MS-Fit Search Results

Parameters

Database searched: SwissProt.10.25.2002
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): 40-70
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 116819
Molecular weight search (1000 - 100000 Da) selects 110413 entries.
Full pI range: 116819 entries.
Species search ( HUMAN MOUSE ) selects 14257 entries.
Combined molecular weight, pI and species searches select 12612 entries.
Pre searches select 12612 entries.

Data Set 1 Results

MS-Fit search selects 2373 entries (results displayed for top 5 matches).

Results Summary

	MOWSE Score	#/97(%) Masses Matched	% Cov	% TIC	Mean Err ppm	Data Tol ppm	Protein MW (Da)/pI	Species	Protein Name
1	1.420e+010	22 (22)	54.0	22.7	23.3	58.3	47476/9.1	HUMAN	Aspartate aminotransferase, mitochondrial precursor (Transaminase A) (Glutamate oxaloacetate transaminase-2)
2	2.194e+006	17 (17)	45.0	17.5	25.5	54.9	47412/9.1	MOUSE	ASPARTATE AMINOTRANSFERASE, MITOCHONDRIAL PRECURSOR (TRANSAMINASE A) (GLUTAMATE OXALOACETATE TRANSAMINASE-2)
3	1.222e+006	13 (13)	18.0	13.4	12.6	58.3	73244/6.7	HUMAN	DEAD-box protein 3 (Helicase-like protein 2) (HLP2) (DEAD-box, X isoform)
4	1.170e+006	12 (12)	19.0	12.4	3.72	63.3	73095/7.0	HUMAN	DEAD-box protein 3, Y-chromosomal
5	1.163e+006	12 (12)	16.0	12.4	11.7	71.1	73141/6.7	MOUSE	Putative ATP-dependent RNA helicase PL10

82072	x	.	x	.	x	x	.	.	.	x	.	.	.	.	.	x	.	.	.	x	.	.	.	.	x	.	x	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	x	x	.	x	.	x	.	.	.	.	.	.	x	x	.	.	.	.	.	.	.	.	.	.	.	.	x	.	x	.	.	.	.	.	.	.	.	x	.	.	.	x	.	x
53127	.	.	x	.	x	x	.	.	.	x	.	.	.	.	.	x	.	.	.	x	.	.	.	.	x	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	x	x	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	x	.	.	.	x	.	.	.	.	x	.	.	.	.	.	x
3478	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	x	.	x	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	x	x	.	x	.	.	x	x	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.
27789	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	x	.	.	.	.	.	.	.	.	x	x	.	x	.	.	x	x	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	x	.	.	x	.	.	.	.	.	.	.
101231	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	x	x	.	.	.	.	.	.	.	.	.	x	x	.	x	.	.	x	x	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.

Detailed Results

1. 22/97 matches (22%).
Acc. #: Species: HUMAN Name: Aspartate aminotransferase, mitochondrial precursor (Transaminase A) (Glutamate oxaloacetate transaminase-2)
Index: MW: 47476 Da pI: 9.1

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
891.5378	891.5052	36		140	147	0	(R) (F)
902.4835	902.5311	-53		356	363	0	(R) (K)
913.5210	913.4783	47	pyroGlu	339	345	0	(K)QWLQEVK(G)
930.5281	930.5049	25		339	345	0	(K) (G)
955.4564	955.4307	27	1Met-ox	280	287	0	(K)NMGLYGER(V)
996.5136	996.4937	20	1Met-ox	60	68	0	(K)MNLGVGAYR(D)
1058.5716	1058.5999	-27		338	345	1	(R) (G)
1124.5948	1124.5886	5.5	1Met-ox	59	68	1	(K)KMNLGVGAYR(D)
1154.5685	1154.5264	36	1Met-ox	171	180	0	(R)DAGMQLQGYR(Y)
1267.7895	1267.7374	41		326	337	0	(R) (K)
1449.8660	1449.8066	41		126	139	0	(R) (I)
1459.8111	1459.7545	39		69	81	0	(R) (K)
1460.8042	1460.7320	49		339	350	1	(K) (I)
1530.8428	1530.8015	27		108	122	0	(K) (S)
1660.8303	1660.7529	47		186	200	0	(K) (I)
1811.9062	1811.9954	-49		310	325	0	(K) (I)
1828.0525	1827.9903	34	1Met-ox	310	325	0	(K)ILIRPMYSNPPLNGAR(I)
1962.9704	1962.9240	24	1Met-ox	280	296	1	(K)NMGLYGERVGAFTMVCK(D)
1981.0694	1981.0105	30		91	107	1	(K) (A)
2095.1026	2095.0105	44		263	279	0	(R) (N)
2352.3685	2352.2676	43		408	430	0	(R) (-)
3106.6695	3106.5896	26		201	227	0	(K) (E)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 54% (235/430AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 17/97 matches (17%).
Acc. #: Species: MOUSE Name: ASPARTATE AMINOTRANSFERASE, MITOCHONDRIAL PRECURSOR (TRANSAMINASE A) (GLUTAMATE OXALOACETATE TRANSAMINASE-2)
Index: MW: 47412 Da pI: 9.1

