MS-Fit Search Results



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Parameters

Database searched: SwissProt.01.22.2003
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): 40-81
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 121920
Molecular weight search (1000 - 100000 Da) selects 115277 entries.
Full pI range: 121920 entries.
Species search ( HUMAN MOUSE ) selects 14893 entries.
Combined molecular weight, pI and species searches select 13163 entries.
Pre searches select 13163 entries.

Data Set 1 Results

MS-Fit search selects 1021 entries (results displayed for top 5 matches).

Results Summary

MOWSE
Score
#/60(%)
Masses
Matched
%
Cov
%
TIC
Mean
Err
ppm
Data
Tol
ppm
MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
15.015e+00716 (26)61.026.747.518.9 35077/7.6HUMANGuanine nucleotide-binding protein beta subunit-like protein 12.3 (P205) (Receptor of activated protein kinase C 1) (RACK1) (Receptor for activated C kinase)
27.103e+00410 (16)25.016.729.745.5 55622/9.4MOUSESpastin
32.766e+0046 (10)16.010.047.422.9 66018/8.2HUMANKeratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
41.501e+00410 (16)13.016.77.9471.0 80789/9.4HUMANMetastasis-associated protein MTA1
51.500e+00410 (16)13.016.718.665.2 80799/9.4MOUSEMetastasis-associated protein MTA1
85079x..x.x..x.x.........x..x......x..x.x.x..x....x..x......x..x.
40207....x.x...x..x.......x.......xx..........x.............x...x
48753...................x.....x........x...x........x........x...
55299.x....x..x.........x...xx...x.x.....x............x..........
36457.x....x..x.........x..xx.....xx.....x............x..........



Detailed Results

1. 16/60 matches (26%).
Acc. #: Species: HUMAN Name: Guanine nucleotide-binding protein beta subunit-like protein 12.3 (P205) (Receptor of activated protein kinase C 1) (RACK1) (Receptor for activated C kinase)
Index: MW: 35077 Da pI: 7.6
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
860.4855860.4518392582640(K) (I)
936.4771936.446133AcetN 1Met-ox 180(-)TEQMTLR(G)
1018.55361018.5144381761830(K) (L)
1050.59221050.5658251Met-ox 37441(R)DKTIIMWK(L)
1059.64641059.5951483093170(R) (-)
1192.62161192.55985248570(R) (A)
1264.71221264.65374689990(R) (R)
1309.70761309.6388531071180(K) (Q)
1380.76231380.6986462462570(R) (I)
1492.76211492.6742591Met-ox 2132250(K)DGQAMLWDLNEGK(H)
1562.87411562.79275245571(K) (A)
1789.08271788.9959492652801(K) (A)
1922.01041921.9118511561720(R) (L)
1995.99971995.8871561401550(K) (F)
2290.26852290.1290612262450(K) (Y)
2643.43262643.2989511Met-ox 13360(K)GHNGWVTQIATTPQFPDMILSASR(D)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 61% (194/317AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 10/60 matches (16%).
Acc. #: Species: MOUSE Name: Spastin
Index: MW: 55622 Da pI: 9.4
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1014.63161014.59483678851(K) (L)
1039.53541039.53124.04704780(R) (K)
1059.64641059.62032586940(K) (S)
1072.62341072.590431191(-) (Q)
1206.64141206.6006342292390(K) (Q)
1307.74351307.6708561251360(K) (S)
1309.70761309.64314969791(K) (L)
1791.90211791.9394-214834971(R) (D)
2290.26852290.1641463203380(R) (R)
2644.46442644.3656372773021(K) (Y)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 25% (127/504AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 6/60 matches (10%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
Index: MW: 66018 Da pI: 8.2
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1179.65071179.6010423773860(K) (H)
1277.74791277.7105294734830(K) (T)
1475.84251475.7494632122230(K) (T)
1475.84251475.7858382002110(R) (W)
1716.93821716.8517504184320(K) (G)
1994.08531993.9772542242390(R) (R)
2384.08242383.9524555195490(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 16% (107/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 10/60 matches (16%).
Acc. #: Species: HUMAN Name: Metastasis-associated protein MTA1
Index: MW: 80789 Da pI: 9.4
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
864.5134864.4678536716780(K) (R)
1039.53541039.5246104574661(R) (T)
1057.59051057.5907-0.211051121(R) (Q)
1179.65071179.6261211731820(R) (E)
1264.71221264.70148.61131230(R) (G)
1275.71071275.7160-4.25555670(K) (V)
1300.67201300.7265-424985090(R) (A)
1309.70761309.632357pyroGlu 2352470(R)QPSLHMSAAAASR(D)
1507.81001507.7678281261380(K) (S)
2010.00862010.1136-521071231(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 13% (98/715AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 10/60 matches (16%).
Acc. #: Species: MOUSE Name: Metastasis-associated protein MTA1
Index: MW: 80799 Da pI: 9.4
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
864.5134864.4678536716780(K) (R)
1039.53541039.5246104574661(R) (T)
1057.59051057.5907-0.211051121(R) (Q)
1179.65071179.6261211731820(R) (E)
1263.68981263.67968.15555670(K) (S)
1264.71221264.70148.61131230(R) (G)
1307.74351307.6748531982080(K) (Q)
1309.70761309.632357pyroGlu 2352470(R)QPSLHMSAAAASR(D)
1507.81001507.7678281261380(K) (S)
2010.00862010.1136-521071231(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 13% (97/715AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):


MS-Fit in ProteinProspector 4.0.4
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