3.11742011(R) (C)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 40% (148/364AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 9/60 matches (15%).
Acc. #: Species: HUMAN Name: Mitotic kinesin-like protein-1 (Kinesin-like protein 5)
Index: MW: 98106 Da pI: 8.7
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1446.70351446.7375-233563680(R) (T)
1675.81911675.80498.55055171(R) (Q)
1675.81911675.8305-6.88378520(R) (R)
1691.82861691.7998171Met-ox 5055171(R)HNLRQMMIDEFNK(Q)
1707.81551707.7947122Met-ox 5055171(R)HNLRQMMIDEFNK(Q)
1707.81551707.8488-2017311(K) (V)
1707.81551707.8774-366106241(R) (W)
1715.91181715.9227-6.33543681(R) (T)
1764.85251764.8049272572710(K) (S)
1831.92531831.9316-3.48378531(R) (K)
2123.09212123.1137-105185361(K) (E)
2272.12692272.1396-5.65245431(K) (L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 13% (116/856AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 5/60 matches (8%).
Acc. #: Species: MOUSE Name: GLANDULAR KALLIKREIN K6 PRECURSOR (TISSUE KALLIKREIN) (MGK-1)
Index: MW: 29022 Da pI: 8.4
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1505.74331505.7820-261922061(K) (D)
1675.81911675.783721951070(K) (N)
1691.82861691.7786301Met-ox 951070(K)SFLHPCYNMSLHR(N)
2102.10682102.0262381701861(K) (A)
2615.32532615.4424-451240(-) (I)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 26% (69/261AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 4/60 matches (6%).
Acc. #: Species: MOUSE Name: Long-chain fatty acid transport protein precursor (FATP)
Index: MW: 71277 Da pI: 8.7
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1802.91871802.8570343293420(K) (Y)
2107.08592107.07455.41781980(K) (S)
2123.09212123.0694111Met-ox 1781980(K)ALIYGGEMAAAVAEVSEQLGK(S)
2615.32532615.2234395515740(K) (V)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 9% (59/646AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):


MS-Fit in ProteinProspector 4.0.4
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