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
902.4835 902.5311 -53 356 363 0 (R) (K)
913.5210 913.4783 47 pyroGlu 339 345 0 (K)QWLQEVK(G)
930.5281 930.4831 48 1Met-ox 1 8 0 (-)MALLHSSR(I)
930.5281 930.5049 25 339 345 0 (K) (G)
955.4564 955.4307 27 1Met-ox 280 287 0 (K)NMGLYGER(V)
996.5136 996.4937 20 1Met-ox 60 68 0 (K)MNLGVGAYR(D)
1058.5716 1058.5999 -27 338 345 1 (R) (G)
1124.5948 1124.5886 5.5 1Met-ox 59 68 1 (K)KMNLGVGAYR(D)
1154.5685 1154.5264 36 1Met-ox 171 180 0 (R)DAGMQLQGYR(Y)
1449.8660 1449.8066 41 126 139 0 (R) (V)
1459.8111 1459.7545 39 69 81 0 (R) (K)
1460.8042 1460.7320 49 339 350 1 (K) (I)
1660.8303 1660.7529 47 186 200 0 (K) (I)
1883.0123 1882.9962 8.6 9 28 0 (R) (A)
1981.0694 1981.0105 30 91 107 1 (K) (A)
2011.1456 2011.0554 45 154 170 0 (R) (D)
2095.1026 2095.0105 44 263 279 0 (R) (N)
3106.6695 3106.5896 26 201 227 0 (K) (E)

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
902.4835	902.5311	-53		356	363	0	(R) (K)
913.5210	913.4783	47	pyroGlu	339	345	0	(K)QWLQEVK(G)
930.5281	930.4831	48	1Met-ox	1	8	0	(-)MALLHSSR(I)
930.5281	930.5049	25		339	345	0	(K) (G)
955.4564	955.4307	27	1Met-ox	280	287	0	(K)NMGLYGER(V)
996.5136	996.4937	20	1Met-ox	60	68	0	(K)MNLGVGAYR(D)
1058.5716	1058.5999	-27		338	345	1	(R) (G)
1124.5948	1124.5886	5.5	1Met-ox	59	68	1	(K)KMNLGVGAYR(D)
1154.5685	1154.5264	36	1Met-ox	171	180	0	(R)DAGMQLQGYR(Y)
1449.8660	1449.8066	41		126	139	0	(R) (V)
1459.8111	1459.7545	39		69	81	0	(R) (K)
1460.8042	1460.7320	49		339	350	1	(K) (I)
1660.8303	1660.7529	47		186	200	0	(K) (I)
1883.0123	1882.9962	8.6		9	28	0	(R) (A)
1981.0694	1981.0105	30		91	107	1	(K) (A)
2011.1456	2011.0554	45		154	170	0	(R) (D)
2095.1026	2095.0105	44		263	279	0	(R) (N)
3106.6695	3106.5896	26		201	227	0	(K) (E)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 45% (197/430AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 13/97 matches (13%).
Acc. #: Species: HUMAN Name: DEAD-box protein 3 (Helicase-like protein 2) (HLP2) (DEAD-box, X isoform)
Index: MW: 73244 Da pI: 6.7

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1030.4941 1030.4958 -1.6 80 88 1 (R) (G)
1058.5716 1058.5159 53 47 55 1 (R) (D)
1090.5545 1090.5421 11 419 427 0 (K) (R)
1157.5739 1157.5876 -12 364 373 0 (R) (G)
1329.7389 1329.6837 42 1Met-ox 363 373 1 (R)RIVEQDTMPPK(G)
1336.6384 1336.6394 -0.68 352 362 0 (R) (R)
1340.7499 1340.7578 -5.9 pyroGlu 265 276 0 (K)QYPISLVLAPTR(E)
1357.7228 1357.7844 -45 265 276 0 (K) (E)
1368.6148 1368.6292 -10 2Met-ox 352 362 0 (R)MLDMGFEPQIR(R)
1448.8257 1448.7749 35 277 288 1 (R) (F)
1883.0123 1882.9187 50 146 162 0 (R) (Y)
1941.0006 1940.9792 11 336 351 1 (K) (M)
2083.1040 2083.0249 38 512 528 0 (K) (I)

