MS-Fit Search Results

Parameters

Database searched: SwissProt.01.22.2003
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): 40-77
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 121920
Molecular weight search (1000 - 100000 Da) selects 115277 entries.
Full pI range: 121920 entries.
Species search ( HUMAN MOUSE ) selects 14893 entries.
Combined molecular weight, pI and species searches select 13163 entries.
Pre searches select 13163 entries.

Data Set 1 Results

MS-Fit search selects 509 entries (results displayed for top 5 matches).

Results Summary

	MOWSE Score	#/60(%) Masses Matched	% Cov	% TIC	Mean Err ppm	Data Tol ppm	Protein MW (Da)/pI	Species	Protein Name
1	3.288e+006	12 (20)	22.0	20.0	-21.3	35.1	61988/5.1	HUMAN	Keratin, type I cytoskeletal 9 (Cytokeratin 9) (K9) (CK 9)
2	8.201e+005	13 (21)	50.0	21.7	-22.4	21.8	35854/6.5	HUMAN	Aldose reductase (AR) (Aldehyde reductase)
3	6.556e+005	10 (16)	24.0	16.7	-15.0	56.0	66018/8.2	HUMAN	Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
4	1984	6 (10)	38.0	10.0	-13.5	34.9	18622/5.8	MOUSE	Heat-shock protein, beta-7 (Cardiovascular heat shock protein) (cvHsp) (Heat shock protein 25 kDa 2) (Protein P19/6.8)
5	820	5 (8)	10.0	8.3	-22.6	35.7	72038/5.8	MOUSE	INTERFERON-INDUCED GTP-BINDING PROTEIN MX1 (INFLUENZA RESISTANCE PROTEIN)

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Detailed Results

1. 12/60 matches (20%).
Acc. #: Species: HUMAN Name: Keratin, type I cytoskeletal 9 (Cytokeratin 9) (K9) (CK 9)
Index: MW: 61988 Da pI: 5.1

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
1060.5485	1060.5639	-15		224	232	0	(K) (M)
1065.4673	1065.4999	-31		154	162	0	(K) (L)
1095.5024	1095.5475	-41		184	191	1	(K) (G)
1140.5492	1140.5649	-14	pyroGlu	250	260	0	(R)QGVDADINGLR(Q)
1157.5679	1157.5915	-20		250	260	0	(R) (Q)
1307.6462	1307.6782	-24		240	249	1	(R) (Q)
1323.6352	1323.6731	-29	1Met-ox	240	249	1	(R)IKFEMEQNLR(Q)
1791.7328	1791.7283	2.5		490	512	0	(R) (G)
1837.9312	1837.9660	-19		374	389	0	(R) (K)
2221.1025	2221.0672	16	1Met-ox	242	260	1	(K)FEMEQNLRQGVDADINGLR(Q)
2510.0204	2510.1323	-45		449	471	0	(K) (I)
2705.0622	2705.1617	-37		63	94	0	(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 22% (141/622AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 13/60 matches (21%).
Acc. #: Species: HUMAN Name: Aldose reductase (AR) (Aldehyde reductase)
Index: MW: 35854 Da pI: 6.5

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
892.4716 892.4892 -20 34 41 0 (K) (H)
901.5340 901.5471 -15 244 251 0 (K) (F)
1083.4215 1083.4424 -19 309 316 0 (K) (-)
1112.5615 1112.5952 -30 23 33 0 (K) (V)
1120.6136 1120.6366 -21 70 78 1 (K) (L)
1150.5028 1150.5355 -28 79 86 0 (K) (G)
1234.6468 1234.6465 0.22 91 101 1 (K) (L)
1303.6379 1303.6503 -9.5 298 308 0 (R) (D)
1576.8321 1576.8634 -20 1Met-ox 244 256 1 (K)TTAQVLIRFPMQR(N)
2232.0514 2232.1123 -27 178 195 0 (K) (L)
2282.0043 2282.0651 -27 1Met-ox 276 294 0 (K)VFDFELSSQDMTTLLSYNR(N)
2452.2345 2452.3386 -42 1Met-ox 156 177 0 (K)AIGISNFNHLQVEMILNKPGLK(Y)
2467.0858 2467.1676 -33 42 62 0 (R) (L)

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
892.4716	892.4892	-20		34	41	0	(K) (H)
901.5340	901.5471	-15		244	251	0	(K) (F)
1083.4215	1083.4424	-19		309	316	0	(K) (-)
1112.5615	1112.5952	-30		23	33	0	(K) (V)
1120.6136	1120.6366	-21		70	78	1	(K) (L)
1150.5028	1150.5355	-28		79	86	0	(K) (G)
1234.6468	1234.6465	0.22		91	101	1	(K) (L)
1303.6379	1303.6503	-9.5		298	308	0	(R) (D)
1576.8321	1576.8634	-20	1Met-ox	244	256	1	(K)TTAQVLIRFPMQR(N)
2232.0514	2232.1123	-27		178	195	0	(K) (L)
2282.0043	2282.0651	-27	1Met-ox	276	294	0	(K)VFDFELSSQDMTTLLSYNR(N)
2452.2345	2452.3386	-42	1Met-ox	156	177	0	(K)AIGISNFNHLQVEMILNKPGLK(Y)
2467.0858	2467.1676	-33		42	62	0	(R) (L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 50% (159/316AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 10/60 matches (16%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
Index: MW: 66018 Da pI: 8.2

