MS-Fit Search Results

MS-Fit Search Results

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Parameters

Database searched: SwissProt.01.22.2003
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): 40-82
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 121920
Molecular weight search (1000 - 100000 Da) selects 115277 entries.
Full pI range: 121920 entries.
Species search ( HUMAN MOUSE ) selects 14893 entries.
Combined molecular weight, pI and species searches select 13163 entries.
Pre searches select 13163 entries.

Data Set 1 Results

MS-Fit search selects 823 entries (results displayed for top 5 matches).

Results Summary

	MOWSE Score	#/60(%) Masses Matched	% Cov	% TIC	Mean Err ppm	Data Tol ppm	MS-Digest Index #	Protein MW (Da)/pI	Accession #	Species	Protein Name
1	1.724e+007	13 (21)	40.0	21.7	42.6	34.9		35993/8.7		MOUSE	Heterogeneous nuclear ribonucleoproteins A2/B1 (hnRNP A2 / hnRNP B1)
2	1.658e+007	13 (21)	38.0	21.7	42.6	34.9		37430/9.0		HUMAN	Heterogeneous nuclear ribonucleoproteins A2/B1 (hnRNP A2 / hnRNP B1)
3	3.686e+005	10 (16)	20.0	16.7	32.8	37.6		59519/5.1		HUMAN	Keratin, type I cytoskeletal 10 (Cytokeratin 10) (K10) (CK 10)
4	1.026e+005	10 (16)	23.0	16.7	14.5	71.7		73244/6.7		HUMAN	DEAD-box protein 3 (Helicase-like protein 2) (HLP2) (DEAD-box, X isoform)
5	6.550e+004	8 (13)	16.0	13.3	30.4	44.8		66018/8.2		HUMAN	Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)

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119594	x	.	x	.	x	.	.	.	.	x	.	.	.	.	.	x	.	x	.	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	x	.	x	x	x	.	.	.	.	.	.	.	.	.	x	.	x	.	.	.	.	.	.	.
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48753	.	.	.	.	x	.	.	.	x	.	.	x	x	.	.	.	x	.	.	.	x	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	x	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.	.

Detailed Results

1. 13/60 matches (21%).
Acc. #: Species: MOUSE Name: Heterogeneous nuclear ribonucleoproteins A2/B1 (hnRNP A2 / hnRNP B1)
Index: MW: 35993 Da pI: 8.7

m/z Submitted	MH⁺ Matched	Delta ppm	Modifications	Start	End	Missed Cleavages	Database Sequence
1013.4667	1013.4441	22		192	201	0	(R) (G)
1050.4671	1050.4420	24		118	125	0	(R) (I)
1087.5072	1087.4849	21		27	34	0	(R) (L)
1188.6705	1188.6476	19		126	135	0	(K) (Q)
1377.6833	1377.6300	39		202	216	0	(R) (G)
1410.7443	1410.6878	40		162	173	0	(K) (K)
1695.8445	1695.7655	47		142	156	0	(R) (I)
1799.0081	1798.9227	47		11	26	0	(K) (N)
1852.0000	1851.8666	72		141	156	1	(K) (I)
1880.0574	1879.9666	48		102	117	1	(K) (D)
1927.1153	1927.0177	51		10	26	1	(R) (N)
2206.0530	2205.9008	69	1Met-ox	314	338	0	(R)NMGGPYGGGNYGPGGSGGSGGYGGR(S)
2220.1921	2220.0712	54		118	135	1	(R) (Q)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 40% (137/341AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 13/60 matches (21%).
Acc. #: Species: HUMAN Name: Heterogeneous nuclear ribonucleoproteins A2/B1 (hnRNP A2 / hnRNP B1)
Index: MW: 37430 Da pI: 9.0

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1013.4667 1013.4441 22 204 213 0 (R) (G)
1050.4671 1050.4420 24 130 137 0 (R) (I)
1087.5072 1087.4849 21 39 46 0 (R) (L)
1188.6705 1188.6476 19 138 147 0 (K) (Q)
1377.6833 1377.6300 39 214 228 0 (R) (G)
1410.7443 1410.6878 40 174 185 0 (K) (K)
1695.8445 1695.7655 47 154 168 0 (R) (I)
1799.0081 1798.9227 47 23 38 0 (K) (N)
1852.0000 1851.8666 72 153 168 1 (K) (I)
1880.0574 1879.9666 48 114 129 1 (K) (D)
1927.1153 1927.0177 51 22 38 1 (R) (N)
2206.0530 2205.9008 69 1Met-ox 326 350 0 (R)NMGGPYGGGNYGPGGSGGSGGYGGR(S)
2220.1921 2220.0712 54 130 147 1 (R) (Q)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 38% (137/353AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 10/60 matches (16%).
Acc. #: Species: HUMAN Name: Keratin, type I cytoskeletal 10 (Cytokeratin 10) (K10) (CK 10)
Index: MW: 59519 Da pI: 5.1

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1090.5482 1090.5315 15 148 156 0 (K) (L)
1109.5197 1109.4904 26 335 343 0 (K) (S)
1234.7138 1234.6796 28 236 245 1 (R) (Q)
1300.5861 1300.5956 -7.3 286 295 1 (K) (N)
1365.6962 1365.6399 41 323 333 0 (R) (K)
1434.8182 1434.7705 33 440 450 1 (K) (S)
1493.7947 1493.7348 40 323 334 1 (R) (D)
1707.8668 1707.7727 55 41 59 0 (K) (G)
2097.0655 2096.9810 40 423 439 0 (R) (I)
2872.5535 2872.3933 56 296 322 0 (R) (S)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 20% (124/593AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 10/60 matches (16%).
Acc. #: Species: HUMAN Name: DEAD-box protein 3 (Helicase-like protein 2) (HLP2) (DEAD-box, X isoform)
Index: MW: 73244 Da pI: 6.7

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1090.5482 1090.5421 5.6 419 427 0 (K) (R)
1300.5861 1300.6398 -41 479 488 1 (R) (S)
1320.6349 1320.6800 -34 277 287 0 (R) (K)
1707.8668 1707.8964 -17 312 326 1 (R) (L)
1795.8930 1795.7847 60 601 617 1 (R) (A)
1839.0177 1838.9513 36 532 548 1 (R) (N)
1941.0454 1940.9792 34 336 351 1 (K) (M)
2083.1299 2083.0249 50 512 528 0 (K) (I)
2145.1564 2145.1351 9.9 293 311 1 (R) (D)
2662.5390 2662.4278 42 231 255 1 (K) (E)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 23% (156/662AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 8/60 matches (13%).
Acc. #: Species: HUMAN Name: Keratin, type II cytoskeletal 1 (Cytokeratin 1) (K1) (CK 1) (67 kDa cytokeratin) (Hair alpha protein)
Index: MW: 66018 Da pI: 8.2

m/z
Submitted MH⁺
Matched Delta
ppm Modifications Start End Missed
Cleavages Database
Sequence
1087.5072 1087.5285 -20 4 12 1 (R) (S)
1179.6385 1179.6010 32 377 386 0 (K) (H)
1277.7375 1277.7105 21 473 483 0 (K) (T)
1300.5861 1300.5302 43 258 267 0 (K) (N)
1383.7385 1383.6909 34 186 197 0 (K) (V)
1475.8114 1475.7494 42 212 223 0 (K) (T)
1475.8114 1475.7858 17 200 211 0 (R) (W)
1716.9337 1716.8517 48 418 432 0 (K) (G)
1994.0884 1993.9772 56 224 239 0 (R) (R)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 16% (107/644AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

MS-Fit in ProteinProspector 4.0.4
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