MS-Fit Search Results

MS-Fit Search Results

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Parameters

Database searched: SwissProt.01.22.2003
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): 40-80
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 121920
Molecular weight search (10000 - 100000 Da) selects 104019 entries.
Full pI range: 121920 entries.
Species search ( HUMAN MOUSE ) selects 14893 entries.
Combined molecular weight, pI and species searches select 12734 entries.
Pre searches select 12734 entries.

Data Set 1 Results

MS-Fit search selects 47 entries (results displayed for top 5 matches).

Results Summary

	MOWSE Score	#/25(%) Masses Matched	% Cov	% TIC	Mean Err ppm	Data Tol ppm	MS-Digest Index #	Protein MW (Da)/pI	Accession #	Species	Protein Name
1	8.327e+005	9 (36)	33.0	36.0	32.9	48.8		38093/6.3		HUMAN	Voltage-dependent anion-selective channel protein 2 (VDAC-2) (hVDAC2) (Outer mitochondrial membrane protein porin 2)
2	3.417e+004	7 (28)	29.0	28.0	33.3	43.2		31733/7.4		MOUSE	Voltage-dependent anion-selective channel protein 2 (VDAC-2) (mVDAC2) (mVDAC6) (Outer mitochondrial membrane protein porin 2)
3	156	4 (16)	25.0	16.0	35.4	48.0		31515/5.8		HUMAN	Interferon-induced 35 kDa protein (IFP 35)
4	125	4 (16)	17.0	16.0	-35.0	59.4		35738/8.0		HUMAN	ADP-ribosyl cyclase 2 precursor (Cyclic ADP-ribose hydrolase 2) (cADPr hydrolase 2) (Bone marrow stromal antigen 1) (BST-1) (CD157 antigen)
5	121	4 (16)	9.0	16.0	34.2	37.1		92379/8.2		MOUSE	Semaphorin 4G precursor

94510	.	.	x	.	.	.	.	.	x	.	.	.	x	x	x	.	x	.	x	x	.	.	.	.	x
88822	.	.	.	.	.	.	.	.	x	.	.	.	x	x	x	.	x	.	x	x	.	.	.	.	.
16590	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	x	x	.	.	x	.
26896	.	.	.	x	.	.	x	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	x
72507	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	x	.	.	x	x

Detailed Results

1. 9/25 matches (36%).
Acc. #: Species: HUMAN Name: Voltage-dependent anion-selective channel protein 2 (VDAC-2) (hVDAC2) (Outer mitochondrial membrane protein porin 2)
Index: MW: 38093 Da pI: 6.3

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
940.4643	940.4641	0.26		193	200	0	(R) (T)
1016.5954	1016.5992	-3.7		283	292	0	(K) (S)
1293.7042	1293.6691	27		251	262	0	(K) (V)
1428.7459	1428.7011	31		123	135	0	(K) (K)
1447.6888	1447.6568	22		90	100	0	(K) (W)
1556.8606	1556.7960	41		123	136	1	(K) (S)
1921.9269	1921.8085	62		61	79	0	(K) (V)
2103.2678	2103.1562	53		263	282	0	(K) (L)
2528.3281	2528.1694	63		201	223	0	(R) (V)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 33% (117/347AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 7/25 matches (28%).
Acc. #: Species: MOUSE Name: Voltage-dependent anion-selective channel protein 2 (VDAC-2) (mVDAC2) (mVDAC6) (Outer mitochondrial membrane protein porin 2)
Index: MW: 31733 Da pI: 7.4

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1016.5954 1016.5992 -3.7 269 278 0 (K) (S)
1293.7042 1293.6691 27 237 248 0 (K) (V)
1428.7459 1428.7011 31 109 121 0 (K) (K)
1447.6888 1447.6568 22 76 86 0 (K) (W)
1556.8606 1556.7960 41 109 122 1 (K) (S)
1921.9269 1921.8085 62 47 65 0 (K) (V)
2103.2678 2103.1562 53 249 268 0 (K) (L)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 29% (86/295AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 4/25 matches (16%).
Acc. #: Species: HUMAN Name: Interferon-induced 35 kDa protein (IFP 35)
Index: MW: 31515 Da pI: 5.8

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1016.5954 1016.5781 17 39 47 1 (K) (I)
2103.2678 2103.1198 70 AcetN 1 20 1 (-)SAPLDAALHALQEEQARLK(M)
2125.2245 2125.1592 31 192 210 0 (R) (V)
2517.3061 2517.2473 23 261 286 0 (R) (-)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 25% (74/286AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 4/25 matches (16%).
Acc. #: Species: HUMAN Name: ADP-ribosyl cyclase 2 precursor (Cyclic ADP-ribose hydrolase 2) (cADPr hydrolase 2) (Bone marrow stromal antigen 1) (BST-1) (CD157 antigen)
Index: MW: 35738 Da pI: 8.0

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
941.4596 941.4805 -22 36 44 0 (R) (D)
952.4590 952.5216 -66 174 181 1 (K) (D)
1921.9269 1921.9264 0.23 268 283 0 (K) (S)
2528.3281 2528.4605 -52 293 315 1 (K) (T)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 17% (56/318AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 4/25 matches (16%).
Acc. #: Species: MOUSE Name: Semaphorin 4G precursor
Index: MW: 92379 Da pI: 8.2

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1556.8606 1556.8259 22 290 301 0 (R) (G)
2125.2245 2125.0930 62 382 400 1 (R) (L)
2517.3061 2517.2408 26 800 822 1 (R) (R)
2528.3281 2528.2608 27 163 185 1 (K) (Y)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 9% (77/837AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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