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
973.4905 973.5318 -42 396 403 0 (K) (V)
1157.5679 1157.5149 46 1Met-ox 464 472 0 (R)DYQELMNTK(L)
1179.5733 1179.6010 -23 377 386 0 (K) (H)
1277.6788 1277.6337 35 247 257 1 (K) (N)
1277.6788 1277.7105 -25 473 483 0 (K) (T)
1300.5243 1300.5302 -4.6 258 267 0 (K) (N)
1383.6534 1383.6909 -27 186 197 0 (K) (V)
1475.7239 1475.7494 -17 212 223 0 (K) (T)
1475.7239 1475.7858 -42 200 211 0 (R) (W)
1716.8203 1716.8517 -18 418 432 0 (K) (G)
1993.9176 1993.9772 -30 224 239 0 (R) (R)
2383.8774 2383.9524 -31 519 549 0 (R) (G)

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
973.4905	973.5318	-42		396	403	0	(K) (V)
1157.5679	1157.5149	46	1Met-ox	464	472	0	(R)DYQELMNTK(L)
1179.5733	1179.6010	-23		377	386	0	(K) (H)
1277.6788	1277.6337	35		247	257	1	(K) (N)
1277.6788	1277.7105	-25		473	483	0	(K) (T)
1300.5243	1300.5302	-4.6		258	267	0	(K) (N)
1383.6534	1383.6909	-27		186	197	0	(K) (V)
1475.7239	1475.7494	-17		212	223	0	(K) (T)
1475.7239	1475.7858	-42		200	211	0	(R) (W)
1716.8203	1716.8517	-18		418	432	0	(K) (G)
1993.9176	1993.9772	-30		224	239	0	(R) (R)
2383.8774	2383.9524	-31		519	549	0	(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 24% (157/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 6/60 matches (10%).
Acc. #: Species: MOUSE Name: Heat-shock protein, beta-7 (Cardiovascular heat shock protein) (cvHsp) (Heat shock protein 25 kDa 2) (Protein P19/6.8)
Index: MW: 18622 Da pI: 5.8

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1090.5108 1090.5394 -26 AcetN 1 10 1 (-)SHRTSSAFR(A)
1117.5635 1117.5966 -30 142 151 1 (R) (R)
1179.5733 1179.5693 3.4 1 10 1 (-) (A)
1475.7239 1475.7317 -5.2 111 124 0 (K) (C)
1493.6788 1493.7249 -31 153 164 0 (R) (T)
2246.1265 2246.1093 7.6 89 107 0 (R) (A)

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
1090.5108	1090.5394	-26	AcetN	1	10	1	(-)SHRTSSAFR(A)
1117.5635	1117.5966	-30		142	151	1	(R) (R)
1179.5733	1179.5693	3.4		1	10	1	(-) (A)
1475.7239	1475.7317	-5.2		111	124	0	(K) (C)
1493.6788	1493.7249	-31		153	164	0	(R) (T)
2246.1265	2246.1093	7.6		89	107	0	(R) (A)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 38% (65/169AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 5/60 matches (8%).
Acc. #: Species: MOUSE Name: INTERFERON-INDUCED GTP-BINDING PROTEIN MX1 (INFLUENZA RESISTANCE PROTEIN)
Index: MW: 72038 Da pI: 5.8

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1150.5028 1150.5017 0.99 326 335 0 (K) (T)
1307.6462 1307.6703 -18 584 594 0 (K) (C)
1323.6352 1323.6652 -23 1Met-ox 584 594 0 (K)MMLQLLQDTSK(C)
1475.7239 1475.7858 -42 112 126 0 (K) (L)
1475.7239 1475.7892 -44 1Met-ox 428 440 1 (R)VKALEESAVNMLR(R)
2225.0463 2225.0667 -9.2 373 389 1 (K) (K)

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
1150.5028	1150.5017	0.99		326	335	0	(K) (T)
1307.6462	1307.6703	-18		584	594	0	(K) (C)
1323.6352	1323.6652	-23	1Met-ox	584	594	0	(K)MMLQLLQDTSK(C)
1475.7239	1475.7858	-42		112	126	0	(K) (L)
1475.7239	1475.7892	-44	1Met-ox	428	440	1	(R)VKALEESAVNMLR(R)
2225.0463	2225.0667	-9.2		373	389	1	(K) (K)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 10% (66/631AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):