MS-Fit Search Results

MS-Fit Search Results

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Parameters

Database searched: SwissProt.01.22.2003
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): 40-73
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 121920
Molecular weight search (10000 - 100000 Da) selects 104019 entries.
Full pI range: 121920 entries.
Species search ( HUMAN MOUSE ) selects 14893 entries.
Combined molecular weight, pI and species searches select 12734 entries.
Pre searches select 12734 entries.

Data Set 1 Results

MS-Fit search selects 510 entries (results displayed for top 5 matches).

Results Summary

	MOWSE Score	#/60(%) Masses Matched	% Cov	% TIC	Mean Err ppm	Data Tol ppm	MS-Digest Index #	Protein MW (Da)/pI	Accession #	Species	Protein Name
1	5.400e+005	9 (15)	23.0	15.0	-1.59	34.8		66018/8.2		HUMAN	Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
2	2332	7 (11)	13.0	11.7	-9.77	30.3		80799/9.4		MOUSE	Metastasis-associated protein MTA1
3	1813	8 (13)	15.0	13.3	-2.71	49.1		80789/9.4		HUMAN	Metastasis-associated protein MTA1
4	808	5 (8)	8.0	8.3	16.3	26.9		87960/6.0		HUMAN	Zinc finger protein 151 (Myc-interacting zinc finger protein) (Miz-1 protein)
5	770	6 (10)	12.0	10.0	-2.64	40.5		72038/5.8		MOUSE	INTERFERON-INDUCED GTP-BINDING PROTEIN MX1 (INFLUENZA RESISTANCE PROTEIN)

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Detailed Results

1. 9/60 matches (15%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
Index: MW: 66018 Da pI: 8.2

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
1179.6065	1179.6010	4.7		377	386	0	(K) (H)
1277.7160	1277.7105	4.3		473	483	0	(K) (T)
1300.5482	1300.5302	14		258	267	0	(K) (N)
1302.6649	1302.7018	-28		393	403	1	(R) (V)
1302.6649	1302.7157	-39		344	355	0	(R) (A)
1383.6906	1383.6909	-0.19		186	197	0	(K) (V)
1475.7785	1475.7494	20		212	223	0	(K) (T)
1475.7785	1475.7858	-4.9		200	211	0	(R) (W)
1657.8046	1657.7934	6.7		13	29	0	(R) (R)
1993.9735	1993.9772	-1.8		224	239	0	(R) (R)
2383.9700	2383.9524	7.4		519	549	0	(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 23% (154/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 7/60 matches (11%).
Acc. #: Species: MOUSE Name: Metastasis-associated protein MTA1
Index: MW: 80799 Da pI: 9.4

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
864.4722 864.4678 5.0 671 678 0 (K) (R)
1179.6065 1179.6261 -17 173 182 0 (R) (E)
1307.6870 1307.6748 9.3 198 208 0 (K) (Q)
1507.7445 1507.7678 -15 126 138 0 (K) (S)
1712.7634 1712.8246 -36 1Met-ox 319 331 0 (K)SLTSIIEYYYMWK(T)
1925.8741 1925.8962 -11 144 158 0 (R) (T)
2705.1835 2705.1929 -3.5 283 305 1 (R) (D)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 13% (93/715AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 8/60 matches (13%).
Acc. #: Species: HUMAN Name: Metastasis-associated protein MTA1
Index: MW: 80789 Da pI: 9.4

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
864.4722 864.4678 5.0 671 678 0 (K) (R)
1179.6065 1179.6261 -17 173 182 0 (R) (E)
1182.5792 1182.5690 8.6 1Met-ox 446 456 0 (R)SNMSPHGLPAR(S)
1507.7445 1507.7678 -15 126 138 0 (K) (S)
1712.7634 1712.8246 -36 1Met-ox 319 331 0 (K)SLTSIIEYYYMWK(T)
1925.8741 1925.8962 -11 144 158 0 (R) (T)
2299.1617 2299.0527 47 648 666 1 (R) (K)
2705.1835 2705.1929 -3.5 283 305 1 (R) (D)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 15% (112/715AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 5/60 matches (8%).
Acc. #: Species: HUMAN Name: Zinc finger protein 151 (Myc-interacting zinc finger protein) (Miz-1 protein)
Index: MW: 87960 Da pI: 6.0

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1517.7547 1517.7171 25 551 562 0 (R) (C)
1837.9814 1837.9635 9.8 604 618 0 (K) (C)
1911.9907 1911.9322 31 495 509 0 (R) (Q)
2225.1245 2225.1323 -3.5 604 621 1 (K) (G)
2299.1617 2299.1166 20 113 136 1 (K) (A)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 8% (69/803AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 6/60 matches (10%).
Acc. #: Species: MOUSE Name: INTERFERON-INDUCED GTP-BINDING PROTEIN MX1 (INFLUENZA RESISTANCE PROTEIN)
Index: MW: 72038 Da pI: 5.8

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1111.5670 1111.5934 -24 1Met-ox 336 344 1 (R)TRMSFLVNK(I)
1307.6870 1307.6703 13 584 594 0 (K) (C)
1323.6776 1323.6652 9.3 1Met-ox 584 594 0 (K)MMLQLLQDTSK(C)
1475.7785 1475.7858 -4.9 112 126 0 (K) (L)
1475.7785 1475.7892 -7.2 1Met-ox 428 440 1 (R)VKALEESAVNMLR(R)
1962.9171 1962.9773 -31 248 264 0 (R) (E)
2225.1245 2225.0667 26 373 389 1 (K) (K)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 12% (82/631AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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