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
1030.4941	1030.4958	-1.6		80	88	1	(R) (G)
1058.5716	1058.5159	53		47	55	1	(R) (D)
1090.5545	1090.5421	11		419	427	0	(K) (R)
1157.5739	1157.5876	-12		364	373	0	(R) (G)
1329.7389	1329.6837	42	1Met-ox	363	373	1	(R)RIVEQDTMPPK(G)
1336.6384	1336.6394	-0.68		352	362	0	(R) (R)
1340.7499	1340.7578	-5.9	pyroGlu	265	276	0	(K)QYPISLVLAPTR(E)
1357.7228	1357.7844	-45		265	276	0	(K) (E)
1368.6148	1368.6292	-10	2Met-ox	352	362	0	(R)MLDMGFEPQIR(R)
1448.8257	1448.7749	35		277	288	1	(R) (F)
1883.0123	1882.9187	50		146	162	0	(R) (Y)
1941.0006	1940.9792	11		336	351	1	(K) (M)
2083.1040	2083.0249	38		512	528	0	(K) (I)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 18% (123/662AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 12/97 matches (12%).
Acc. #: Species: HUMAN Name: DEAD-box protein 3, Y-chromosomal
Index: MW: 73095 Da pI: 7.0

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1157.5739 1157.5876 -12 362 371 0 (R) (G)
1250.6188 1250.6394 -16 110 119 1 (R) (S)
1329.7389 1329.6837 42 1Met-ox 361 371 1 (R)RIVEQDTMPPK(G)
1336.6384 1336.6394 -0.68 350 360 0 (R) (R)
1340.7499 1340.7578 -5.9 pyroGlu 263 274 0 (K)QYPISLVLAPTR(E)
1357.7228 1357.7844 -45 263 274 0 (K) (E)
1368.6148 1368.6292 -10 2Met-ox 350 360 0 (R)MLDMGFEPQIR(R)
1448.8257 1448.7749 35 275 286 1 (R) (F)
1883.0123 1882.9187 50 144 160 0 (R) (Y)
1941.0006 1940.9792 11 334 349 1 (K) (M)
2023.0858 2023.1664 -40 490 509 1 (K) (H)
2083.1040 2083.0249 38 510 526 0 (R) (I)

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
1157.5739	1157.5876	-12		362	371	0	(R) (G)
1250.6188	1250.6394	-16		110	119	1	(R) (S)
1329.7389	1329.6837	42	1Met-ox	361	371	1	(R)RIVEQDTMPPK(G)
1336.6384	1336.6394	-0.68		350	360	0	(R) (R)
1340.7499	1340.7578	-5.9	pyroGlu	263	274	0	(K)QYPISLVLAPTR(E)
1357.7228	1357.7844	-45		263	274	0	(K) (E)
1368.6148	1368.6292	-10	2Met-ox	350	360	0	(R)MLDMGFEPQIR(R)
1448.8257	1448.7749	35		275	286	1	(R) (F)
1883.0123	1882.9187	50		144	160	0	(R) (Y)
1941.0006	1940.9792	11		334	349	1	(K) (M)
2023.0858	2023.1664	-40		490	509	1	(K) (H)
2083.1040	2083.0249	38		510	526	0	(R) (I)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 19% (126/660AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 12/97 matches (12%).
Acc. #: Species: MOUSE Name: Putative ATP-dependent RNA helicase PL10
Index: MW: 73141 Da pI: 6.7

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1074.5124 1074.5472 -32 418 426 0 (K) (R)
1157.5739 1157.5876 -12 363 372 0 (R) (G)
1230.7367 1230.6483 72 418 427 1 (K) (S)
1329.7389 1329.6837 42 1Met-ox 362 372 1 (R)RIVEQDTMPPK(G)
1336.6384 1336.6394 -0.68 351 361 0 (R) (R)
1340.7499 1340.7578 -5.9 pyroGlu 264 275 0 (K)QYPISLVLAPTR(E)
1357.7228 1357.7844 -45 264 275 0 (K) (E)
1368.6148 1368.6292 -10 2Met-ox 351 361 0 (R)MLDMGFEPQIR(R)
1448.8257 1448.7749 35 276 287 1 (R) (F)
1883.0123 1882.9187 50 145 161 0 (R) (Y)
1941.0006 1940.9792 11 335 350 1 (K) (M)
2083.1040 2083.0249 38 511 527 0 (K) (I)

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
1074.5124	1074.5472	-32		418	426	0	(K) (R)
1157.5739	1157.5876	-12		363	372	0	(R) (G)
1230.7367	1230.6483	72		418	427	1	(K) (S)
1329.7389	1329.6837	42	1Met-ox	362	372	1	(R)RIVEQDTMPPK(G)
1336.6384	1336.6394	-0.68		351	361	0	(R) (R)
1340.7499	1340.7578	-5.9	pyroGlu	264	275	0	(K)QYPISLVLAPTR(E)
1357.7228	1357.7844	-45		264	275	0	(K) (E)
1368.6148	1368.6292	-10	2Met-ox	351	361	0	(R)MLDMGFEPQIR(R)
1448.8257	1448.7749	35		276	287	1	(R) (F)
1883.0123	1882.9187	50		145	161	0	(R) (Y)
1941.0006	1940.9792	11		335	350	1	(K) (M)
2083.1040	2083.0249	38		511	527	0	(K) (I)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 16% (106/660AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):