MS-Fit Search Results



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Parameters

Database searched: SwissProt.01.22.2003
Digest Used: Trypsin
Max. # Missed Cleavages: 1
Peptide N terminus: Hydrogen
Peptide C terminus: Free Acid
Cysteine Modification: acrylamide
Instrument Name: MALDI-TOF
Sample ID (comment): 40-83
Minimum Matches: 4
Sort Type: Score Sort
Considered modifications: | Peptide N-terminal Gln to pyroGlu | Oxidation of M | Protein N-terminus Acetylated |
Min Parent Ion Matches: 1
MOWSE On: 1
MOWSE P Factor: 0.4

Pre Search Results

Number of entries in the database: 121920
Molecular weight search (1000 - 100000 Da) selects 115277 entries.
Full pI range: 121920 entries.
Species search ( HUMAN MOUSE ) selects 14893 entries.
Combined molecular weight, pI and species searches select 13163 entries.
Pre searches select 13163 entries.

Data Set 1 Results

MS-Fit search selects 579 entries (results displayed for top 5 matches).

Results Summary

MOWSE
Score
#/67(%)
Masses
Matched
%
Cov
%
TIC
Mean
Err
ppm
Data
Tol
ppm
MS-Digest Index #Protein MW (Da)/pIAccession #SpeciesProtein Name
11.716e+00713 (19)40.019.4-40.430.6 35993/8.7MOUSEHeterogeneous nuclear ribonucleoproteins A2/B1 (hnRNP A2 / hnRNP B1)
21.650e+00713 (19)38.019.4-40.430.6 37430/9.0HUMANHeterogeneous nuclear ribonucleoproteins A2/B1 (hnRNP A2 / hnRNP B1)
32.693e+0046 (8)16.09.0-21.753.1 71289/6.0MOUSEGTP-RHO BINDING PROTEIN 1 (RHOPHILIN)
468406 (8)13.09.0-12.770.8 66268/5.3MOUSEDNA POLYMERASE ALPHA 70 KDA SUBUNIT (DNA POLYMERASE SUBUNIT B)
564909 (13)35.013.4-34.518.6 36054/8.6HUMANGlyceraldehyde 3-phosphate dehydrogenase, liver
81777xxx.x..x..x..............x............x...x.xx............x.x......
119594xxx.x..x..x..............x............x...x.xx............x.x......
110305...................x...x................x.x......x.........x.......
85413........................................xx........x..........xx..x.
117116.....x..........x.x............x...........x......x..........xx..x.



Detailed Results

1. 13/67 matches (19%).
Acc. #: Species: MOUSE Name: Heterogeneous nuclear ribonucleoproteins A2/B1 (hnRNP A2 / hnRNP B1)
Index: MW: 35993 Da pI: 8.7
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1013.38041013.4441-631922010(R) (G)
1050.38291050.4420-561181250(R) (I)
1087.42251087.4849-5727340(R) (L)
1188.58141188.6476-561261350(K) (Q)
1377.56821377.6300-452022160(R) (G)
1410.62331410.6878-461621730(K) (K)
1695.70211695.7655-371421560(R) (I)
1798.85161798.9227-4011260(K) (N)
1851.83521851.8666-171411561(K) (I)
1879.89911879.9666-361021171(K) (D)
1926.95391927.0177-3310261(R) (N)
2205.85952205.9008-191Met-ox 3143380(R)NMGGPYGGGNYGPGGSGGSGGYGGR(S)
2220.02422220.0712-211181351(R) (Q)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 40% (137/341AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

2. 13/67 matches (19%).
Acc. #: Species: HUMAN Name: Heterogeneous nuclear ribonucleoproteins A2/B1 (hnRNP A2 / hnRNP B1)
Index: MW: 37430 Da pI: 9.0
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1013.38041013.4441-632042130(R) (G)
1050.38291050.4420-561301370(R) (I)
1087.42251087.4849-5739460(R) (L)
1188.58141188.6476-561381470(K) (Q)
1377.56821377.6300-452142280(R) (G)
1410.62331410.6878-461741850(K) (K)
1695.70211695.7655-371541680(R) (I)
1798.85161798.9227-4023380(K) (N)
1851.83521851.8666-171531681(K) (I)
1879.89911879.9666-361141291(K) (D)
1926.95391927.0177-3322381(R) (N)
2205.85952205.9008-191Met-ox 3263500(R)NMGGPYGGGNYGPGGSGGSGGYGGR(S)
2220.02422220.0712-211301471(R) (Q)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 38% (137/353AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

3. 6/67 matches (8%).
Acc. #: Species: MOUSE Name: GTP-RHO BINDING PROTEIN 1 (RHOPHILIN)
Index: MW: 71289 Da pI: 6.0
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1614.82951614.82800.913413540(R) (A)
1628.83251628.82842.54784920(K) (N)
1838.83761838.9149-4259741(R) (E)
1851.83521851.82893.44624770(R) (T)
2036.01532036.1028-435165370(R) (E)
2211.01772211.1331-525645840(R) (G)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 16% (104/643AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

4. 6/67 matches (8%).
Acc. #: Species: MOUSE Name: DNA POLYMERASE ALPHA 70 KDA SUBUNIT (DNA POLYMERASE SUBUNIT B)
Index: MW: 66268 Da pI: 5.3
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1838.83761838.9037-361681850(R) (G)
1848.84841848.9206-393703850(R) (H)
2041.03652040.925854pyroGlu 36540(R)QTEDGMVSELIAFCTSAGK(T)
2245.05932245.0963-162933120(K) (R)
2401.11472401.1974-342933131(K) (L)
2611.30012611.3116-4.41681941(R) (V)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 13% (83/600AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):

5. 9/67 matches (13%).
Acc. #: Species: HUMAN Name: Glyceraldehyde 3-phosphate dehydrogenase, liver
Index: MW: 36054 Da pI: 8.6
m/z
Submitted
MH+
Matched
Delta
ppm
ModificationsStartEndMissed
Cleavages
Database
Sequence
1233.55391233.5972-352Met-ox 3243340(R)VVDLMAHMASK(E)
1544.74501544.8107-432352480(R) (L)
1613.83741613.9015-4067800(K) (D)
1763.73931763.8029-363103230(K) (V)
1861.86481861.9516-471461620(K) (V)
2041.03652041.1082-3567841(K) (I)
2245.05932245.0997-182Met-ox 1191390(R)VIISAPSADAPMFVMGVNHEK(Y)
2401.11472401.2008-362Met-ox 1181391(K)RVIISAPSADAPMFVMGVNHEK(Y)
2611.30012611.3554-211Met-ox 1631860(K)VIHDNFGIVEGLMTTVHAITATQK(T)

Click link below to search for cysteine linked fragments.

Click link below to do a non-specific cleavage search.

Click link below to search for another component.

The matched peptides cover 35% (120/335AA's) of the protein.
Coverage Map for This Hit (MS-Digest index #):


MS-Fit in ProteinProspector 4.0.4
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